|
| Product Name
|
|
Ankyrin-1 (ANK1), Polyclonal Antibody
|
| Also Known As
|
|
Ankyrin-1 Antibody
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
612641
|
| 3D Structure
|
|
ModBase 3D Structure for P16157
|
|
| Clonality
|
|
Polyclonal
|
| Host
|
|
Rabbit
|
| Species Reactivity
|
|
Human
|
|
|
| Form/Format
|
|
anti-ANK1 antibody purified by peptide affinity column containing 1% BSA and 0.02% NaN3
|
| Concentration
|
|
100ug/200ul (lot specific)
|
|
|
| Source Note
|
|
Rabbit polyclonal ANK1 (1) antibody was raised against a peptide from the N terminal residues of human ANK1 protein.
|
| Reactivity Note
|
|
Predicted: Mouse, Rat; Not yet tested in other species.
|
| Pathway
|
|
Ankyrin-1
|
|
|
| Other Notes
|
|
Small volumes of anti-ANK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
| Product Categories/Family for anti-ANK1 antibody
|
|
Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
|
|
| Applications Tested/Suitable for anti-ANK1 antibody
|
|
Western Blot (WB)
|
| Application Notes for anti-ANK1 antibody
|
|
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1:1,000
Optimal dilutions/concentrations should be determined by the end use.
|
|
|
| Testing Data of anti-ANK1 antibody
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for ANK1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
70780357
|
| NCBI GeneID
|
|
286
|
| NCBI Accession #
|
|
NP_000028.3
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_000037.3
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P16157
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
O43400; Q13768; Q53ER1; Q59FP2; Q8N604; Q99407; A0PJN8; A6NJ23; E5RFL7
|
[Other Products]
|
| UniProt Related Accession #
|
|
P16157
|
[Other Products]
|
| Molecular Weight
|
|
17,854 Da
|
[Similar Products]
|
|
TOP
|
| NCBI Official Full Name
|
|
ankyrin-1 isoform 3
|
| NCBI Official Synonym Full Names
|
|
ankyrin 1, erythrocytic
|
| NCBI Official Symbol
|
|
ANK1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
ANK; SPH1; SPH2
|
[Similar Products]
|
| NCBI Protein Information
|
|
ankyrin-1; ANK-1; ankyrin-R; erythrocyte ankyrin
|
| UniProt Protein Name
|
|
Ankyrin-1
|
| UniProt Synonym Protein Names
|
|
Ankyrin-R; Erythrocyte ankyrin
|
| Protein Family
|
|
Ankyrin
|
| UniProt Gene Name
|
|
ANK1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
ANK; ANK-1
|
[Similar Products]
|
| UniProt Entry Name
|
|
ANK1_HUMAN
|
|
|
| NCBI Summary for ANK1
|
|
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]
|
|
|
| UniProt Comments for ANK1
|
|
ANK1: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. Defects in ANK1 are a cause of spherocytosis type 1 (SPH1); also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive. 21 isoforms of the human protein are produced by alternative promoter.
Protein type: Adaptor/scaffold; Cell development/differentiation; Endoplasmic reticulum; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p11.1
Cellular Component: cortical cytoskeleton; postsynaptic membrane; cytoskeleton; sarcoplasmic reticulum; basolateral plasma membrane; plasma membrane; M band; axolemma; nucleus; Z disc; cytosol; sarcolemma
Molecular Function: protein binding; enzyme binding; spectrin binding; structural constituent of cytoskeleton; cytoskeletal adaptor activity; structural molecule activity; ATPase binding
Biological Process: axon guidance; erythrocyte development; ER to Golgi vesicle-mediated transport; iron ion homeostasis; exocytosis; monovalent inorganic cation transport; cytoskeleton organization and biogenesis; signal transduction; porphyrin biosynthetic process; maintenance of epithelial cell polarity
Disease: Spherocytosis, Type 1
|
|
TOP
|
|
Product References and Citations for anti-ANK1 antibody
|
|
(1) Harder,M.N., Ribel-Madsen,R., Justesen,J.M.,. et al. Type 2 diabetes risk alleles near BCAR1 and in ANK1 associate with decreased beta-cell function whereas risk alleles near ANKRD55 and GRB14 associate with decreased insulin sensitivity in the Danish Inter99 cohort. J. Clin. Endocrinol. Metab. 98 (4), E801-E806 (2013)
|
|
|
| Research Articles on ANK1
|
|
1. ANK1 rs516946 confers impaired insulin release.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with anti-ANK1 antibody | Pathways associated with anti-ANK1 antibody |
|
|
|
| Diseases associated with anti-ANK1 antibody | Organs/Tissues associated with anti-ANK1 antibody |
|
|
|
|
|
|
TOP
|
Antibody 
