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anti-AKT1 antibody :: Rabbit anti-Mouse, Rat AKT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS221849
Unit / Price
0.1 mg  /  $320 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AKT1, Polyclonal Antibody

 Also Known As   

RABBIT ANTI AKT1 (N-TERMINAL)

 Product Gene Name   

anti-AKT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114480
 3D Structure    ModBase 3D Structure for P31749
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse, Rat
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 Specificity    This item recognises the N-terminal region of Akt1, a 48kDa protein kinase forming part of the PI3K-Akt signalling pathway, known to regulate cell growth, proliferation and survival. The PI3K-Akt1 pathway is negatively regulated by the tumour suppressor PTEN.

Excessive Akt1 activation can lead to the formation of malignant tumours, and has been associated with prostate cancer progression.
 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.02% Sodium Azide
 Immunogen    A 16 amino acid peptide located near the human Akt1 amino-terminus.
Buffer Solution
 Antiserum Preparation    Antisera to human Akt1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-AKT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-AKT1 antibody

   MBS221849 recognises the N-terminal region of Akt1, a 48kDa protein kinase forming part of the PI3K-Akt signalling pathway, known to regulate cell growth, proliferation and survival. The PI3K-Akt1 pathway is negatively regulated by the tumour suppressor PTEN. Excessive Akt1 activation can lead to the formation of malignant tumours, and has been associated with prostate cancer progression.
 Applications Tested/Suitable for anti-AKT1 antibody   

Western Blot (WB)

 Application Notes for anti-AKT1 antibody    Western Blot: This item detects a band of approximately 48 kDa in human liver cell lysates.
Western Blotting: Maximum Dilution: 1.0ug/ml
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 Testing Data #1 of anti-AKT1 antibody    Western blot analysis of human liver cell lysate probed with Rabbit anti Human Akt1 (MBS221849) at 1(A) and 2(B) ug/ml
anti-AKT1 antibody Testing Data #1 image
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 Testing Data #2 of anti-AKT1 antibody    Immunohistochemical staining of human liver with Rabbit anti Human Akt1 (MBS221849)
anti-AKT1 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for AKT1. It may not necessarily be applicable to this product.
 NCBI GI #    62241013
 NCBI GeneID    207
 NCBI Accession #    NP_001014431.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001014431.1 [Other Products]
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 UniProt Primary Accession #    P31749 [Other Products]
 UniProt Secondary Accession #    Q9BWB6; B2RAM5; B7Z5R1 [Other Products]
 UniProt Related Accession #    P31749 [Other Products]
 Molecular Weight    48,347 Da
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 NCBI Official Full Name    RAC-alpha serine/threonine-protein kinase
 NCBI Official Synonym Full Names    v-akt murine thymoma viral oncogene homolog 1
 NCBI Official Symbol    AKT1 [Similar Products]
 NCBI Official Synonym Symbols   
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase; AKT1m; PKB alpha; RAC-PK-alpha; protein kinase B alpha; proto-oncogene c-Akt; rac protein kinase alpha
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
 Protein Family    Potassium channel
 UniProt Gene Name    AKT1 [Similar Products]
 UniProt Synonym Gene Names    PKB; RAC; PKB; PKB alpha [Similar Products]
 UniProt Entry Name    AKT1_HUMAN
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 NCBI Summary for AKT1    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for AKT1    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: nucleoplasm; microtubule cytoskeleton; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; protein amino acid phosphorylation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; regulation of cell migration; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; striated muscle cell differentiation; positive regulation of fat cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; response to oxidative stress; regulation of nitric-oxide synthase activity; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; negative regulation of cell size; glucose transport; negative regulation of caspase activity; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; inflammatory response; cell differentiation; activated T cell apoptosis; aging; epidermal growth factor receptor signaling pathway; negative regulation of proteolysis; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cell proliferation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of vasoconstriction; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; hyaluronan metabolic process

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
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 Research Articles on AKT1    1. High AKT is associated with hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AKT1 antibodyPathways associated with anti-AKT1 antibody
 Reference Product  PubMed Publications
 PTEN antibody  >519 publications with AKT1 and PTEN
 MTOR antibody  >436 publications with AKT1 and MTOR
 FOXO1 antibody  >131 publications with AKT1 and FOXO1
 MDM2 antibody  >51 publications with AKT1 and MDM2
 TSC2 antibody  >39 publications with AKT1 and TSC2
 PIK3CB antibody  >19 publications with AKT1 and PIK3CB
 TERT antibody  >14 publications with AKT1 and TERT
 GSK3B antibody  >9 publications with AKT1 and GSK3B
 UBC antibody  >6 publications with AKT1 and UBC
 HSP90AA1 antibody  >3 publications with AKT1 and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
Diseases associated with anti-AKT1 antibodyOrgans/Tissues associated with anti-AKT1 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >408 publications with AKT1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >352 publications with AKT1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >306 publications with AKT1 and Nervous System Diseases
 Necrosis Antibodies  >298 publications with AKT1 and Necrosis
 Lung Neoplasms Antibodies  >237 publications with AKT1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >198 publications with AKT1 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >197 publications with AKT1 and Prostatic Neoplasms
 Inflammation Antibodies  >181 publications with AKT1 and Inflammation
 Heart Diseases Antibodies  >151 publications with AKT1 and Heart Diseases
 Colorectal Neoplasms Antibodies  >139 publications with AKT1 and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >791 publications with AKT1 and Blood
 Muscle Antibodies  >684 publications with AKT1 and Muscle
 Vascular Antibodies  >676 publications with AKT1 and Vascular
 Brain Antibodies  >488 publications with AKT1 and Brain
 Lung Antibodies  >362 publications with AKT1 and Lung
 Heart Antibodies  >307 publications with AKT1 and Heart
 Liver Antibodies  >302 publications with AKT1 and Liver
 Nerve Antibodies  >246 publications with AKT1 and Nerve
 Prostate Antibodies  >241 publications with AKT1 and Prostate
 Kidney Antibodies  >173 publications with AKT1 and Kidney
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