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anti-APOA4 antibody :: Goat APOA4 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420479
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

APOA4, Polyclonal Antibody

 Also Known As   

Goat anti-APOA4 Antibody

 Product Synonym Names    APOA4; apolipoprotein A-IV; MGC142154; MGC142156; APOA4 antibody; apolipoprotein A-IV antibody; MGC142154 antibody; MGC142156 antibody
 Product Gene Name   

anti-APOA4 antibody

[Similar Products]
 Antibody/Peptide Pairs    APOA4 peptide (MBS426316) is used for blocking the activity of APOA4 antibody (MBS420479)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    KEKESQDKTL SLP
 OMIM    107690
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-KEKESQDKTLSLP, from the internal region (near the C Terminus) of the protein sequence according to NP_000473.2.
 Epitope    Internal region (near the C Terminus)
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-APOA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-APOA4 antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-APOA4 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Intestine shows specific staining of cell groups in the Lamina propria. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 45.4kDa according to NP_000473.2). Recommended concentration: 0.01-0.03ug/ml.
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 Western Blot (WB) of anti-APOA4 antibody    (0.01ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-APOA4 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-APOA4 antibody    (2ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-APOA4 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for APOA4. It may not necessarily be applicable to this product.
 NCBI GI #    71773110
 NCBI GeneID    337
 NCBI Accession #    NP_000473.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000482.3 [Other Products]
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 UniProt Secondary Accession #    Q14CW8; Q6Q787; A8MSL6 [Other Products]
 UniProt Related Accession #    P06727 [Other Products]
 Molecular Weight    45,399 Da
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 NCBI Official Full Name    apolipoprotein A-IV
 NCBI Official Synonym Full Names    apolipoprotein A4
 NCBI Official Symbol    APOA4 [Similar Products]
 NCBI Protein Information    apolipoprotein A-IV
 UniProt Protein Name    Apolipoprotein A-IV
 UniProt Synonym Protein Names   
Apolipoprotein A4
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOA4 [Similar Products]
 UniProt Synonym Gene Names    Apo-AIV; ApoA-IV [Similar Products]
 UniProt Entry Name    APOA4_HUMAN
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 NCBI Summary for APOA4    Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]
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 UniProt Comments for APOA4    APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding; Cell adhesion; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: cell surface; chylomicron; cytosol; early endosome; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: antioxidant activity; cholesterol binding; cholesterol transporter activity; copper ion binding; lipid binding; lipid transporter activity; phosphatidylcholine binding; protein binding; protein homodimerization activity

Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; hydrogen peroxide catabolic process; innate immune response in mucosa; leukocyte adhesion; lipid homeostasis; lipid transport; lipoprotein biosynthetic process; lipoprotein metabolic process; multicellular organismal lipid catabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; protein-lipid complex assembly; regulation of cholesterol absorption; regulation of cholesterol transport; removal of superoxide radicals; response to lipid hydroperoxide; retinoid metabolic process; reverse cholesterol transport
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 Research Articles on APOA4    1. analysis of methylation patterns of the APOA1/C3/A4/A5 cluster that may be directly involved in the transcriptional regulation of this cluster, especially in liver
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-APOA4 antibodyOrgans/Tissues associated with anti-APOA4 antibody
 Products by Pathway  Pathway Diagram
 Amyloid Fiber Formation Pathway antibodies  Amyloid Fiber Formation Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Fat-soluble Vitamins Pathway antibodies  Metabolism Of Fat-soluble Vitamins Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >78 publications with APOA4 and Blood
 Liver Antibodies  >27 publications with APOA4 and Liver
 Intestine Antibodies  >10 publications with APOA4 and Intestine
 Pancreas Antibodies  >2 publications with APOA4 and Pancreas
 Stomach Antibodies  >1 publications with APOA4 and Stomach
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