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anti-ANAPC1 antibody :: Rabbit anti-Human ANAPC1 / APC1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240232
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ANAPC1 / APC1, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human ANAPC1 / APC1

 Product Synonym Names    Anti-ANAPC1 / APC1 Antibody (phospho-Ser377) IHC-plus; ANAPC1; APC1; Cyclosome subunit 1; MCPR; Mitotic checkpoint regulator; TSG24; Human ANAPC1; APC1
 Product Gene Name   

anti-ANAPC1 antibody

[Similar Products]
 Product Synonym Gene Name    APC1 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    608473
 3D Structure    ModBase 3D Structure for Q9H1A4
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Amino acids 373-382 of Human Apc1 protein.
 Purity/Purification    Immunoaffinity Purified
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human ANAPC1.
 Immunogen Type    Synthetic peptide
 Immunogen    ANAPC1 / APC1 antibody was raised against synthetic peptide from human ANAPC1.
 Antigen Modification    pSer377
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 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-ANAPC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ANAPC1 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-ANAPC1 antibody    ELISA (1:2000 - 1:10000), IHC-P (10 ug/ml), WB (1:200 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
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 Immunohistochemistry (IHC) of anti-ANAPC1 antibody    Anti-ANAPC1 antibody IHC of human pancreatic carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-ANAPC1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-ANAPC1 antibody    Anti-APC1 pS377 Antibody - Western Blot. Western blot of Affinity Purified anti-APC1 pS377 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
anti-ANAPC1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ANAPC1. It may not necessarily be applicable to this product.
 NCBI GI #    12056971
 NCBI GeneID    64682
 NCBI Accession #    NP_073153.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022662.3 [Other Products]
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 UniProt Primary Accession #    Q9H1A4 [Other Products]
 UniProt Secondary Accession #    Q2M3H8; Q9BSE6; Q9H8D0 [Other Products]
 UniProt Related Accession #    Q9H1A4 [Other Products]
 Molecular Weight    216,500 Da
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 NCBI Official Full Name    anaphase-promoting complex subunit 1
 NCBI Official Synonym Full Names    anaphase promoting complex subunit 1
 NCBI Official Symbol    ANAPC1 [Similar Products]
 NCBI Official Synonym Symbols   
APC1; MCPR; TSG24
[Similar Products]
 NCBI Protein Information    anaphase-promoting complex subunit 1; cyclosome subunit 1; mitotic checkpoint regulator; testis-specific gene 24 protein; anaphase-promoting complex 1 (meiotic checkpoint regulator)
 UniProt Protein Name    Anaphase-promoting complex subunit 1
 UniProt Synonym Protein Names   
Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein
 UniProt Gene Name    ANAPC1 [Similar Products]
 UniProt Synonym Gene Names    TSG24; APC1 [Similar Products]
 UniProt Entry Name    APC1_HUMAN
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 NCBI Summary for ANAPC1    This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]
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 UniProt Comments for ANAPC1    APC1: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC1 family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
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 Research Articles on ANAPC1    1. Data indicate that regulation of Rad17 turnover is through the Cdh1/anaphase-promoting complex pathway in breast cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ANAPC1 antibodyPathways associated with anti-ANAPC1 antibody
 Reference Product  PubMed Publications
 ANAPC2 antibody  >8 publications with ANAPC1 and ANAPC2
 CDC16 antibody  >6 publications with ANAPC1 and CDC16
 CDC23 antibody  >6 publications with ANAPC1 and CDC23
 ANAPC4 antibody  >6 publications with ANAPC1 and ANAPC4
 CDC27 antibody  >6 publications with ANAPC1 and CDC27
 ANAPC5 antibody  >5 publications with ANAPC1 and ANAPC5
 ANAPC7 antibody  >3 publications with ANAPC1 and ANAPC7
 CDC20 antibody  >3 publications with ANAPC1 and CDC20
 PTTG1 antibody  >1 publications with ANAPC1 and PTTG1
 CCNB1 antibody  >1 publications with ANAPC1 and CCNB1
 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Diseases associated with anti-ANAPC1 antibodyOrgans/Tissues associated with anti-ANAPC1 antibody
 Disease Name  Pubmed Publications
 Atrophy Antibodies  >1 publications with ANAPC1 and Atrophy
 Adenocarcinoma Antibodies  >1 publications with ANAPC1 and Adenocarcinoma
 Hyperplasia Antibodies  >1 publications with ANAPC1 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with ANAPC1 and Lung
 Skin Antibodies  >1 publications with ANAPC1 and Skin
 Kidney Antibodies  >1 publications with ANAPC1 and Kidney
 Uterus Antibodies  >1 publications with ANAPC1 and Uterus
 Stomach Antibodies  >1 publications with ANAPC1 and Stomach
 Intestine Antibodies  >1 publications with ANAPC1 and Intestine
 Brain Antibodies  >1 publications with ANAPC1 and Brain
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