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anti-Akt1 antibody :: Rabbit anti-Human, Mouse Akt Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS631310 anti-Akt1 antibody
Unit / Price
10 Blots  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt (Akt1), Polyclonal Antibody

 Also Known As   

Akt, phosphorylated (Thr308) (Rac PKa, PKBa)

 Product Synonym Names    Anti -Akt, phosphorylated (Thr308) (Rac PKa, PKBa)
 Product Gene Name   

anti-Akt1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 3R; NT_033777.2 (11924938..11930239). Location: 89B3-89B3
 3D Structure    ModBase 3D Structure for Q8INB9
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    Recognizes human Akt1 when phosphorylated at Thr308. Species Crossreactivity
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
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 Immunogen    Synthetic peptide corresponding to the region of human Akt1 that contains threonine 308.
 Positive Control    3T3-L1 pre-adipocytes +/- PDGF treatment; HEK293 cells coexperessing RasV12 and RacL61
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-Akt1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Akt1 antibody

   Akt, also known as Protein Kinase B (PKB) or RAC-a, is a 60kD serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3b, caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt.
 Product Categories/Family for anti-Akt1 antibody    Antibodies; Abs to Protein Kinases
 Applications Tested/Suitable for anti-Akt1 antibody   

ELISA (EL/EIA), Western Blot (WB)

 Application Notes for anti-Akt1 antibody    Suitable for use in ELISA and Western Blot
Dilution: Western Blot: 1:1000
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NCBI/Uniprot data below describe general gene information for Akt1. It may not necessarily be applicable to this product.
 NCBI GI #    24647360
 NCBI GeneID    41957
 NCBI Accession #    NP_732115.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_169707.1 [Other Products]
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 UniProt Primary Accession #    Q8INB9 [Other Products]
 UniProt Secondary Accession #    Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7 [Other Products]
 UniProt Related Accession #    Q24469 [Other Products]
 Molecular Weight    68,485 Da [Similar Products]
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 NCBI Official Full Name    Akt1, isoform B
 NCBI Official Symbol    Akt1 [Similar Products]
 NCBI Official Synonym Symbols   
akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
[Similar Products]
 NCBI Protein Information    CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
 UniProt Protein Name    RAC serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Akt; Protein kinase B
 Protein Family    AKT-interacting protein
 UniProt Gene Name    Akt1 [Similar Products]
 UniProt Synonym Gene Names    DAkt; DRAC-PK; Dakt1; PKB [Similar Products]
 UniProt Entry Name    AKT1_DROME
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 UniProt Comments for Akt1    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: EC 2.7.11.1; Oncoprotein; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: circadian rhythm; oogenesis; positive regulation of cell size; regulation of dendrite development; chitin-based embryonic cuticle biosynthetic process; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of organ growth; positive regulation of cell growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; response to oxidative stress; lipid metabolic process; regulation of cell size; negative regulation of apoptosis
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 Research Articles on Akt1    1. Hippo signaling not only blocks cell division and promotes apoptosis, but also regulates cellular growth by inhibiting the Akt pathway activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Akt1 antibodyDiseases associated with anti-Akt1 antibody
 Products by Pathway  Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 Activation Of PKB Pathway antibodies  Activation Of PKB Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1891 publications with Akt1 and Neoplasms
 Breast Neoplasms Antibodies  >362 publications with Akt1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >317 publications with Akt1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >278 publications with Akt1 and Nervous System Diseases
 Necrosis Antibodies  >276 publications with Akt1 and Necrosis
 Lung Neoplasms Antibodies  >198 publications with Akt1 and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >184 publications with Akt1 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >175 publications with Akt1 and Neoplasms, Experimental
 Inflammation Antibodies  >154 publications with Akt1 and Inflammation
 Heart Diseases Antibodies  >137 publications with Akt1 and Heart Diseases
Organs/Tissues associated with anti-Akt1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >732 publications with Akt1 and Blood
 Embryonic Tissue Antibodies  >124 publications with Akt1 and Embryonic Tissue
 Head Antibodies  >75 publications with Akt1 and Head
 Ovary Antibodies  >58 publications with Akt1 and Ovary
 Fat Body Antibodies  >29 publications with Akt1 and Fat Body
 Whole Body Antibodies  >28 publications with Akt1 and Whole Body
 Uncharacterized Tissue Antibodies  >3 publications with Akt1 and Uncharacterized Tissue
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