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anti-Prkab2 antibody :: Rabbit AMP Activated Protein Kinase beta1,2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS618239 anti-Prkab2 antibody
Unit / Price
0.1 mg  /  $745 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AMP Activated Protein Kinase beta1,2 (Prkab2), Polyclonal Antibody

 Also Known As   

AMP Activated Protein Kinase beta1,2 (AMPK- b1,2)

 Product Synonym Names    Anti -AMP Activated Protein Kinase beta1,2 (AMPK- b1,2)
 Product Gene Name   

anti-Prkab2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_005101.2 (192498430..192510624). Location: 2q34
 3D Structure    ModBase 3D Structure for Q9QZH4
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Recognizes human AMPK- b1,2. Species Crossreactivity
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.4 0.1% BSA, 0.1% sodium azide, 40% glycerol.
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 Immunogen    A 15-aa peptide sequence mapping near the C-terminus of human AMPK-b1/2 (1) was synthesized, coupled to KLH.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-Prkab2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Prkab2 antibody

   In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria, catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, is the rate limiting step of mitochondrial fatty acid ?-oxidation. Activities of ACC-1, ACC-2 and other key proteins of carbohydrate and fat metabolism are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). AMPK switches-off biosynthetic processes when ATP levels are depleted and AMP rises in response to fuel deficiency and treatments like heat shock, ischaemia and exercise. A defect in AMPK switch leads to insulin resistance, dyslipidemia, ketosis resistance and other metabolic derangements in Type 2 diabetes. AMPK also regulates cholesterol biosynthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA resuctase. It also appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathway when ATP levels are depleted and when 5-AMP rises in response to fuel limitation and or hypoxia.
 Product Categories/Family for anti-Prkab2 antibody    Antibodies; Abs to Protein Kinases
 Applications Tested/Suitable for anti-Prkab2 antibody   

ELISA (EL/EIA), Western Blot (WB)

 Application Notes for anti-Prkab2 antibody    Suitable for use in ELISA, Western Blot.
Dilution: Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
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NCBI/Uniprot data below describe general gene information for Prkab2. It may not necessarily be applicable to this product.
 NCBI GI #    6013219
 NCBI GeneID    64562
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 UniProt Primary Accession #    Q9QZH4 [Other Products]
 UniProt Related Accession #    Q6AYZ8; Q9QZH4 [Other Products]
 Molecular Weight    30,227 Da [Similar Products]
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 NCBI Official Full Name    AMP-activated protein kinase beta-2 regulatory subunit
 NCBI Official Synonym Full Names    protein kinase, AMP-activated, beta 2 non-catalytic subunit
 NCBI Official Symbol    Prkab2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase subunit beta-2; AMPK beta-2 chain; AMPK subunit beta-2; AMP-activated protein kinase beta-2 regulatory subunit
 UniProt Protein Name    5'-AMP-activated protein kinase subunit beta-2
 Protein Family    5'-AMP-activated protein kinase
 UniProt Gene Name    Prkab2 [Similar Products]
 UniProt Entry Name    AAKB2_RAT
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 NCBI Summary for Prkab2    a component of the AMPK alpha2beta2gamma1 complex which phosphorylates glycogen synthase [RGD]
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 UniProt Comments for Prkab2    AMPKB2: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity.

Protein type: Protein kinase, regulatory subunit; Autophagy

Cellular Component: nucleoplasm; cytoplasm; apical plasma membrane; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; identical protein binding; protein kinase binding

Biological Process: signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; fatty acid biosynthetic process
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 Research Articles on Prkab2    1. Results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Prkab2 antibodyDiseases associated with anti-Prkab2 antibody
 Products by Pathway  Pathway Diagram
 AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway antibodies  AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 Import Of Palmitoyl-CoA Into The Mitochondrial Matrix Pathway antibodies  Import Of Palmitoyl-CoA Into The Mitochondrial Matrix Pathway Diagram
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >4 publications with Prkab2 and Diabetes Mellitus, Type 2
 Obesity Antibodies  >1 publications with Prkab2 and Obesity
 Cell Transformation, Neoplastic Antibodies  >1 publications with Prkab2 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >1 publications with Prkab2 and Kidney Diseases
 Insulin Resistance Antibodies  >1 publications with Prkab2 and Insulin Resistance
 Heart Diseases Antibodies  >1 publications with Prkab2 and Heart Diseases
 Cardiomyopathies Antibodies  >1 publications with Prkab2 and Cardiomyopathies
Organs/Tissues associated with anti-Prkab2 antibody
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >3 publications with Prkab2 and Heart
 Lung Antibodies  >1 publications with Prkab2 and Lung
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