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anti-ASAH2 antibody :: Rabbit anti-Human, mouse ASAH2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9210331
Western Blot (WB)
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ASAH2, Polyclonal Antibody

★Popular Item★
 Also Known As   

ASAH2 Antibody (C-term)

 Product Synonym Names    Neutral ceramidase; N-CDase; NCDase; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form; ASAH2; HNAC1
 Product Gene Name   

anti-ASAH2 antibody

[Similar Products]
 Antibody/Peptide Pairs    ASAH2 peptide (MBS9222767) is used for blocking the activity of ASAH2 antibody (MBS9210331)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    734-762
 OMIM    611202
 3D Structure    ModBase 3D Structure for Q9NR71
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human, mouse
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 Specificity    This ASAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734-762 amino acids from the C-terminal region of human ASAH2.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.5 (lot specific)
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 Antigen Type    Synthetic Peptide
 Antigen Source    HUMAN
 Function    Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
 Cellular Location    Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo
 Tissue Location    Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ASAH2 antibody

   Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze
hydrolysis of the N-acyl linkage of ceramide, a second messenger in
a variety of cellular events, to produce sphingosine. Sphingosine
exerts both mitogenic and apoptosis-inducing activities, and its
phosphorylated form functions as an intra- and intercellular second
messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed
11328816]).
 Product Categories/Family for anti-ASAH2 antibody    Metabolism; Signal Transduction
 Applications Tested/Suitable for anti-ASAH2 antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-ASAH2 antibody    WB~~1:1000
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 Western Blot (WB) of anti-ASAH2 antibody    ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).
anti-ASAH2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.
 NCBI GI #    221307475
 NCBI GeneID    56624
 NCBI Accession #    NP_001137446.1 [Other Products]
 NCBI Related Accession #    NP_063946.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001143974.1 [Other Products]
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 UniProt Primary Accession #    Q9NR71 [Other Products]
 UniProt Secondary Accession #    Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6 [Other Products]
 UniProt Related Accession #    Q9NR71 [Other Products]
 Molecular Weight    85516
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 NCBI Official Full Name    neutral ceramidase isoform b
 NCBI Official Synonym Full Names    N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
 NCBI Official Symbol    ASAH2 [Similar Products]
 NCBI Official Synonym Symbols   
HNAC1; BCDase; LCDase; NCDase; N-CDase
[Similar Products]
 NCBI Protein Information    neutral ceramidase
 UniProt Protein Name    Neutral ceramidase
 UniProt Synonym Protein Names   
Acylsphingosine deacylase 2; BCDase; LCDase; hCD
 Protein Family    Neutral ceramidase
 UniProt Gene Name    ASAH2 [Similar Products]
 UniProt Synonym Gene Names    HNAC1; N-CDase; NCDase; hCD [Similar Products]
 UniProt Entry Name    ASAH2_HUMAN
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 NCBI Summary for ASAH2    Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for ASAH2    ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.5.1.23; Hydrolase

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; signal transduction; ceramide metabolic process
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Product References and Citations for anti-ASAH2 antibody

   Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010)
Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007)
Ohlsson, L., et al. Biochimie 89(8):950-960(2007)
Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007)
Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :
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 Research Articles on ASAH2    1. These results demonstrate for the first time that adiponectin inhibits TNFalpha-induced inflammatory response via Cav1-mediated ceramidase recruitment and activation in an AdipoR1-dependent fashion.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ASAH2 antibodyPathways associated with anti-ASAH2 antibody
 Reference Product  PubMed Publications
 ASAH1 antibody  >92 publications with ASAH2 and ASAH1
 SMPD1 antibody  >53 publications with ASAH2 and SMPD1
 SPHK2 antibody  >3 publications with ASAH2 and SPHK2
 SPHK1 antibody  >3 publications with ASAH2 and SPHK1
 SMPD3 antibody  >1 publications with ASAH2 and SMPD3
 Products by Pathway  Pathway Diagram
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 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
Diseases associated with anti-ASAH2 antibodyOrgans/Tissues associated with anti-ASAH2 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with ASAH2 and Necrosis
 Inflammation Antibodies  >8 publications with ASAH2 and Inflammation
 Nervous System Diseases Antibodies  >6 publications with ASAH2 and Nervous System Diseases
 Fatty Liver Antibodies  >4 publications with ASAH2 and Fatty Liver
 Disease Models, Animal Antibodies  >4 publications with ASAH2 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >3 publications with ASAH2 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >3 publications with ASAH2 and Kidney Diseases
 Glioma Antibodies  >3 publications with ASAH2 and Glioma
 Drug-Induced Liver Injury Antibodies  >2 publications with ASAH2 and Drug-Induced Liver Injury
 Hyperplasia Antibodies  >1 publications with ASAH2 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >3 publications with ASAH2 and Skin
 Mixed Antibodies  >1 publications with ASAH2 and Mixed
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