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anti-ASK1 antibody :: Rabbit ASK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS223333
Unit / Price
0.1 mg  /  $325 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ASK1, Polyclonal Antibody

 Also Known As   

RABBIT ANTI HUMAN ASK1 (C-TERMINAL)

 Product Gene Name   

anti-ASK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602448
 3D Structure    ModBase 3D Structure for Q99683
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
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 Specificity    This item recognises human ASK1 (Apoptosis signal-regulating kinase 1) a 155kDa protein belonging to the protein kinase superfamily.

ASK1 is a serine/threonine kinase, also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.

ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 0.5 mg/ml (lot specific)
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 Perservative Stabilisers    0.02% Sodium Azide (NaN3)
 Immunogen    A peptide corresponding to amino acids near the carboxy terminus of human ASK1.
Buffer Solution
 Antiserum Preparation    Antiserum to human ASK1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-ASK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ASK1 antibody

   MBS223333 recognises human ASK1 (Apoptosis signal-regulating kinase 1) a 155kDa protein belonging to the protein kinase superfamily. ASK1 is a serine/threonine kinase, also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis. ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
 Applications Tested/Suitable for anti-ASK1 antibody   

Immunohistology Frozen, Western Blot (WB)

 Application Notes for anti-ASK1 antibody    Western Blot: This item detects a band of approximately 155kDa in SW1353 cell lysates.
Immunohistology - Frozen: Maximum Dilution: 10ug/ml
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
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 Testing Data #1 of anti-ASK1 antibody    Western Blot analysis of whole cell lysate from SW1353 chondrosarcoma cell line probed with Rabbit anti Human ASK1 (MBS223333)
anti-ASK1 antibody Testing Data #1 image
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 Testing Data #2 of anti-ASK1 antibody    Immunocytochemical staining of A431 epithelial carcinoma cell line with Rabbit anti Human ASK1 (MBS223333)
anti-ASK1 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for ASK1. It may not necessarily be applicable to this product.
 NCBI GI #    5174547
 NCBI GeneID    4217
 NCBI Accession #    NP_005914.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005923.3 [Other Products]
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 UniProt Primary Accession #    Q99683 [Other Products]
 UniProt Secondary Accession #    Q5THN3; Q99461; A6NIA0; B4DGB2 [Other Products]
 UniProt Related Accession #    Q99683 [Other Products]
 Molecular Weight    69,839 Da
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 NCBI Official Full Name    mitogen-activated protein kinase kinase kinase 5
 NCBI Official Synonym Full Names    mitogen-activated protein kinase kinase kinase 5
 NCBI Official Symbol    MAP3K5 [Similar Products]
 NCBI Official Synonym Symbols   
ASK1; MEKK5; MAPKKK5
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase kinase kinase 5; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1
 UniProt Protein Name    Mitogen-activated protein kinase kinase kinase 5
 UniProt Synonym Protein Names   
Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
 Protein Family    DASH complex
 UniProt Gene Name    MAP3K5 [Similar Products]
 UniProt Synonym Gene Names    ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5 [Similar Products]
 UniProt Entry Name    M3K5_HUMAN
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 NCBI Summary for ASK1    Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ASK1    ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.25; Protein kinase, STE; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytosol

Molecular Function: protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; magnesium ion binding; caspase activator activity; protein phosphatase binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of JNK activity; viral reproduction; activation of MAPKK activity; positive regulation of apoptosis; MAPKKK cascade; positive regulation of JNK cascade; positive regulation of caspase activity; protein amino acid phosphorylation; activation of JNK activity; innate immune response; JNK cascade; induction of apoptosis by oxidative stress
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 Research Articles on ASK1    1. TNF-signaling dependence of ASK1-mediated apoptosis in melanoma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ASK1 antibodyPathways associated with anti-ASK1 antibody
 Reference Product  PubMed Publications
 TRAF2 antibody  >23 publications with ASK1 and TRAF2
 DAXX antibody  >17 publications with ASK1 and DAXX
 MAP2K6 antibody  >11 publications with ASK1 and MAP2K6
 TXN antibody  >10 publications with ASK1 and TXN
 MAP2K3 antibody  >9 publications with ASK1 and MAP2K3
 TRAF6 antibody  >9 publications with ASK1 and TRAF6
 MAP2K7 antibody  >5 publications with ASK1 and MAP2K7
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway antibodies  HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
Diseases associated with anti-ASK1 antibodyOrgans/Tissues associated with anti-ASK1 antibody
 Disease Name  Pubmed Publications
 Death Antibodies  >136 publications with ASK1 and Death
 Necrosis Antibodies  >71 publications with ASK1 and Necrosis
 Nervous System Diseases Antibodies  >35 publications with ASK1 and Nervous System Diseases
 Inflammation Antibodies  >27 publications with ASK1 and Inflammation
 Brain Diseases Antibodies  >25 publications with ASK1 and Brain Diseases
 Disease Models, Animal Antibodies  >23 publications with ASK1 and Disease Models, Animal
 Heart Diseases Antibodies  >13 publications with ASK1 and Heart Diseases
 Liver Diseases Antibodies  >11 publications with ASK1 and Liver Diseases
 Hypertrophy Antibodies  >10 publications with ASK1 and Hypertrophy
 Lung Diseases Antibodies  >10 publications with ASK1 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with ASK1 and Muscle
 Brain Antibodies  >27 publications with ASK1 and Brain
 Heart Antibodies  >25 publications with ASK1 and Heart
 Liver Antibodies  >18 publications with ASK1 and Liver
 Kidney Antibodies  >15 publications with ASK1 and Kidney
 Lung Antibodies  >12 publications with ASK1 and Lung
 Bone Antibodies  >11 publications with ASK1 and Bone
 Nerve Antibodies  >11 publications with ASK1 and Nerve
 Embryonic Tissue Antibodies  >8 publications with ASK1 and Embryonic Tissue
 Prostate Antibodies  >7 publications with ASK1 and Prostate
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