Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow ALK rightarrow Polyclonal ALK  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Rattin Antibody
 • leptin ELISA Kit
 • MSX1 Antibody
 • Rad9 Antibody
 • 40S ribosomal protein S2 (RPS2) ELISA Kit
 • G 418 sulfate Biochemical
 • Neutrophil collagenase (MMP8) Recombinant Protein
 • phosphoglycerate mutase family member 5 (PGAM5) ELISA Kit
 • Fanconi anemia D2 (FANCD2) Antibody
 • fumarylacetoacetate hydrolase (fumarylacetoacetase) (FAH) ELISA Kit
 • Erythropoietin (EPO) ELISA Kit
 • Aggrecan (AGC) ELISA Kit
 • 10-4-2013 Antibody
 • Lysine decarboxylase,constitutive (ldcC) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-ALK antibody :: Rabbit anti-Human, Mouse ALK Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9401182
Western Blot (WB)
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ALK, Polyclonal Antibody

 Also Known As   

ALK (Phospho-Tyr1507) Antibody

 Product Synonym Names    kinase ALK; Anaplastic lymphoma kinase; CD246; ALK tyrosine kinase receptor precursor
 Product Gene Name   

anti-ALK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    105590
 3D Structure    ModBase 3D Structure for Q9UM73
Section Bar
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse
Section Bar
 Specificity    The antibody detects endogenous level of ALK only when phosphorylated at tyrosine 1507.
 Purity/Purification    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
Section Bar
 Immunogen Type    Peptide-KLH
 Immunogen Description    Peptide sequence around phosphorylation site of Tyrosine 1507 (P-T-Y(p)-G-S) derived from Human ALK.
 Modification    Phospho-Tyr1507
 Target Name    ALK
Section Bar
 Preparation and Storage    Store at -20 degree C/1 year
 Other Notes    Small volumes of anti-ALK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-ALK antibody

   Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
 Product Categories/Family for anti-ALK antibody    Phospho-specific Ab
 Applications Tested/Suitable for anti-ALK antibody   

Western Blot (WB)

 Application Notes for anti-ALK antibody    Western blotting: 1:500~1:1000
Section Bar
 Western Blot (WB) of anti-ALK antibody    Western blot analysis of extracts from COS7 cells treated with Anisomycin using Phospho-ALK (Tyr1507) antibody.The lane on the right is treated with the antigen-specific peptide.
anti-ALK antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for ALK. It may not necessarily be applicable to this product.
 NCBI GI #    29029632
 NCBI GeneID    238
 NCBI Accession #    NP_004295.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004304.4 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9UM73 [Other Products]
 UniProt Secondary Accession #    Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6 [Other Products]
 UniProt Related Accession #    Q9UM73 [Other Products]
 Molecular Weight    176,442 Da
Table BarTOPTable Bar
 NCBI Official Full Name    ALK tyrosine kinase receptor
 NCBI Official Synonym Full Names    anaplastic lymphoma receptor tyrosine kinase
 NCBI Official Symbol    ALK [Similar Products]
 NCBI Official Synonym Symbols   
CD246; NBLST3
[Similar Products]
 NCBI Protein Information    ALK tyrosine kinase receptor
 UniProt Protein Name    ALK tyrosine kinase receptor
 UniProt Synonym Protein Names   
Anaplastic lymphoma kinase; CD_antigen: CD246
 Protein Family    Alkylsulfatase
 UniProt Gene Name    ALK [Similar Products]
 UniProt Entry Name    ALK_HUMAN
Section Bar
 NCBI Summary for ALK    This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Section Bar
 UniProt Comments for ALK    ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; Oncoprotein; TK group; Alk family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: protein complex; integral to plasma membrane

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor

Disease: Neuroblastoma, Susceptibility To, 3
Table BarTOPTable Bar
 Research Articles on ALK    1. study identified a novel V1180L gatekeeper mutation from the cell line model and a second novel I1171T mutation from the non-small cell lung cancer patient who developed resistance to alectinib; both ALK mutations conferred resistance to alectinib as well as to crizotinib, but were sensitive to ceritinib and other next-generation ALK-TKIs
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-ALK antibodyPathways associated with anti-ALK antibody
 Reference Product  PubMed Publications
 EML4 antibody  >567 publications with ALK and EML4
 TNFRSF8 antibody  >190 publications with ALK and TNFRSF8
 STAT3 antibody  >106 publications with ALK and STAT3
 PIK3CA antibody  >97 publications with ALK and PIK3CA
 MYC antibody  >58 publications with ALK and MYC
 PTN antibody  >25 publications with ALK and PTN
 NPM1 antibody  >18 publications with ALK and NPM1
 ZC3HC1 antibody  >5 publications with ALK and ZC3HC1
 SHC1 antibody  >4 publications with ALK and SHC1
 Products by Pathway  Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
Diseases associated with anti-ALK antibodyOrgans/Tissues associated with anti-ALK antibody
 Disease Name  Pubmed Publications
 Lymphoma, Large-Cell, Anaplastic Antibodies  >1133 publications with ALK and Lymphoma, Large-Cell, Anaplastic
 Respiratory Tract Diseases Antibodies  >1122 publications with ALK and Respiratory Tract Diseases
 Lung Diseases Antibodies  >1047 publications with ALK and Lung Diseases
 Carcinoma, Non-Small-Cell Lung Antibodies  >680 publications with ALK and Carcinoma, Non-Small-Cell Lung
 Lymphoma, Large B-Cell, Diffuse Antibodies  >349 publications with ALK and Lymphoma, Large B-Cell, Diffuse
 Chromosome Aberrations Antibodies  >344 publications with ALK and Chromosome Aberrations
 Neuroblastoma Antibodies  >192 publications with ALK and Neuroblastoma
 Neoplasms, Experimental Antibodies  >131 publications with ALK and Neoplasms, Experimental
 Inflammation Antibodies  >131 publications with ALK and Inflammation
 Cell Transformation, Neoplastic Antibodies  >119 publications with ALK and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >725 publications with ALK and Blood
 Muscle Antibodies  >284 publications with ALK and Muscle
 Brain Antibodies  >265 publications with ALK and Brain
 Liver Antibodies  >236 publications with ALK and Liver
 Connective Tissue Antibodies  >43 publications with ALK and Connective Tissue
 Stomach Antibodies  >31 publications with ALK and Stomach
 Testis Antibodies  >23 publications with ALK and Testis
 Trachea Antibodies  >4 publications with ALK and Trachea
 Uncharacterized Tissue Antibodies  >1 publications with ALK and Uncharacterized Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions