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anti-ANAPC10 antibody :: Rabbit anti-Human ANAPC10 / APC10 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240619
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ANAPC10 / APC10, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human ANAPC10 / APC10

 Product Synonym Names    Anti-ANAPC10 / APC10 Antibody (aa10-20) IHC-plus; ANAPC10; Cyclosome subunit 10; DKFZP564L0562; APC10; DOC1; Human ANAPC10; APC10
 Product Gene Name   

anti-ANAPC10 antibody

[Similar Products]
 Product Synonym Gene Name    APC10 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    613745
 3D Structure    ModBase 3D Structure for Q9UM13
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    aa 10-20 of Human APC10 (Anaphase-promoting complex or cyclosome).
 Purity/Purification    Immunoaffinity Purified
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human ANAPC10 / APC10.
 Immunogen Type    Synthetic peptide
 Immunogen    ANAPC10 / APC10 antibody was raised against synthetic peptide from human ANAPC10 / APC10.
 Antigen Modification    aa10-20
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 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-ANAPC10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ANAPC10 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-ANAPC10 antibody    ELISA, IHC-P (2.5 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
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 Immunohistochemistry (IHC) of anti-ANAPC10 antibody    Anti-ANAPC10 / APC10 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-ANAPC10 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-ANAPC10 antibody    Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kD band corresponding to human APC10 (predicted molecular weight is 21 kD). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.
anti-ANAPC10 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-ANAPC10 antibody    Anti-APC10 Antibody - Western Blot. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kD.
anti-ANAPC10 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ANAPC10. It may not necessarily be applicable to this product.
 NCBI GI #    150170706
 NCBI GeneID    10393
 NCBI Accession #    NP_055700.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_014885.4 [Other Products]
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 UniProt Primary Accession #    Q9UM13 [Other Products]
 UniProt Secondary Accession #    Q2V500; Q9UG51; Q9Y5R0; D3DNZ7 [Other Products]
 UniProt Related Accession #    Q9UM13 [Other Products]
 Molecular Weight    21,252 Da
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 NCBI Official Full Name    anaphase-promoting complex subunit 10 isoform 1
 NCBI Official Synonym Full Names    anaphase promoting complex subunit 10
 NCBI Official Symbol    ANAPC10 [Similar Products]
 NCBI Official Synonym Symbols   
DOC1; APC10
[Similar Products]
 NCBI Protein Information    anaphase-promoting complex subunit 10; cyclosome subunit 10
 UniProt Protein Name    Anaphase-promoting complex subunit 10
 UniProt Synonym Protein Names   
Cyclosome subunit 10
 UniProt Gene Name    ANAPC10 [Similar Products]
 UniProt Synonym Gene Names    APC10; APC10 [Similar Products]
 UniProt Entry Name    APC10_HUMAN
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 NCBI Summary for ANAPC10    ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011]
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 UniProt Comments for ANAPC10    APC10: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC10 family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
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 Research Articles on ANAPC10    1. Cdc20 requires APC3 and APC8 to bind and activate the APC/C when the spindle assembly checkpoint is satisfied, but only APC8 when active, and APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ANAPC10 antibodyPathways associated with anti-ANAPC10 antibody
 Reference Product  PubMed Publications
 CDC20 antibody  >7 publications with ANAPC10 and CDC20
 CDC27 antibody  >6 publications with ANAPC10 and CDC27
 ANAPC1 antibody  >5 publications with ANAPC10 and ANAPC1
 CDC16 antibody  >4 publications with ANAPC10 and CDC16
 CDC23 antibody  >3 publications with ANAPC10 and CDC23
 PTTG1 antibody  >3 publications with ANAPC10 and PTTG1
 ANAPC7 antibody  >1 publications with ANAPC10 and ANAPC7
 CDC26 antibody  >1 publications with ANAPC10 and CDC26
 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Diseases associated with anti-ANAPC10 antibodyOrgans/Tissues associated with anti-ANAPC10 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with ANAPC10 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with ANAPC10 and Brain Diseases
 Neurobehavioral Manifestations Antibodies  >1 publications with ANAPC10 and Neurobehavioral Manifestations
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with ANAPC10 and Kidney
 Vascular Antibodies  >1 publications with ANAPC10 and Vascular
 Bone Marrow Antibodies  >1 publications with ANAPC10 and Bone Marrow
 Testis Antibodies  >1 publications with ANAPC10 and Testis
 Brain Antibodies  >1 publications with ANAPC10 and Brain
 Liver Antibodies  >1 publications with ANAPC10 and Liver
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