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anti-Akt-S473 antibody :: Rabbit Akt-S473 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9132872
Western Blot (WB)
Unit / Price
0.05 mL  /  $305 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt-S473, Polyclonal Antibody

 Also Known As   

Phospho-Akt-S473 Polyclonal Antibody

 Product Synonym Names    AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
 Product Gene Name   

anti-Akt-S473 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M63167 mRNA
 3D Structure    ModBase 3D Structure for P31749
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity Purification
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 Immunogen    A phospho specific peptide corresponding to residues surrounding S473 of human Akt
 Storage Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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 Preparation and Storage    Store at -20 degree C. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-Akt-S473 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-Akt-S473 antibody    Primary antibody
 Applications Tested/Suitable for anti-Akt-S473 antibody   

Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

 Application Notes for anti-Akt-S473 antibody    WB: 1:500 - 1:1000
IF: 1:50- 1:200
IP: 1:50- 1:200
FC: 1:50- 1:200
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 Western Blot (WB) of anti-Akt-S473 antibody    Western blot analysis of extracts of NIH3T3 cells, usingPhospho-Akt-S473 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
anti-Akt-S473 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Akt-S473. It may not necessarily be applicable to this product.
 NCBI GI #    60391226
 NCBI GeneID    207
 NCBI Accession #    P31749.2 [Other Products]
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 UniProt Primary Accession #    P31749 [Other Products]
 UniProt Secondary Accession #    Q9BWB6; B2RAM5; B7Z5R1 [Other Products]
 UniProt Related Accession #    P31749 [Other Products]
 Molecular Weight    56 kDa
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 NCBI Official Full Name    RAC-alpha serine/threonine-protein kinase
 NCBI Official Synonym Full Names    AKT serine/threonine kinase 1
 NCBI Official Symbol    AKT1 [Similar Products]
 NCBI Official Synonym Symbols   
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
 UniProt Gene Name    AKT1 [Similar Products]
 UniProt Synonym Gene Names    PKB; RAC; PKB; PKB alpha [Similar Products]
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 NCBI Summary for Akt-S473    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for Akt-S473    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: AGC group; AKT family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: cytoplasm; cytosol; microtubule cytoskeleton; nucleoplasm; nucleus; plasma membrane; vesicle

Molecular Function: ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: activated T cell apoptosis; cell differentiation; cell proliferation; cellular response to insulin stimulus; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; I-kappaB kinase/NF-kappaB cascade; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of caspase activity; negative regulation of fatty acid beta-oxidation; negative regulation of protein kinase activity; negative regulation of proteolysis; nitric oxide biosynthetic process; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; phosphorylation; platelet activation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell growth; positive regulation of cellular protein metabolic process; positive regulation of endodeoxyribonuclease activity; positive regulation of endothelial cell proliferation; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fat cell differentiation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of lipid biosynthetic process; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein import into nucleus, translocation; protein modification process; regulation of cell migration; regulation of glycogen biosynthetic process; regulation of mRNA stability; regulation of nitric-oxide synthase activity; regulation of phosphoinositide 3-kinase cascade; response to heat; response to oxidative stress; signal transduction; T cell costimulation

Disease: Breast Cancer; Colorectal Cancer; Cowden Syndrome 6; Ovarian Cancer; Proteus Syndrome; Schizophrenia
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 Research Articles on Akt-S473    1. High AKT1 expression is Associated with Endometrial Cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Akt-S473 antibodyPathways associated with anti-Akt-S473 antibody
 Reference Product  PubMed Publications
 PTEN antibody  >511 publications with Akt-S473 and PTEN
 MTOR antibody  >432 publications with Akt-S473 and MTOR
 FOXO1 antibody  >130 publications with Akt-S473 and FOXO1
 MDM2 antibody  >51 publications with Akt-S473 and MDM2
 TSC2 antibody  >39 publications with Akt-S473 and TSC2
 PIK3CB antibody  >19 publications with Akt-S473 and PIK3CB
 TERT antibody  >14 publications with Akt-S473 and TERT
 GSK3B antibody  >9 publications with Akt-S473 and GSK3B
 UBC antibody  >6 publications with Akt-S473 and UBC
 HSP90AA1 antibody  >3 publications with Akt-S473 and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
Diseases associated with anti-Akt-S473 antibodyOrgans/Tissues associated with anti-Akt-S473 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >403 publications with Akt-S473 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >350 publications with Akt-S473 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >301 publications with Akt-S473 and Nervous System Diseases
 Necrosis Antibodies  >296 publications with Akt-S473 and Necrosis
 Lung Neoplasms Antibodies  >234 publications with Akt-S473 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >195 publications with Akt-S473 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >194 publications with Akt-S473 and Prostatic Neoplasms
 Inflammation Antibodies  >179 publications with Akt-S473 and Inflammation
 Heart Diseases Antibodies  >150 publications with Akt-S473 and Heart Diseases
 Colorectal Neoplasms Antibodies  >136 publications with Akt-S473 and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >786 publications with Akt-S473 and Blood
 Muscle Antibodies  >681 publications with Akt-S473 and Muscle
 Vascular Antibodies  >674 publications with Akt-S473 and Vascular
 Brain Antibodies  >480 publications with Akt-S473 and Brain
 Lung Antibodies  >361 publications with Akt-S473 and Lung
 Heart Antibodies  >302 publications with Akt-S473 and Heart
 Liver Antibodies  >294 publications with Akt-S473 and Liver
 Nerve Antibodies  >244 publications with Akt-S473 and Nerve
 Prostate Antibodies  >241 publications with Akt-S473 and Prostate
 Kidney Antibodies  >171 publications with Akt-S473 and Kidney
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