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anti-APEX1 antibody :: Goat APE1/APEX1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421297
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

APE1/APEX1, Polyclonal Antibody

 Also Known As   

Goat anti-APE1/APEX1 Antibody

 Product Synonym Names    APE1; APEX1; APEX; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; a; APE1 antibody; APEX1 antibody; APEX antibody; APEX nuclease (multifunctional DNA repair enzyme) antibody; APE antibody; APX antibody; APEN antibody; HAP1 antibody; REF1 antibody; REF-1 antibody; AP lyase antibody; AP endonuclease class I antibody; apurinic/apyrimidinic exonuclease antibody; multifunctional DNA repair enzyme antibody; DNA-(apurinic or apyrimidinic site) lyase antibody; apurinic/apyrimidinic (abasic) antibody; APEX nuclease 1 endonuclease antibody; deoxyribonuclease (apurinic or apyrimidinic) antibody; apurinic/apyrimidinic (abasic) endonuclease antibody; redox factor 1 antibody; APE1 / APEX1
 Product Gene Name   

anti-APEX1 antibody

[Similar Products]
 Product Synonym Gene Name    APE1 [Similar Products]
 Antibody/Peptide Pairs    APE1 / APEX1 peptide (MBS425755) is used for blocking the activity of APE1/APEX1 antibody (MBS421297)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    PKRGKKGAVA EDGD
 OMIM    107748
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2.
 Epitope    N Terminus
 Note    Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-APEX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-APEX1 antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-APEX1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration, 4-6ug/ml.
Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3ug/ml.
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 Western Blot (WB) of anti-APEX1 antibody    (0.3ug/ml) staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-APEX1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-APEX1 antibody    (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
anti-APEX1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for APEX1. It may not necessarily be applicable to this product.
 NCBI GI #    18375501
 NCBI GeneID    328
 NCBI Accession #    NP_001632.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001641.3 [Other Products]
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 UniProt Secondary Accession #    Q969L5; Q99775 [Other Products]
 UniProt Related Accession #    P27695 [Other Products]
 Molecular Weight    35,555 Da
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 NCBI Official Full Name    DNA-(apurinic or apyrimidinic site) lyase
 NCBI Official Synonym Full Names    apurinic/apyrimidinic endodeoxyribonuclease 1
 NCBI Official Symbol    APEX1 [Similar Products]
 NCBI Official Synonym Symbols   
APE; APX; APE1; APEN; APEX; HAP1; REF1
[Similar Products]
 NCBI Protein Information    DNA-(apurinic or apyrimidinic site) lyase
 UniProt Protein Name    DNA-(apurinic or apyrimidinic site) lyase
 UniProt Synonym Protein Names   
APEX nuclease; APEN
 Protein Family    DNA-(apurinic or apyrimidinic site) lyase
 UniProt Gene Name    APEX1 [Similar Products]
 UniProt Synonym Gene Names    APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1 [Similar Products]
 UniProt Entry Name    APEX1_HUMAN
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 NCBI Summary for APEX1    Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APEX1    APE1: a multifunctional enzyme that plays a central role in the cellular response to oxidative stress including DNA repair and redox regulation of transcriptional factors. Binds DNA and RNA. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway, a 3'-5' exoribonuclease for mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules, and a DNA 3' phosphodiesterase capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. Is a loading factor for POLB onto non-incised AP sites in DNA, stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB, and involved in the DNA cleavage step of class switch recombination (CSR). Possesses reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Binds to negative calcium response elements (nCaREs). Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Is an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover. In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Interacts with SIRT1; the interaction is increased in the context of genotoxic stress. Interacts with HDAC1, HDAC2 and HDAC3; the interactions are not dependent on the APEX1 acetylation status. Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate. NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: Nuclear receptor co-regulator; EC 4.2.99.18; Lyase; DNA-binding; Nucleolus; Hydrolase; Deoxyribonuclease; Endoplasmic reticulum; DNA repair, damage; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: centrosome; cytoplasm; endoplasmic reticulum; mitochondrion; nuclear chromosome, telomeric region; nuclear speck; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; ribosome; transcription factor complex

Molecular Function: 3'-5' exonuclease activity; chromatin DNA binding; damaged DNA binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; double-stranded DNA specific exodeoxyribonuclease activity; double-stranded telomeric DNA binding; endodeoxyribonuclease activity; endonuclease activity; metal ion binding; NF-kappaB binding; oxidoreductase activity; phosphodiesterase I activity; phosphoric diester hydrolase activity; protein binding; protein complex binding; ribonuclease H activity; site-specific endodeoxyribonuclease activity, specific for altered base; transcription coactivator activity; transcription corepressor activity; uracil DNA N-glycosylase activity

Biological Process: aging; base-excision repair; base-excision repair, base-free sugar-phosphate removal; cell redox homeostasis; DNA recombination; DNA repair; negative regulation of smooth muscle cell migration; positive regulation of DNA repair; regulation of mRNA stability; regulation of transcription, DNA-dependent; response to drug; telomere maintenance; transcription, DNA-dependent
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 Research Articles on APEX1    1. Our results implicate APE1 in novel molecular interactions that regulate early stress responses elicited by microbial infections.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-APEX1 antibodyPathways associated with anti-APEX1 antibody
 Reference Product  PubMed Publications
 PCNA antibody  >29 publications with APEX1 and PCNA
 FEN1 antibody  >26 publications with APEX1 and FEN1
 SET antibody  >19 publications with APEX1 and SET
 MUTYH antibody  >17 publications with APEX1 and MUTYH
 TP53 antibody  >12 publications with APEX1 and TP53
 POLB antibody  >11 publications with APEX1 and POLB
 GZMA antibody  >5 publications with APEX1 and GZMA
 NME1 antibody  >2 publications with APEX1 and NME1
 ANP32A antibody  >2 publications with APEX1 and ANP32A
 HMGB2 antibody  >1 publications with APEX1 and HMGB2
 Products by Pathway  Pathway Diagram
 Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Displacement Of DNA Glycosylase By APEX1 Pathway antibodies  Displacement Of DNA Glycosylase By APEX1 Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 PCNA-Dependent Long Patch Base Excision Repair Pathway antibodies  PCNA-Dependent Long Patch Base Excision Repair Pathway Diagram
Diseases associated with anti-APEX1 antibodyOrgans/Tissues associated with anti-APEX1 antibody
 Disease Name  Pubmed Publications
 Genetic Predisposition to Disease Antibodies  >99 publications with APEX1 and Genetic Predisposition to Disease
 Lung Neoplasms Antibodies  >73 publications with APEX1 and Lung Neoplasms
 Nervous System Diseases Antibodies  >69 publications with APEX1 and Nervous System Diseases
 Adenocarcinoma Antibodies  >54 publications with APEX1 and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >47 publications with APEX1 and Cardiovascular Diseases
 Liver Diseases Antibodies  >32 publications with APEX1 and Liver Diseases
 Necrosis Antibodies  >31 publications with APEX1 and Necrosis
 Inflammation Antibodies  >27 publications with APEX1 and Inflammation
 Disease Progression Antibodies  >24 publications with APEX1 and Disease Progression
 Neoplasms, Experimental Antibodies  >24 publications with APEX1 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >94 publications with APEX1 and Lung
 Blood Antibodies  >93 publications with APEX1 and Blood
 Brain Antibodies  >75 publications with APEX1 and Brain
 Liver Antibodies  >60 publications with APEX1 and Liver
 Vascular Antibodies  >42 publications with APEX1 and Vascular
 Bone Antibodies  >23 publications with APEX1 and Bone
 Nerve Antibodies  >22 publications with APEX1 and Nerve
 Skin Antibodies  >20 publications with APEX1 and Skin
 Stomach Antibodies  >20 publications with APEX1 and Stomach
 Prostate Antibodies  >19 publications with APEX1 and Prostate
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