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anti-AKT1 + AKT2 + AKT3 antibody :: Rabbit AKT1 + AKT2 + AKT3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS242291
Unit / Price
0.05 mL  /  $495 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AKT1 + AKT2 + AKT3 (AKT1 + AKT2 + AKT3), Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3

 Product Synonym Names    Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) IHC-plus; Human AKT1 + AKT2 + AKT3
 Product Gene Name   

anti-AKT1 + AKT2 + AKT3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Chicken, Human, Mouse, Rat
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 Specificity    Synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
 Purity/Purification    Delipidated and defibrinated
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
 Concentration    75 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human AKT (AKT1/AKT2/AKT3).
 Immunogen Type    Synthetic peptide
 Immunogen    Synthetic peptide from human AKT (AKT1/AKT2/AKT3).
 Antigen Modification    C-Terminus
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 Preparation and Storage    Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
 Other Notes    Small volumes of anti-AKT1 + AKT2 + AKT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-AKT1 + AKT2 + AKT3 antibody   

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

 Application Notes for anti-AKT1 + AKT2 + AKT3 antibody    ELISA (1:2000 - 1:10000), IF (1:100 - 1:1000), IHC-P (1:100), IP, WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-AKT1 + AKT2 + AKT3 antibody    Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
anti-AKT1 + AKT2 + AKT3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-AKT1 + AKT2 + AKT3 antibody    Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
anti-AKT1 + AKT2 + AKT3 antibody Immunofluorescence (IF) image
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 Immunocytochemistry (ICC) of anti-AKT1 + AKT2 + AKT3 antibody    Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red conjugated rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
anti-AKT1 + AKT2 + AKT3 antibody Immunocytochemistry (ICC) (ICC) image
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 Western Blot (WB) of anti-AKT1 + AKT2 + AKT3 antibody    Western Blot. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot.
anti-AKT1 + AKT2 + AKT3 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-AKT1 + AKT2 + AKT3 antibody    Western blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG at 1:10000 preceded color development using Pierce Chemical's SuperSignal substrate. Block: MOPS buffer overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.
anti-AKT1 + AKT2 + AKT3 antibody Western Blot (WB) (WB) image
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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