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anti-ACO1 antibody :: Rabbit ACO1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9217191
Western Blot (WB)
Unit / Price
0.05 mL  /  $155 +1 FREE 8GB USB
0.2 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ACO1, Polyclonal Antibody

 Also Known As   

ACO1 Antibody (Center)

 Product Synonym Names    Cytoplasmic aconitate hydratase; Aconitase; 4.2.1.3; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1; ACO1; IREB1
 Product Gene Name   

anti-ACO1 antibody

[Similar Products]
 Product Synonym Gene Name    IREB1 [Similar Products]
 Antibody/Peptide Pairs    ACO1 peptide (MBS9230621) is used for blocking the activity of ACO1 antibody (MBS9217191)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    100880
 3D Structure    ModBase 3D Structure for P21399
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 Clonality    Polyclonal
 Isotype    Ig
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat; Predicted Reactivity: Bovine, Chicken
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 Specificity    This ACO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 354-388 amino acids from the Central region of human ACO1.
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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 Subtitle    Purified Rabbit Polyclonal Antibody (Pab)
 Cellular Location    Cytoplasm.
 Antigen Type    Synthesized Peptide
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-ACO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ACO1 antibody

   Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
 Product Categories/Family for anti-ACO1 antibody    Crown Antibodies; New Antibodies; Primary Antibodies; Cardiovascular; Metabolism; Signal Transduction
 Applications Tested/Suitable for anti-ACO1 antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-ACO1 antibody    WB:~~1:2000
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 Western Blot (WB) of anti-ACO1 antibody    All lanes : Anti-ACO1 Antibody (Center) at 1:2000 dilution
Lane 1: human kidney lysate
Lane 2: human liver lysate
Lane 3: U-2OS whole cell lysate
Lane 4: A431 whole cell lysate
Lane 5: mouse kidney lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 98 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-ACO1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ACO1. It may not necessarily be applicable to this product.
 NCBI GI #    507834063
 NCBI GeneID    48
 NCBI Accession #    NP_001265281.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001278352.1 [Other Products]
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 UniProt Primary Accession #    P21399 [Other Products]
 UniProt Secondary Accession #    Q14652; Q5VZA7; D3DRK7 [Other Products]
 UniProt Related Accession #    P21399 [Other Products]
 Molecular Weight    98399 Da
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 NCBI Official Full Name    cytoplasmic aconitate hydratase
 NCBI Official Synonym Full Names    aconitase 1
 NCBI Official Symbol    ACO1 [Similar Products]
 NCBI Official Synonym Symbols   
IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
[Similar Products]
 NCBI Protein Information    cytoplasmic aconitate hydratase
 UniProt Protein Name    Cytoplasmic aconitate hydratase
 UniProt Synonym Protein Names   
Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
 Protein Family    Aconitate hydratase
 UniProt Gene Name    ACO1 [Similar Products]
 UniProt Synonym Gene Names    IREB1; Aconitase; IRP1; IRE-BP 1 [Similar Products]
 UniProt Entry Name    ACOC_HUMAN
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 NCBI Summary for ACO1    The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
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 UniProt Comments for ACO1    IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: EC 4.2.1.3; Lyase; RNA-binding; Endoplasmic reticulum; Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Translation

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; metal ion binding; protein binding; RNA binding

Biological Process: cellular iron ion homeostasis; citrate metabolic process; intestinal absorption; post-embryonic development; regulation of translation; response to iron(II) ion; tricarboxylic acid cycle
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Product References and Citations for anti-ACO1 antibody

   Hirling H.,et al.Nucleic Acids Res. 20:33-39 (1992). Humphray S.J.,et al.Nature 429:369-374 (2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Rouault T.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962 (1990). Hentze M.W.,et al.Nucleic Acids Res. 19:1739-1740 (1991).
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 Research Articles on ACO1    1. Data show that IRP1 suppressed TfR1/DMT1 (+IRE) expression, limited the cellular iron content and decreased lactate dehydrogenase (LDH) release induced by hypoxia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ACO1 antibodyPathways associated with anti-ACO1 antibody
 Reference Product  PubMed Publications
 ACO2 antibody  >24 publications with ACO1 and ACO2
 IDH1 antibody  >4 publications with ACO1 and IDH1
 IDH2 antibody  >2 publications with ACO1 and IDH2
 FTH1 antibody  >1 publications with ACO1 and FTH1
 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
Diseases associated with anti-ACO1 antibodyOrgans/Tissues associated with anti-ACO1 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with ACO1 and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with ACO1 and Brain Diseases
 Fatty Liver Antibodies  >2 publications with ACO1 and Fatty Liver
 Lung Neoplasms Antibodies  >1 publications with ACO1 and Lung Neoplasms
 Lung Diseases Antibodies  >1 publications with ACO1 and Lung Diseases
 Anemia Antibodies  >1 publications with ACO1 and Anemia
 Nerve Degeneration Antibodies  >1 publications with ACO1 and Nerve Degeneration
 Fibrosis Antibodies  >1 publications with ACO1 and Fibrosis
 Kidney Diseases Antibodies  >1 publications with ACO1 and Kidney Diseases
 Inflammation Antibodies  >1 publications with ACO1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with ACO1 and Liver
 Blood Antibodies  >3 publications with ACO1 and Blood
 Brain Antibodies  >3 publications with ACO1 and Brain
 Skin Antibodies  >2 publications with ACO1 and Skin
 Kidney Antibodies  >2 publications with ACO1 and Kidney
 Muscle Antibodies  >2 publications with ACO1 and Muscle
 Vascular Antibodies  >2 publications with ACO1 and Vascular
 Lung Antibodies  >2 publications with ACO1 and Lung
 Embryonic Tissue Antibodies  >1 publications with ACO1 and Embryonic Tissue
 Eye Antibodies  >1 publications with ACO1 and Eye
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