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anti-APOD antibody :: Rabbit APOD / Apolipoprotein D Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS241904
Immunohistochemistry (IHC)
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

APOD / Apolipoprotein D, Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Polyclonal to Mouse APOD / Apolipoprotein D

 Product Synonym Names    APOD Antibody, Apo-D Antibody, Apolipoprotein D Antibody
 Product Gene Name   

anti-APOD antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    107740
 3D Structure    ModBase 3D Structure for P05090
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Synthetic peptide corresponding to N-terminal residues of mouse APOD(Apolipoprotein D precursor).
 Purity/Purification    Immunoaffinity Purified
 Form/Format    PBS, 0.01% sodium azide, 50% glycerol.
 Concentration    0.5 mg/ml (lot specific)
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 Target    Mouse APOD / Apolipoprotein D
 Target Species    Mouse
 Immunogen Description    Synthetic peptide from mouse APOD / Apolipoprotein D.
 Immunogen Type    Synthetic peptide
 Immunogen    APOD / Apolipoprotein D antibody was raised against synthetic peptide from mouse APOD / Apolipoprotein D.
 Antigen Modification    N-Terminus
 Disclaimer    Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
 Recommended Immunohistochemistry Protocol    The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
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 Preparation and Storage    Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
 Other Notes    Small volumes of anti-APOD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-APOD antibody

   Apolipoprotein D (Apo-D) is a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. It is a glycoprotein of estimated molecular weight 33 KDa.
 Applications Tested/Suitable for anti-APOD antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

 Application Notes for anti-APOD antibody    IHC-P (10 ug/ml), WB (1 ug/ml)

Usage:
Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241904 was determined to be 10 ug/ml.
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 Immunohistochemistry (IHC) of anti-APOD antibody    Anti-APOD / Apolipoprotein D antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-APOD antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for APOD. It may not necessarily be applicable to this product.
 NCBI GI #    4502163
 NCBI GeneID    347
 NCBI Accession #    NP_001638.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001647.3 [Other Products]
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 UniProt Primary Accession #    P05090 [Other Products]
 UniProt Secondary Accession #    Q6IBG6; B2R579; D3DNW6 [Other Products]
 UniProt Related Accession #    P05090 [Other Products]
 Molecular Weight    21,276 Da
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 NCBI Official Full Name    apolipoprotein D
 NCBI Official Synonym Full Names    apolipoprotein D
 NCBI Official Symbol    APOD [Similar Products]
 NCBI Protein Information    apolipoprotein D; apo-D
 UniProt Protein Name    Apolipoprotein D
 Protein Family    Apolipoprotein
 UniProt Gene Name    APOD [Similar Products]
 UniProt Synonym Gene Names    Apo-D; ApoD [Similar Products]
 UniProt Entry Name    APOD_HUMAN
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 NCBI Summary for APOD    This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]
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 UniProt Comments for APOD    APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Lipid-binding; Secreted

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extracellular space; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; dendrite; extracellular region

Molecular Function: lipid transporter activity; protein binding; cholesterol binding

Biological Process: response to drug; negative regulation of smooth muscle cell proliferation; negative regulation of focal adhesion formation; glucose metabolic process; negative regulation of protein import into nucleus; lipid transport; axon regeneration in the peripheral nervous system; response to reactive oxygen species; tissue regeneration; brain development; lipid metabolic process; response to axon injury; angiogenesis; aging
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 Research Articles on APOD    1. ApoE is located in the nucleus and on the ApoD promoter in human hepatic and glioblastoma cells lines.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-APOD antibodyPathways associated with anti-APOD antibody
 Reference Product  PubMed Publications
 LEPR antibody  >2 publications with APOD and LEPR
 Products by Pathway  Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Fatty Acids Pathway antibodies  Transport Of Fatty Acids Pathway Diagram
 Transport Of Vitamins, Nucleosides, And Related Molecules Pathway antibodies  Transport Of Vitamins, Nucleosides, And Related Molecules Pathway Diagram
Diseases associated with anti-APOD antibodyOrgans/Tissues associated with anti-APOD antibody
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >191 publications with APOD and Skin Diseases
 Breast Neoplasms Antibodies  >155 publications with APOD and Breast Neoplasms
 Carcinoma Antibodies  >124 publications with APOD and Carcinoma
 Adenocarcinoma Antibodies  >67 publications with APOD and Adenocarcinoma
 Nervous System Diseases Antibodies  >50 publications with APOD and Nervous System Diseases
 Skin Neoplasms Antibodies  >44 publications with APOD and Skin Neoplasms
 Brain Diseases Antibodies  >39 publications with APOD and Brain Diseases
 Neurodegenerative Diseases Antibodies  >27 publications with APOD and Neurodegenerative Diseases
 Cardiovascular Diseases Antibodies  >15 publications with APOD and Cardiovascular Diseases
 Metaplasia Antibodies  >15 publications with APOD and Metaplasia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >78 publications with APOD and Brain
 Skin Antibodies  >70 publications with APOD and Skin
 Nerve Antibodies  >29 publications with APOD and Nerve
 Liver Antibodies  >27 publications with APOD and Liver
 Mammary Gland Antibodies  >23 publications with APOD and Mammary Gland
 Muscle Antibodies  >21 publications with APOD and Muscle
 Prostate Antibodies  >18 publications with APOD and Prostate
 Vascular Antibodies  >17 publications with APOD and Vascular
 Lung Antibodies  >16 publications with APOD and Lung
 Lymph Node Antibodies  >14 publications with APOD and Lymph Node
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