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anti-ASAH2 antibody :: Rabbit ASAH2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS151329
Western Blot (WB)
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ASAH2, Polyclonal Antibody

 Also Known As   

ASAH2 Antibody

 Product Synonym Names    ASAH2; HNAC1; BCDase; LCDase; NCDase; N-CDase; HNAC1; Neutral ceramidase; Acylsphingosine deacylase 2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
 Product Gene Name   

anti-ASAH2 antibody

[Similar Products]
 Antibody/Peptide Pairs    ASAH2 peptide (MBS153221) is used for blocking the activity of ASAH2 antibody (MBS151329)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    611202
 3D Structure    ModBase 3D Structure for Q9NR71
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    ASAH2 Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2.
 Buffer    ASAH2 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    ASAH2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ASAH2 antibody

   ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.
 Applications Tested/Suitable for anti-ASAH2 antibody   

ELISA (EIA), Western Blot (WB)

 Application Notes for anti-ASAH2 antibody    ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 mug/mL.
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 Western Blot (WB) of anti-ASAH2 antibody    Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.
anti-ASAH2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.
 NCBI GI #    221218981
 NCBI GeneID    56624
 NCBI Accession #    NP_063946 [Other Products]
 NCBI GenBank Nucleotide #    NM_019893.2 [Other Products]
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 UniProt Primary Accession #    Q9NR71 [Other Products]
 UniProt Secondary Accession #    Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6 [Other Products]
 UniProt Related Accession #    Q9NR71 [Other Products]
 Molecular Weight    81,718 Da
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 NCBI Official Full Name    neutral ceramidase isoform a
 NCBI Official Synonym Full Names    N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
 NCBI Official Symbol    ASAH2 [Similar Products]
 NCBI Official Synonym Symbols   
HNAC1; BCDase; LCDase; NCDase; N-CDase
[Similar Products]
 NCBI Protein Information    neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2
 UniProt Protein Name    Neutral ceramidase
 UniProt Synonym Protein Names   
Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidaseNeutral ceramidase soluble form
 Protein Family    Neutral ceramidase
 UniProt Gene Name    ASAH2 [Similar Products]
 UniProt Synonym Gene Names    HNAC1; N-CDase; NCDase; hCD [Similar Products]
 UniProt Entry Name    ASAH2_HUMAN
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 NCBI Summary for ASAH2    Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for ASAH2    ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.23; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; ceramide metabolic process; signal transduction
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 Research Articles on ASAH2    1. These results demonstrate for the first time that adiponectin inhibits TNFalpha-induced inflammatory response via Cav1-mediated ceramidase recruitment and activation in an AdipoR1-dependent fashion.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ASAH2 antibodyPathways associated with anti-ASAH2 antibody
 Reference Product  PubMed Publications
 SMPD1 antibody  >53 publications with ASAH2 and SMPD1
 SPHK2 antibody  >3 publications with ASAH2 and SPHK2
 SPHK1 antibody  >3 publications with ASAH2 and SPHK1
 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
 Sphingosine Biosynthesis Pathway antibodies  Sphingosine Biosynthesis Pathway Diagram
Diseases associated with anti-ASAH2 antibodyOrgans/Tissues associated with anti-ASAH2 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with ASAH2 and Necrosis
 Inflammation Antibodies  >8 publications with ASAH2 and Inflammation
 Nervous System Diseases Antibodies  >6 publications with ASAH2 and Nervous System Diseases
 Fatty Liver Antibodies  >4 publications with ASAH2 and Fatty Liver
 Disease Models, Animal Antibodies  >4 publications with ASAH2 and Disease Models, Animal
 Gastrointestinal Diseases Antibodies  >4 publications with ASAH2 and Gastrointestinal Diseases
 Cardiovascular Diseases Antibodies  >3 publications with ASAH2 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >3 publications with ASAH2 and Kidney Diseases
 Immune System Diseases Antibodies  >2 publications with ASAH2 and Immune System Diseases
 Drug-Induced Liver Injury Antibodies  >2 publications with ASAH2 and Drug-Induced Liver Injury
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >3 publications with ASAH2 and Skin
 Mixed Antibodies  >1 publications with ASAH2 and Mixed
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