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anti-AMPK1 antibody :: Rabbit AMPK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS856135
Unit / Price
0.1 mg  /  $330 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AMPK1, Polyclonal Antibody

 Also Known As   

AMPK1 Antibody

 Product Gene Name   

anti-AMPK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602739
 3D Structure    ModBase 3D Structure for Q13131
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Form/Format    Lyophilized anti-AMPK1 antibody purified by peptide affinity column containing 1% BSA and 0.02% NaN3
 Concentration    100ug/200ul (lot specific)
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 Source Note    Rabbit polyclonal AMPK1(1) antibody was raised against a 15 amino acid peptide from near the intermediate residues of human AMPK1 protein.
 Reactivity Note    Not yet tested in other species.
 Pathway    5'-AMP-activated protein kinase catalytic subunit alpha-1
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 Preparation and Storage    Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at -20 degree C after reconstitution. Avoid repeated freeze/defrost cycles.
 Other Notes    Small volumes of anti-AMPK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-AMPK1 antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-AMPK1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-AMPK1 antibody    Peptide ELISA titer: 1:40,000-1:80,000
Western blot analysis: 1:200-500
Immunohistochemistry: 1:50-200
Optimal dilutions/concentrations should be determined by the end user.
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 Testing Data of anti-AMPK1 antibody    anti-AMPK1 antibody Testing Data image
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 Testing Data #2 of anti-AMPK1 antibody    anti-AMPK1 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for AMPK1. It may not necessarily be applicable to this product.
 NCBI GI #    94557301
 NCBI GeneID    5562
 NCBI Accession #    NP_006242.5 [Other Products]
 NCBI GenBank Nucleotide #    NM_006251.5 [Other Products]
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 UniProt Primary Accession #    Q13131 [Other Products]
 UniProt Secondary Accession #    O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1 [Other Products]
 UniProt Related Accession #    Q13131 [Other Products]
 Molecular Weight    65,523 Da
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 NCBI Official Full Name    5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
 NCBI Official Synonym Full Names    protein kinase, AMP-activated, alpha 1 catalytic subunit
 NCBI Official Symbol    PRKAA1 [Similar Products]
 NCBI Official Synonym Symbols   
AMPK; AMPKa1
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase kinase; AMP-activated protein kinase, catalytic, alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain
 UniProt Protein Name    5'-AMP-activated protein kinase catalytic subunit alpha-1
 UniProt Synonym Protein Names   
Acetyl-CoA carboxylase kinase (EC:2.7.11.27); ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31); HMGCR kinase; Tau-protein kinase PRKAA1 (EC:2.7.11.26)
 UniProt Gene Name    PRKAA1 [Similar Products]
 UniProt Synonym Gene Names    AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase [Similar Products]
 UniProt Entry Name    AAPK1_HUMAN
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 NCBI Summary for AMPK1    The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AMPK1    AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.26; EC 2.7.11.27; Kinase, protein; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); Autophagy; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: nucleoplasm; apical plasma membrane; cytoplasm; intracellular; cytosol; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein C-terminus binding; cAMP-dependent protein kinase activity; metal ion binding; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; protein kinase activity; protein binding; tau-protein kinase activity; chromatin binding; kinase binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: activation of MAPK activity; protein heterooligomerization; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; glucose homeostasis; signal transduction; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to gamma radiation; negative regulation of glucosylceramide biosynthetic process; cell cycle arrest; fatty acid biosynthetic process; positive regulation of autophagy; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; cold acclimation; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; response to hypoxia; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis
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 Research Articles on AMPK1    1. AMPK activators ameliorated carrageenan-induced insulin resistance via SeP inhibition.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AMPK1 antibodyPathways associated with anti-AMPK1 antibody
 Reference Product  PubMed Publications
 PRKAA2 antibody  >76 publications with AMPK1 and PRKAA2
 STK11 antibody  >19 publications with AMPK1 and STK11
 PRKAG1 antibody  >18 publications with AMPK1 and PRKAG1
 PRKAB2 antibody  >17 publications with AMPK1 and PRKAB2
 PRKAB1 antibody  >17 publications with AMPK1 and PRKAB1
 PRKAG3 antibody  >14 publications with AMPK1 and PRKAG3
 PRKAG2 antibody  >10 publications with AMPK1 and PRKAG2
 TSC2 antibody  >2 publications with AMPK1 and TSC2
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
Diseases associated with anti-AMPK1 antibodyOrgans/Tissues associated with anti-AMPK1 antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >12 publications with AMPK1 and Cardiovascular Diseases
 Fatty Liver Antibodies  >10 publications with AMPK1 and Fatty Liver
 Inflammation Antibodies  >10 publications with AMPK1 and Inflammation
 Heart Diseases Antibodies  >9 publications with AMPK1 and Heart Diseases
 Necrosis Antibodies  >7 publications with AMPK1 and Necrosis
 Insulin Resistance Antibodies  >6 publications with AMPK1 and Insulin Resistance
 Nervous System Diseases Antibodies  >5 publications with AMPK1 and Nervous System Diseases
 Liver Diseases Antibodies  >5 publications with AMPK1 and Liver Diseases
 Disease Models, Animal Antibodies  >4 publications with AMPK1 and Disease Models, Animal
 Lung Neoplasms Antibodies  >4 publications with AMPK1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >49 publications with AMPK1 and Muscle
 Blood Antibodies  >24 publications with AMPK1 and Blood
 Liver Antibodies  >21 publications with AMPK1 and Liver
 Vascular Antibodies  >18 publications with AMPK1 and Vascular
 Adipose Tissue Antibodies  >15 publications with AMPK1 and Adipose Tissue
 Lung Antibodies  >11 publications with AMPK1 and Lung
 Brain Antibodies  >11 publications with AMPK1 and Brain
 Connective Tissue Antibodies  >9 publications with AMPK1 and Connective Tissue
 Pancreas Antibodies  >7 publications with AMPK1 and Pancreas
 Nerve Antibodies  >6 publications with AMPK1 and Nerve
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