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anti-ACTA1 antibody :: Rabbit Actin-Skeletal Muscle Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS300923
Immunohistochemistry (IHC)
Unit / Price
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 Product Name   

Actin-Skeletal Muscle (ACTA1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human Actin-Skeletal Muscle Polyclonal Antibody

 Product Gene Name   

anti-ACTA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    102610
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Bovine, Chicken, Frog, Mouse, Pig, Rabbit, Rat
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 Form/Format    0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Synthetic peptide derived from N-terminus of human alpha skeletal muscle isoform of actin.
 Control Tissue    Skeletal Muscle
 Cellular Localization    Cytoplasm
 Entrez Gene Code    58
 Pathway    Muscle Markers
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ACTA1 antibody

   This antibody reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas).
 Applications Tested/Suitable for anti-ACTA1 antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-ACTA1 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: No special pretreatment necessary. Antigen Retrieval: No special pretreatment necessary. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization Visualization: Detect the antibody as instructed by the instructions provided with the visualization
Immunohistochemistry Dilution: 1:200
IHC Positive Control: Skeletal Muscle
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 Immunohistochemistry (IHC) of anti-ACTA1 antibody    Human skeletal muscle stained with anti-actin antibody
anti-ACTA1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.
 NCBI GI #    4501881
 NCBI GeneID    58
 NCBI Accession #    NP_001091.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001100.3 [Other Products]
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 UniProt Secondary Accession #    P02568; P99020; Q5T8M9 [Other Products]
 UniProt Related Accession #    P68133 [Other Products]
 Molecular Weight    42 kDa [Similar Products]
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 NCBI Official Full Name    actin, alpha skeletal muscle
 NCBI Official Synonym Full Names    actin, alpha 1, skeletal muscle
 NCBI Official Symbol    ACTA1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    actin, alpha skeletal muscle; nemaline myopathy type 3
 UniProt Protein Name    Actin, alpha skeletal muscle
 UniProt Synonym Protein Names   
 UniProt Gene Name    ACTA1 [Similar Products]
 UniProt Synonym Gene Names    ACTA [Similar Products]
 UniProt Entry Name    ACTS_HUMAN
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 NCBI Summary for ACTA1    The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACTA1    ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
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 Research Articles on ACTA1    1. The predominant pathway mediated by Australian bat lyssavirus G envelope for internalization into HEK293T cells is clathrin-and actin-dependent also requiring Rab5.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ACTA1 antibodyPathways associated with anti-ACTA1 antibody
 Reference Product  PubMed Publications
 TPM3 antibody  >30 publications with ACTA1 and TPM3
 ACTC1 antibody  >2 publications with ACTA1 and ACTC1
 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Hypothetical Network For Drug Addiction Pathway antibodies  Hypothetical Network For Drug Addiction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 RhoA Signaling Pathway antibodies  RhoA Signaling Pathway Diagram
 Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies  Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with anti-ACTA1 antibodyOrgans/Tissues associated with anti-ACTA1 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >91 publications with ACTA1 and Nervous System Diseases
 Heart Diseases Antibodies  >19 publications with ACTA1 and Heart Diseases
 Hypertrophy Antibodies  >17 publications with ACTA1 and Hypertrophy
 Cardiovascular Diseases Antibodies  >16 publications with ACTA1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >14 publications with ACTA1 and Disease Models, Animal
 Fibrosis Antibodies  >6 publications with ACTA1 and Fibrosis
 Brain Diseases Antibodies  >4 publications with ACTA1 and Brain Diseases
 Hepatomegaly Antibodies  >2 publications with ACTA1 and Hepatomegaly
 Liver Diseases Antibodies  >2 publications with ACTA1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >126 publications with ACTA1 and Muscle
 Heart Antibodies  >29 publications with ACTA1 and Heart
 Brain Antibodies  >13 publications with ACTA1 and Brain
 Embryonic Tissue Antibodies  >7 publications with ACTA1 and Embryonic Tissue
 Nerve Antibodies  >5 publications with ACTA1 and Nerve
 Bone Antibodies  >5 publications with ACTA1 and Bone
 Lung Antibodies  >3 publications with ACTA1 and Lung
 Adipose Tissue Antibodies  >2 publications with ACTA1 and Adipose Tissue
 Eye Antibodies  >2 publications with ACTA1 and Eye
 Vascular Antibodies  >2 publications with ACTA1 and Vascular
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