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anti-AKT1 antibody :: Rabbit AKT Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8241684
Unit / Price
0.03 mL  /  $165 +1 FREE 8GB USB
0.1 mL  /  $245 +1 FREE 8GB USB
0.2 mL  /  $350 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AKT (AKT1), Polyclonal Antibody

 Also Known As   

Anti-AKT (pS246) Antibody

 Product Synonym Names    PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
 Product Gene Name   

anti-AKT1 antibody

[Similar Products]
 Antibody/Peptide Pairs    AKT peptide (MBS8244008) is used for blocking the activity of AKT antibody (MBS8241684)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114480
 3D Structure    ModBase 3D Structure for P31749
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Bovine, Chicken, Zebrafish
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 Specificity    Recognizes endogenous levels of AKT (pS246) protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT. The exact sequence is proprietary.
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 Preparation and Storage    Shipped and store at 4 degree C for one year. Do not freeze.
 Other Notes    Small volumes of anti-AKT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-AKT1 antibody

   Rabbit polyclonal antibody to AKT (pS246)
 Applications Tested/Suitable for anti-AKT1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

 Application Notes for anti-AKT1 antibody    Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
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 Western Blot (WB) of anti-AKT1 antibody    Western blot analysis of AKT (pS246) expression in HepG2 (A), K562 (B), NIH3T3 (C) whole cell lysates.
anti-AKT1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-AKT1 antibody    Immunohistochemical analysis of AKT (pS246) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-AKT1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-AKT1 antibody    Immunofluorescent analysis of AKT (pS246) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-AKT1 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product.
 NCBI GI #    60391226
 NCBI GeneID    207
 NCBI Accession #    P31749.2 [Other Products]
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 UniProt Primary Accession #    P31749 [Other Products]
 UniProt Secondary Accession #    Q9BWB6; B2RAM5; B7Z5R1 [Other Products]
 UniProt Related Accession #    P31749 [Other Products]
 Molecular Weight    48,347 Da
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 NCBI Official Full Name    RAC-alpha serine/threonine-protein kinase
 NCBI Official Synonym Full Names    v-akt murine thymoma viral oncogene homolog 1
 NCBI Official Symbol    AKT1 [Similar Products]
 NCBI Official Synonym Symbols   
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
 Protein Family    AKT-interacting protein
 UniProt Gene Name    AKT1 [Similar Products]
 UniProt Synonym Gene Names    PKB; RAC; PKB; PKB alpha [Similar Products]
 UniProt Entry Name    AKT1_HUMAN
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 NCBI Summary for AKT1    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for AKT1    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: AGC group; AKT family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; nucleoplasm; nucleus; plasma membrane; vesicle

Molecular Function: ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: activated T cell apoptosis; cell differentiation; cell proliferation; cellular response to insulin stimulus; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of caspase activity; negative regulation of fatty acid beta-oxidation; negative regulation of protein kinase activity; negative regulation of proteolysis; nitric oxide biosynthetic process; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; phosphorylation; platelet activation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell growth; positive regulation of cellular protein metabolic process; positive regulation of endodeoxyribonuclease activity; positive regulation of endothelial cell proliferation; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fat cell differentiation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of lipid biosynthetic process; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein import into nucleus, translocation; protein modification process; regulation of cell migration; regulation of glycogen biosynthetic process; regulation of mRNA stability; regulation of nitric-oxide synthase activity; regulation of phosphoinositide 3-kinase cascade; response to heat; response to oxidative stress; signal transduction; T cell costimulation

Disease: Breast Cancer; Colorectal Cancer; Cowden Syndrome 6; Ovarian Cancer; Proteus Syndrome; Schizophrenia
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 Research Articles on AKT1    1. The intracellular localization of Akt plays an important role in breast cancer stem-like cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AKT1 antibodyPathways associated with anti-AKT1 antibody
 Reference Product  PubMed Publications
 PTEN antibody  >602 publications with AKT1 and PTEN
 MTOR antibody  >535 publications with AKT1 and MTOR
 GSK3B antibody  >155 publications with AKT1 and GSK3B
 FOXO1 antibody  >152 publications with AKT1 and FOXO1
 NOS3 antibody  >129 publications with AKT1 and NOS3
 FOXO3 antibody  >73 publications with AKT1 and FOXO3
 MDM2 antibody  >56 publications with AKT1 and MDM2
 ILK antibody  >37 publications with AKT1 and ILK
 RICTOR antibody  >29 publications with AKT1 and RICTOR
 HSP90AA1 antibody  >5 publications with AKT1 and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
Diseases associated with anti-AKT1 antibodyOrgans/Tissues associated with anti-AKT1 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >443 publications with AKT1 and Breast Neoplasms
 Necrosis Antibodies  >310 publications with AKT1 and Necrosis
 Lung Neoplasms Antibodies  >263 publications with AKT1 and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >207 publications with AKT1 and Prostatic Neoplasms
 Inflammation Antibodies  >204 publications with AKT1 and Inflammation
 Neoplasms, Experimental Antibodies  >199 publications with AKT1 and Neoplasms, Experimental
 Heart Diseases Antibodies  >162 publications with AKT1 and Heart Diseases
 Colorectal Neoplasms Antibodies  >150 publications with AKT1 and Colorectal Neoplasms
 Liver Diseases Antibodies  >147 publications with AKT1 and Liver Diseases
 Ovarian Neoplasms Antibodies  >143 publications with AKT1 and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >837 publications with AKT1 and Blood
 Muscle Antibodies  >721 publications with AKT1 and Muscle
 Vascular Antibodies  >713 publications with AKT1 and Vascular
 Brain Antibodies  >536 publications with AKT1 and Brain
 Lung Antibodies  >424 publications with AKT1 and Lung
 Heart Antibodies  >328 publications with AKT1 and Heart
 Liver Antibodies  >327 publications with AKT1 and Liver
 Nerve Antibodies  >265 publications with AKT1 and Nerve
 Prostate Antibodies  >253 publications with AKT1 and Prostate
 Bone Antibodies  >189 publications with AKT1 and Bone
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