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anti-NT5E antibody :: Rabbit anti-Rat 5'-Nucleotidase, Ecto (NT5E) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2002958 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $185 +1 FREE 8GB USB
0.1 mg  /  $230 +1 FREE 8GB USB
0.2 mg  /  $350 +1 FREE 8GB USB
1 mg  /  $830 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5'-Nucleotidase, Ecto (NT5E), Polyclonal Antibody

 Also Known As   

Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

 Product Gene Name   

anti-NT5E antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG SEF-WE LTIMHTNDVH SRLEQTSDDS TKCLNASLCV GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG LEVAHFMNLL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAPQISGLY LPYKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEVT ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV VVGGHTNTFL YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG SAQECRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DERNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDISRKPWDR VVQLKVLCTK CRVPIYEPLE MDKVYKVVLP SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI YPAVEGRIKF
 3D Structure    ModBase 3D Structure for P21588
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Rat
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 Specificity    The antibody is a rabbit polyclonal antibody raised against NT5E. It has been selected for its ability to recognize NT5E in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant NT5E (Trp29~phe550) expressed in E.coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2053156
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-NT5E antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-NT5E antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) of anti-NT5E antibody    (Figure. Western Blot; Sample: Recombinant protein.)
anti-NT5E antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-NT5E antibody    (Figure.DAB staining on IHC-P. Samples: Rat Tissue)
anti-NT5E antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.
 NCBI GI #    112826
 NCBI GeneID    58813
 NCBI Accession #    P21588.1 [Other Products]
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 UniProt Primary Accession #    P21588 [Other Products]
 UniProt Related Accession #    P21588 [Other Products]
 Molecular Weight    63,969 Da
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 NCBI Official Full Name    5'-nucleotidase
 NCBI Official Synonym Full Names    5' nucleotidase, ecto
 NCBI Official Symbol    Nt5e [Similar Products]
 NCBI Official Synonym Symbols   
Nt5; CD73
[Similar Products]
 NCBI Protein Information    5'-nucleotidase
 UniProt Protein Name    5'-nucleotidase
 UniProt Synonym Protein Names   
Ecto-5'-nucleotidase; CD_antigen: CD73
 UniProt Gene Name    Nt5e [Similar Products]
 UniProt Synonym Gene Names    Nt5; Nte; 5'-NT [Similar Products]
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 NCBI Summary for NT5E    may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
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 UniProt Comments for NT5E    NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Membrane protein, GPI anchor; Membrane protein, integral; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 8q31

Cellular Component: cell surface; cytosol; integral component of membrane; membrane; plasma membrane

Molecular Function: 5'-nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding

Biological Process: adenosine biosynthetic process; adenosine metabolic process; AMP catabolic process; brain development; dephosphorylation; leukocyte adhesion; negative regulation of inflammatory response; positive regulation of lipid biosynthetic process; purine nucleotide biosynthetic process; response to aluminum ion
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 Research Articles on NT5E    1. Fluvastatin increases adenosine concentrations in the heart ventricles as a result of activation of PKC, mediated by stimulation of alpha1-adrenoceptors, through activation of ecto-5'-nucleotidase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NT5E antibodyOrgans/Tissues associated with anti-NT5E antibody
 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with NT5E and Brain
 Connective Tissue Antibodies  >3 publications with NT5E and Connective Tissue
 Heart Antibodies  >2 publications with NT5E and Heart
 Prostate Antibodies  >1 publications with NT5E and Prostate
 Testis Antibodies  >1 publications with NT5E and Testis
 Liver Antibodies  >1 publications with NT5E and Liver
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