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anti-A2M antibody :: Goat Alpha-2-Macroglobulin / A2M Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS247265
Immunohistochemistry (IHC)
Unit / Price
0.5 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Alpha-2-Macroglobulin / A2M, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat Polyclonal (IgG) to Human Alpha-2-Macroglobulin / A2M

 Product Synonym Names    Anti-Alpha-2-Macroglobulin / A2M Antibody IHC-plus; CPAMD5; A2MD; FWP007; Alpha-2-M; Alpha-2-macroglobulin; S863-7; Human Alpha-2-Macroglobulin; A2M
 Product Gene Name   

anti-A2M antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    103950
 3D Structure    ModBase 3D Structure for P01023
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Goat
 Species Reactivity    Dog, Human, Pig, Rabbit
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 Specificity    Recognizes Macroglobulin, alpha2. Species cross-reactivity: Human, rabbit, porcine and canine.
 Purity/Purification    Ammonium sulfate precipitation
 Form/Format    0.01 M HEPES, pH 7.4, 0.1 M sodium chloride, 50% glycerol.
 Concentration    10 mg/ml (lot specific)
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 Immunogen Species    A2M/Alpha-2 Macroglobulin antibody was raised against Human.
 Antigen Type    Purified protein
 Immunogen    Alpha-2-Macroglobulin / A2M antibody was raised against human Macroglobulin, alpha2 purified from plasma.
 Target    A2M/Alpha-2-Macroglobulin
 Disclaimer    Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
 Recommended Immunohistochemistry Protocol    The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
 Note    During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
 Warning    This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
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 Preparation and Storage    Short term 4C, long term aliquot and store at -20C, avoid freeze thaw cycles. Store undiluted.
 Other Notes    Small volumes of anti-A2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-A2M antibody

   Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits.
 Applications Tested/Suitable for anti-A2M antibody   

Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry (paraffin) and Immunoelectrophoresis.

 Application Notes for anti-A2M antibody    IHC-Paraffin (5 ug/mL)

Optimal dilution to be determined by the researcher.
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 Immunohistochemistry (IHC) of anti-A2M antibody    Anti-Alpha-2-Macroglobulin / A2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
anti-A2M antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for A2M. It may not necessarily be applicable to this product.
 NCBI GI #    66932947
 NCBI GeneID    2
 NCBI Accession #    NP_000005.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000014.4 [Other Products]
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 UniProt Primary Accession #    P01023 [Other Products]
 UniProt Secondary Accession #    Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97 [Other Products]
 UniProt Related Accession #    P01023 [Other Products]
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 NCBI Official Full Name    alpha-2-macroglobulin
 NCBI Official Synonym Full Names    alpha-2-macroglobulin
 NCBI Official Symbol    A2M [Similar Products]
 NCBI Official Synonym Symbols   
A2MD; CPAMD5; FWP007; S863-7
[Similar Products]
 NCBI Protein Information    alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 UniProt Protein Name    Alpha-2-macroglobulin
 UniProt Synonym Protein Names   
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
 Protein Family    Alpha-2-macroglobulin
 UniProt Gene Name    A2M [Similar Products]
 UniProt Synonym Gene Names    CPAMD5; Alpha-2-M [Similar Products]
 UniProt Entry Name    A2MG_HUMAN
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 NCBI Summary for A2M    Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
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 UniProt Comments for A2M    A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; regulation of small GTPase mediated signal transduction; extracellular matrix organization and biogenesis; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
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 Research Articles on A2M    1. genetic variation within the A2M gene influences CSF Ab42 levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-A2M antibodyPathways associated with anti-A2M antibody
 Reference Product  PubMed Publications
 APP antibody  >17 publications with A2M and APP
 LRP1 antibody  >11 publications with A2M and LRP1
 ALB antibody  >6 publications with A2M and ALB
 APOA1 antibody  >4 publications with A2M and APOA1
 PLG antibody  >2 publications with A2M and PLG
 TGFB1 antibody  >2 publications with A2M and TGFB1
 SERPINA1 antibody  >1 publications with A2M and SERPINA1
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 HDL-mediated Lipid Transport Pathway antibodies  HDL-mediated Lipid Transport Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
Diseases associated with anti-A2M antibodyOrgans/Tissues associated with anti-A2M antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >95 publications with A2M and Nervous System Diseases
 Mental Disorders Antibodies  >83 publications with A2M and Mental Disorders
 Alzheimer Disease Antibodies  >80 publications with A2M and Alzheimer Disease
 Inflammation Antibodies  >32 publications with A2M and Inflammation
 Necrosis Antibodies  >14 publications with A2M and Necrosis
 Liver Diseases Antibodies  >14 publications with A2M and Liver Diseases
 Lung Diseases Antibodies  >13 publications with A2M and Lung Diseases
 Disease Models, Animal Antibodies  >11 publications with A2M and Disease Models, Animal
 Fibrosis Antibodies  >11 publications with A2M and Fibrosis
 Carcinoma Antibodies  >8 publications with A2M and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >125 publications with A2M and Blood
 Liver Antibodies  >38 publications with A2M and Liver
 Brain Antibodies  >30 publications with A2M and Brain
 Vascular Antibodies  >15 publications with A2M and Vascular
 Lung Antibodies  >13 publications with A2M and Lung
 Heart Antibodies  >9 publications with A2M and Heart
 Prostate Antibodies  >9 publications with A2M and Prostate
 Uterus Antibodies  >6 publications with A2M and Uterus
 Ovary Antibodies  >6 publications with A2M and Ovary
 Embryonic Tissue Antibodies  >6 publications with A2M and Embryonic Tissue
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