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anti-MAP3K5 antibody :: Rabbit anti-Human ASK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150980
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ASK1 (MAP3K5), Polyclonal Antibody

 Also Known As   

ASK1 Antibody

 Product Synonym Names    ASK1; ASK1; MEKK5; MAPKKK5; ASK1; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; mitogen-activated protein kinase kinase kinase 5
 Product Gene Name   

anti-MAP3K5 antibody

[Similar Products]
 Antibody/Peptide Pairs    ASK1 peptide (MBS151786) is used for blocking the activity of ASK1 antibody (MBS150980)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602448
 3D Structure    ModBase 3D Structure for Q99683
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    ASK1 Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
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 Conjugate    Unconjugated
 Immunogen    ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. This sequence is different from that of mouse by last two amino acids.
 Buffer    ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    ASK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-MAP3K5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAP3K5 antibody

   ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
 Applications Tested/Suitable for anti-MAP3K5 antibody   

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-MAP3K5 antibody    ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 mug/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
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 Western Blot (WB) of anti-MAP3K5 antibody    Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
anti-MAP3K5 antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-MAP3K5 antibody    Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 μg/mL.
anti-MAP3K5 antibody Immunocytochemistry (ICC) (ICC) image
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 Immunofluorescence (IF) of anti-MAP3K5 antibody    Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 μg/mL.
anti-MAP3K5 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for MAP3K5. It may not necessarily be applicable to this product.
 NCBI GI #    6685617
 NCBI GeneID    4217
 NCBI Accession #    Q99683.1 [Other Products]
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 UniProt Primary Accession #    Q99683 [Other Products]
 UniProt Secondary Accession #    Q5THN3; Q99461; A6NIA0; B4DGB2 [Other Products]
 UniProt Related Accession #    Q99683 [Other Products]
 Molecular Weight    155 kDa
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 NCBI Official Full Name    Mitogen-activated protein kinase kinase kinase 5
 NCBI Official Synonym Full Names    mitogen-activated protein kinase kinase kinase 5
 NCBI Official Symbol    MAP3K5 [Similar Products]
 NCBI Official Synonym Symbols   
ASK1; MEKK5; MAPKKK5
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1
 UniProt Protein Name    Mitogen-activated protein kinase kinase kinase 5
 UniProt Synonym Protein Names   
Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
 Protein Family    Mitogen-activated protein kinase kinase kinase
 UniProt Gene Name    MAP3K5 [Similar Products]
 UniProt Synonym Gene Names    ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5 [Similar Products]
 UniProt Entry Name    M3K5_HUMAN
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 NCBI Summary for MAP3K5    Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MAP3K5    ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytosol

Molecular Function: protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; magnesium ion binding; caspase activator activity; protein kinase binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of JNK activity; activation of MAPKK activity; viral reproduction; positive regulation of apoptosis; MAPKKK cascade; positive regulation of JNK cascade; positive regulation of caspase activity; protein amino acid phosphorylation; activation of JNK activity; innate immune response; JNK cascade; induction of apoptosis by oxidative stress
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 Research Articles on MAP3K5    1. Data show that ASK1 is critical for IFN gamma-induced DAPK1 via ATF6 recruitment.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAP3K5 antibodyPathways associated with anti-MAP3K5 antibody
 Reference Product  PubMed Publications
 TRAF2 antibody  >23 publications with MAP3K5 and TRAF2
 DAXX antibody  >17 publications with MAP3K5 and DAXX
 MAP2K6 antibody  >11 publications with MAP3K5 and MAP2K6
 TXN antibody  >10 publications with MAP3K5 and TXN
 MAP2K3 antibody  >9 publications with MAP3K5 and MAP2K3
 TRAF6 antibody  >9 publications with MAP3K5 and TRAF6
 MAP2K7 antibody  >5 publications with MAP3K5 and MAP2K7
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway antibodies  HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
Diseases associated with anti-MAP3K5 antibodyOrgans/Tissues associated with anti-MAP3K5 antibody
 Disease Name  Pubmed Publications
 Death Antibodies  >135 publications with MAP3K5 and Death
 Necrosis Antibodies  >70 publications with MAP3K5 and Necrosis
 Nervous System Diseases Antibodies  >35 publications with MAP3K5 and Nervous System Diseases
 Inflammation Antibodies  >27 publications with MAP3K5 and Inflammation
 Brain Diseases Antibodies  >25 publications with MAP3K5 and Brain Diseases
 Disease Models, Animal Antibodies  >23 publications with MAP3K5 and Disease Models, Animal
 Heart Diseases Antibodies  >13 publications with MAP3K5 and Heart Diseases
 Liver Diseases Antibodies  >11 publications with MAP3K5 and Liver Diseases
 Hypertrophy Antibodies  >10 publications with MAP3K5 and Hypertrophy
 Lung Diseases Antibodies  >10 publications with MAP3K5 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with MAP3K5 and Muscle
 Brain Antibodies  >27 publications with MAP3K5 and Brain
 Heart Antibodies  >25 publications with MAP3K5 and Heart
 Liver Antibodies  >18 publications with MAP3K5 and Liver
 Kidney Antibodies  >15 publications with MAP3K5 and Kidney
 Lung Antibodies  >12 publications with MAP3K5 and Lung
 Bone Antibodies  >11 publications with MAP3K5 and Bone
 Nerve Antibodies  >11 publications with MAP3K5 and Nerve
 Embryonic Tissue Antibodies  >8 publications with MAP3K5 and Embryonic Tissue
 Prostate Antibodies  >7 publications with MAP3K5 and Prostate
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