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| Product Name
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ATF6, Polyclonal Antibody
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| Also Known As
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Rabbit Polyclonal to Human ATF6
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| Product Synonym Names
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Anti-ATF6 Antibody (N-Terminus) IHC-plus; ATF6; ATF6-alpha; ATF6A; Human ATF6
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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605537
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| 3D Structure
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ModBase 3D Structure for P18850
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| Clonality
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Polyclonal
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse
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| Specificity
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17 amino acid peptide from near the amino terminus of human ATF6
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| Purity/Purification
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Immunoaffinity Purified
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| Form/Format
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PBS, 0.02% sodium azide.
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| Concentration
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1 mg/ml (lot specific)
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| Target Species
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Human
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| Immunogen Description
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Synthetic peptide from human ATF6.
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| Immunogen Type
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Synthetic peptide
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| Immunogen
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ATF6 antibody was raised against synthetic peptide from human ATF6.
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| Antigen Modification
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N-Terminus
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| Preparation and Storage
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Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
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| Other Notes
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Small volumes of anti-ATF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Activating Transcription Factor 6 (ATF6) is a type II transmembrane glycoprotein located on the ER membrane. In response to ER stress ATF6 is cleaved and the N-terminus including the leucine zipper (which faces the cytosol) moves to the nucleus. Once in the nucleus ATF6 binds to the ER stress response elements in ER stress genes. Site-1 and site-2 protease enzymes that process sterol regulatory binding proteins in response to cholesterol derivation cleave ATF6.
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| Applications Tested/Suitable for anti-ATF6 antibody
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Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
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| Application Notes for anti-ATF6 antibody
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ELISA, ICC, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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| Immunohistochemistry (IHC) of anti-ATF6 antibody
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Anti-ATF6 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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| Immunocytochemistry (ICC) of anti-ATF6 antibody
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Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 ug/ml.
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| Western Blot (WB) of anti-ATF6 antibody
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Western blot of ATF6 in MDA-MB-361 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/ml.
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TOP
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| NCBI/Uniprot data below describe general gene information for ATF6. It may not necessarily be applicable to this product. |
| NCBI GI #
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56786157
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| NCBI GeneID
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22926
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| NCBI Accession #
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NP_031374.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_007348.3
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[Other Products]
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| UniProt Primary Accession #
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P18850
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[Other Products]
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| UniProt Secondary Accession #
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O15139; Q5VW62; Q6IPB5; Q9UEC9
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[Other Products]
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| UniProt Related Accession #
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P18850
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[Other Products]
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| Molecular Weight
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74,585 Da
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TOP
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| NCBI Official Full Name
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cyclic AMP-dependent transcription factor ATF-6 alpha
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| NCBI Official Synonym Full Names
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activating transcription factor 6
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| NCBI Official Symbol
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ATF6
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[Similar Products]
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| NCBI Official Synonym Symbols
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ATF6A
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[Similar Products]
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| NCBI Protein Information
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cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
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| UniProt Protein Name
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Cyclic AMP-dependent transcription factor ATF-6 alpha
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| UniProt Synonym Protein Names
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Activating transcription factor 6 alpha; ATF6-alpha
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| Protein Family
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Cyclic AMP-dependent transcription factor
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| UniProt Gene Name
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ATF6
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[Similar Products]
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| UniProt Synonym Gene Names
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cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
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[Similar Products]
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| UniProt Entry Name
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ATF6A_HUMAN
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| NCBI Summary for ATF6
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This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
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| UniProt Comments for ATF6
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ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.
Protein type: Membrane protein, integral; Transcription factor
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus
Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; protein folding; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction
Disease: Achromatopsia 7
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TOP
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| Research Articles on ATF6
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1. Data shows that mutation of putative p38 MAPK phosphorylation sites in ATF6 suppresses its transcriptional induction of DAPK1.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-ATF6 antibody | Pathways associated with anti-ATF6 antibody |
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| Diseases associated with anti-ATF6 antibody | Organs/Tissues associated with anti-ATF6 antibody |
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Antibody 
