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anti-Akt antibody :: Rabbit Akt Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9405780
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Akt, Polyclonal Antibody

 Also Known As   

Akt (Ab-308) Antibody

 Product Synonym Names    RAC-PK-alpha; Protein kinase B
 Product Gene Name   

anti-Akt antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114480
 3D Structure    ModBase 3D Structure for P31749
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    The antibody detects endogenous level of total Akt protein.
 Purity/Purification    Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
 Form/Format    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
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 Immunogen Type    Peptide-KLH
 Immunogen Description    Peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.
 Target Name    Akt
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 Preparation and Storage    Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
 Other Notes    Small volumes of anti-Akt antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Akt antibody

   General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
 Product Categories/Family for anti-Akt antibody    Total protein Ab
 Applications Tested/Suitable for anti-Akt antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-Akt antibody    Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
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 Western Blot (WB) of anti-Akt antibody    Western blot analysis of extracts from HepG2 and Hela cells using Akt(Ab-308) Antibody.
anti-Akt antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-Akt antibody    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Ab-308) Antibody (left) or the same antibody preincubated with blocking peptide(right).
anti-Akt antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for Akt. It may not necessarily be applicable to this product.
 NCBI GI #    62241013
 NCBI GeneID    207
 NCBI Accession #    NP_001014431.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001014431.1 [Other Products]
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 UniProt Primary Accession #    P31749 [Other Products]
 UniProt Secondary Accession #    Q9BWB6; B2RAM5; B7Z5R1 [Other Products]
 UniProt Related Accession #    P31749 [Other Products]
 Molecular Weight    48,347 Da
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 NCBI Official Full Name    RAC-alpha serine/threonine-protein kinase
 NCBI Official Synonym Full Names    v-akt murine thymoma viral oncogene homolog 1
 NCBI Official Symbol    AKT1 [Similar Products]
 NCBI Official Synonym Symbols   
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
 Protein Family    AKT-interacting protein
 UniProt Gene Name    AKT1 [Similar Products]
 UniProt Synonym Gene Names    PKB; RAC; PKB; PKB alpha [Similar Products]
 UniProt Entry Name    AKT1_HUMAN
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 NCBI Summary for Akt    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
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 UniProt Comments for Akt    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: microtubule cytoskeleton; nucleoplasm; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; regulation of cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; positive regulation of fat cell differentiation; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; response to oxidative stress; regulation of nitric-oxide synthase activity; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; nitric oxide biosynthetic process; cell differentiation; inflammatory response; activated T cell apoptosis; aging; epidermal growth factor receptor signaling pathway; negative regulation of proteolysis; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; osteoblast differentiation; G-protein coupled receptor protein signaling pathway; cell proliferation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; phosphorylation; hyaluronan metabolic process

Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
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 Research Articles on Akt    1. BMCC1 negatively regulates phosphorylation pathway of AKT, resulted in apoptosis and the BNIP2 homology region of BMCC1 interacts with BCL2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Akt antibodyPathways associated with anti-Akt antibody
 Reference Product  PubMed Publications
 PTEN antibody  >540 publications with Akt and PTEN
 MTOR antibody  >466 publications with Akt and MTOR
 FOXO1 antibody  >136 publications with Akt and FOXO1
 NOS3 antibody  >128 publications with Akt and NOS3
 FOXO3 antibody  >68 publications with Akt and FOXO3
 MDM2 antibody  >52 publications with Akt and MDM2
 ILK antibody  >37 publications with Akt and ILK
 RICTOR antibody  >24 publications with Akt and RICTOR
 GSK3B antibody  >9 publications with Akt and GSK3B
 HSP90AA1 antibody  >3 publications with Akt and HSP90AA1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
Diseases associated with anti-Akt antibodyOrgans/Tissues associated with anti-Akt antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >422 publications with Akt and Breast Neoplasms
 Nervous System Diseases Antibodies  >321 publications with Akt and Nervous System Diseases
 Necrosis Antibodies  >307 publications with Akt and Necrosis
 Insulin Resistance Antibodies  >253 publications with Akt and Insulin Resistance
 Lung Neoplasms Antibodies  >253 publications with Akt and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >205 publications with Akt and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >202 publications with Akt and Prostatic Neoplasms
 Inflammation Antibodies  >191 publications with Akt and Inflammation
 Heart Diseases Antibodies  >158 publications with Akt and Heart Diseases
 Colorectal Neoplasms Antibodies  >144 publications with Akt and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >815 publications with Akt and Blood
 Muscle Antibodies  >707 publications with Akt and Muscle
 Vascular Antibodies  >698 publications with Akt and Vascular
 Brain Antibodies  >511 publications with Akt and Brain
 Lung Antibodies  >390 publications with Akt and Lung
 Heart Antibodies  >319 publications with Akt and Heart
 Liver Antibodies  >314 publications with Akt and Liver
 Nerve Antibodies  >254 publications with Akt and Nerve
 Prostate Antibodies  >247 publications with Akt and Prostate
 Kidney Antibodies  >177 publications with Akt and Kidney
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