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anti-ARFGAP3 antibody :: Rabbit anti-Human ARFGAP3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS1493385
Unit / Price
0.05 mL  /  $120 +1 FREE 8GB USB
0.1 mL  /  $160 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ARFGAP3, Polyclonal Antibody

★Popular Item★
 Also Known As   

ARFGAP3 Antibody

 Product Synonym Names    ARF GAP 3; ARFGAP3; ARFGAP1
 Product Gene Name   

anti-ARFGAP3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    612439
 3D Structure    ModBase 3D Structure for Q9NP61
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Antigen Affinity Purified
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 Storage Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Conjugate    Non-conjugated
 Immunogen    Recombinant human ADP-ribosylation factor GTPase-activating protein 3 protein (217-516aa)
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46784 / sc-46779 / sc-292277
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-ARFGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ARFGAP3 antibody

   GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
 Applications Tested/Suitable for anti-ARFGAP3 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

 Application Notes for anti-ARFGAP3 antibody    Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
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 Western Blot (WB) of anti-ARFGAP3 antibody    Western blot
All lanes: ARFGAP3 antibody at 4ug/ml
Lane 1:HepG2 whole cell lysate
Lane 2:Jurkat whole cell lysate
Lane 3:A549 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 57,52 kDa
Observed band size: 57 kDa

anti-ARFGAP3 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-ARFGAP3 antibody    Immunohistochemistry of paraffin-embedded human breast cancer using MBS1493385 at dilution 1:100
anti-ARFGAP3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-ARFGAP3 antibody    Immunohistochemistry of paraffin-embedded human liver cancer using MBS1493385 at dilution 1:100
anti-ARFGAP3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunoprecipitation (IP) of anti-ARFGAP3 antibody    Immunoprecipitating ARFGAP3 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS1493385 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2:MBS1493385(4ug)+ HepG2 whole cell lysate(500ug)
Lane 3: HepG2 whole cell lysate (20ug)

anti-ARFGAP3 antibody Immunoprecipitation (IP) (IP) image
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NCBI/Uniprot data below describe general gene information for ARFGAP3. It may not necessarily be applicable to this product.
 NCBI GI #    214829714
 NCBI GeneID    26286
 NCBI Accession #    NP_001135765.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001142293.1 [Other Products]
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 UniProt Primary Accession #    Q9NP61 [Other Products]
 UniProt Secondary Accession #    Q9BSC6; Q9H9J0; Q9NT10; Q9NUP5; Q9Y4V3; Q9Y4V4; E9PB03 [Other Products]
 UniProt Related Accession #    Q9NP61 [Other Products]
 Molecular Weight    51,990 Da
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 NCBI Official Full Name    ADP-ribosylation factor GTPase-activating protein 3 isoform 2
 NCBI Official Synonym Full Names    ADP ribosylation factor GTPase activating protein 3
 NCBI Official Symbol    ARFGAP3 [Similar Products]
 NCBI Official Synonym Symbols   
ARFGAP1
[Similar Products]
 NCBI Protein Information    ADP-ribosylation factor GTPase-activating protein 3
 UniProt Protein Name    ADP-ribosylation factor GTPase-activating protein 3
 Protein Family    ADP-ribosylation factor GTPase-activating protein
 UniProt Gene Name    ARFGAP3 [Similar Products]
 UniProt Synonym Gene Names    ARFGAP1; ARF GAP 3 [Similar Products]
 UniProt Entry Name    ARFG3_HUMAN
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 NCBI Summary for ARFGAP3    The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
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 UniProt Comments for ARFGAP3    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
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 Research Articles on ARFGAP3    1. We suggest a model in which ArfGAP3 regulates Golgi association of GGA clathrin adaptors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ARFGAP3 antibodyPathways associated with anti-ARFGAP3 antibody
 Reference Product  PubMed Publications
 ARF1 antibody  >10 publications with ARFGAP3 and ARF1
 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 COPI Mediated Transport Pathway antibodies  COPI Mediated Transport Pathway Diagram
 COPI-mediated Anterograde Transport Pathway antibodies  COPI-mediated Anterograde Transport Pathway Diagram
 ER To Golgi Anterograde Transport Pathway antibodies  ER To Golgi Anterograde Transport Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Golgi To ER Retrograde Transport Pathway antibodies  Golgi To ER Retrograde Transport Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
Organs/Tissues associated with anti-ARFGAP3 antibody
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >2 publications with ARFGAP3 and Eye
 Ovary Antibodies  >2 publications with ARFGAP3 and Ovary
 Prostate Antibodies  >2 publications with ARFGAP3 and Prostate
 Testis Antibodies  >2 publications with ARFGAP3 and Testis
 Lung Antibodies  >1 publications with ARFGAP3 and Lung
 Blood Antibodies  >1 publications with ARFGAP3 and Blood
 Liver Antibodies  >1 publications with ARFGAP3 and Liver
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