Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow AFP rightarrow Polyclonal AFP  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • 26S protease regulatory subunit 6B (PSMC4) Recombinant Protein
 • Adenovirus Antibody
 • Agmatinase (speB) Recombinant Protein
 • Ubiquitin (UBB) Antibody
 • CXCL11 Antibody
 • Carnosine ELISA Kit
 • Asialoganglioside GM1 Ganglioside
 • ubiquinol-cytochrome c reductase core protein II (UQCRC2) Antibody
 • BD-2 Antibody
 • STAT2 Antibody
 • Pancreatic Polypeptide (PP) ELISA Kit
 • Advanced Oxidation Protein Products (AOPP) ELISA Kit
 • Chemerin ELISA Kit
 • FMDV Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-AFP antibody :: Rabbit AFP Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS125057
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AFP, Polyclonal Antibody

 Also Known As   

AFP Polyclonal Antibody

 Product Synonym Names    AFP; FETA; Alpha fetoglobulin; alpha fetoprotein; Alpha 1 fetoprotein; HPAFP
 Product Gene Name   

anti-AFP antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    104150
 3D Structure    ModBase 3D Structure for P02771
Section Bar
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
Section Bar
 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
Section Bar
 Species    Human
 Route    Synthetic Peptide
 Immunogen    C term -peptide of human AFP
 Calculated Molecular Weight    65kDa
Section Bar
 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-AFP antibody

   Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and beta-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and beta-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).
 Product Categories/Family for anti-AFP antibody    Polyclonal
 Applications Tested/Suitable for anti-AFP antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

 Application Notes for anti-AFP antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Section Bar
 Western Blot (WB) of anti-AFP antibody    Western blot analysis of extracts of various cell lines, using AFP antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
anti-AFP antibody Western Blot (WB) (WB) image
Section Bar
 Immunofluorescence (IF) of anti-AFP antibody    Immunofluorescence analysis of HeLa cells using AFP antibody. Blue: DAPI for nuclear staining.
anti-AFP antibody Immunofluorescence (IF) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.
 NCBI GI #    120042
 NCBI GeneID    174
 NCBI Accession #    P02771.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P02771 [Other Products]
 UniProt Secondary Accession #    B2RBU3 [Other Products]
 UniProt Related Accession #    P02771 [Other Products]
 Molecular Weight    609
Table BarTOPTable Bar
 NCBI Official Full Name    Alpha-fetoprotein
 NCBI Official Synonym Full Names    alpha-fetoprotein
 NCBI Official Symbol    AFP [Similar Products]
 NCBI Official Synonym Symbols   
AFPD; FETA; HPAFP
[Similar Products]
 NCBI Protein Information    alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
 UniProt Protein Name    Alpha-fetoprotein
 UniProt Synonym Protein Names   
Alpha-1-fetoprotein; Alpha-fetoglobulin
 Protein Family    AFP
 UniProt Gene Name    AFP [Similar Products]
 UniProt Synonym Gene Names    HPAFP [Similar Products]
 UniProt Entry Name    FETA_HUMAN
Section Bar
 NCBI Summary for AFP    This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for AFP    AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
Table BarTOPTable Bar
 Research Articles on AFP    1. Hepatocellular carcinoma classification based on Gd-EOB-DTPA-enhanced MRI and serum AFP levels predicted overall survival
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-AFP antibodyPathways associated with anti-AFP antibody
 Reference Product  PubMed Publications
 JUN antibody  >939 publications with AFP and JUN
 GPC3 antibody  >69 publications with AFP and GPC3
 IGFBP1 antibody  >17 publications with AFP and IGFBP1
 FOXA1 antibody  >12 publications with AFP and FOXA1
 TP53 antibody  >8 publications with AFP and TP53
 FOXA3 antibody  >3 publications with AFP and FOXA3
 AHSG antibody  >1 publications with AFP and AHSG
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with anti-AFP antibodyOrgans/Tissues associated with anti-AFP antibody
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3983 publications with AFP and Liver Diseases
 Liver Neoplasms Antibodies  >3867 publications with AFP and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3628 publications with AFP and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >1258 publications with AFP and Nervous System Diseases
 Liver Cirrhosis Antibodies  >1114 publications with AFP and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >543 publications with AFP and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >438 publications with AFP and Neoplasms, Experimental
 Disease Models, Animal Antibodies  >394 publications with AFP and Disease Models, Animal
 Liver Neoplasms, Experimental Antibodies  >374 publications with AFP and Liver Neoplasms, Experimental
 Lung Neoplasms Antibodies  >345 publications with AFP and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4839 publications with AFP and Liver
 Blood Antibodies  >3664 publications with AFP and Blood
 Vascular Antibodies  >584 publications with AFP and Vascular
 Lung Antibodies  >392 publications with AFP and Lung
 Brain Antibodies  >324 publications with AFP and Brain
 Embryonic Tissue Antibodies  >289 publications with AFP and Embryonic Tissue
 Muscle Antibodies  >209 publications with AFP and Muscle
 Kidney Antibodies  >175 publications with AFP and Kidney
 Placenta Antibodies  >164 publications with AFP and Placenta
 Heart Antibodies  >101 publications with AFP and Heart
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions