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anti-Apaf-1 antibody :: Rabbit anti-Human Apaf-1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS440041 anti-Apaf-1 antibody
Unit / Price
0.2 mg  /  $240 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Apaf-1, Polyclonal Antibody

 Also Known As   

Apaf-1 (A15) Antibody

 Product Gene Name   

anti-Apaf-1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602233
 3D Structure    ModBase 3D Structure for O14727
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Human Apaf-1
 Form/Format    200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
 Concentration    0.200 mg/ml (lot specific)
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 Origin    Apaf-1 (A15) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminus of human Apaf-1.
 Immunogen    Synthetic peptide mapping at the amino terminal domain of human PCNA
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 Preparation and Storage    Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
 Other Notes    Small volumes of anti-Apaf-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Apaf-1 antibody

   Large multi-protein complexes known as the DISC (death inducing signalling complex) and the apoptosome activate the normally inactive caspases through proteolytic processing (1). The formation of these complexes leads to the activation of caspases and the execution of apoptosis (1,2). The apoptosome is a large 700 or 1400 kDa protein complex comprised of Apaf-1 (apoptotic protease activating factor-1), cytochrome c, and dATP (1). Apaf-1 and cytochrome c in the presence of dATP leads to the oligomerization of Apaf-1 and the formation of either the 700 or 1400 kDa complexes (3&4). Once formed the apoptosome activates caspase-9, which in turn activates the effector caspases (1&5). Some researchers have found the 700 kDa apoptosome to be the most effective activator of the effector caspases, when compared to the 1400 kDa apoptosome (3&4). Apaf-1 has also been shown to be a target of regulation by a variety of proteins that include p53, IAPs, heat shock proteins and Smac/Diablo (1&6).
 Applications Tested/Suitable for anti-Apaf-1 antibody   

Western Blot (WB)

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NCBI/Uniprot data below describe general gene information for Apaf-1. It may not necessarily be applicable to this product.
 NCBI GI #    20141188
 NCBI GeneID    317
 NCBI Accession #    O14727.2 [Other Products]
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 UniProt Primary Accession #    O14727 [Other Products]
 UniProt Secondary Accession #    O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8 [Other Products]
 UniProt Related Accession #    O14727 [Other Products]
 Molecular Weight    37,976 Da
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 NCBI Official Full Name    Apoptotic protease-activating factor 1
 NCBI Official Synonym Full Names    apoptotic peptidase activating factor 1
 NCBI Official Symbol    APAF1 [Similar Products]
 NCBI Official Synonym Symbols   
CED4; APAF-1
[Similar Products]
 NCBI Protein Information    apoptotic protease-activating factor 1
 UniProt Protein Name    Apoptotic protease-activating factor 1
 UniProt Gene Name    APAF1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0413; APAF-1 [Similar Products]
 UniProt Entry Name    APAF_HUMAN
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 NCBI Summary for Apaf-1    This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Apaf-1    APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: cytosol; nucleus; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: caspase activation; regulation of apoptosis; nervous system development; neuron apoptosis; apoptosis; positive regulation of apoptosis; neural tube closure; forebrain development; caspase activation via cytochrome c
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Product References and Citations for anti-Apaf-1 antibody

   1. Cain K., Bratton S.B., Cohen G.M. 2002. The Apaf-1 apoptosome: a large caspase-activating complex. Biochimie 84 (2-3):203-214.2. Bratton S.B., Lewis J., Butterworth M., Duckett C.S., Cohen G.M. 2002. XIAP inhibition of caspase-3 preserves its association with the Apaf-1 apoptosome and prevents CD95- and Bax-induced apoptosis. Cell Death Differ 9(9):881-892.3. Bratton S.B., Walker G., Roberts D.L., Cain K., Cohen G.M. 2001. Caspase-3 cleaves Apaf-1 into an ~30kDa fragment that associates with an inappropriately oligomerized and biologically inactive ~1.4 Mda apoptosome complex. Cell Death Differ 8(4):425-433.4. Cain K., Bratton S.B., Langlais C., Walker G., Brown D.G., Sun X.M., Cohen G.M. 2000. Apaf-1 oligomerizes into biologically active approximately 700-kDa and inactive approximately 1.4-Mda apoptosome complex. J Biol Chem 275(9):6067-6070.5. Zou H., Li Y., Liu X., Wang X. 1999. An Apaf-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem 274(17):11549-11556.6. Robles A.I., Bemmels N.A., Foraker A.B., Harris C.C. 2001. Apaf-1 is a transcriptional target of p53 in DNA damage-induced apoptosis. Cancer Res 61(18):6660-6664.
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 Research Articles on Apaf-1    1. IL-24 has a role in sensitizing melanoma cells to erlotinib through modulation of the Apaf-1 and Akt signaling pathways
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Apaf-1 antibodyDiseases associated with anti-Apaf-1 antibody
 Products by Pathway  Pathway Diagram
 Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway antibodies  Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >90 publications with Apaf-1 and Necrosis
 Nervous System Diseases Antibodies  >79 publications with Apaf-1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >28 publications with Apaf-1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >26 publications with Apaf-1 and Breast Neoplasms
 Inflammation Antibodies  >22 publications with Apaf-1 and Inflammation
 Liver Diseases Antibodies  >21 publications with Apaf-1 and Liver Diseases
 Colonic Neoplasms Antibodies  >17 publications with Apaf-1 and Colonic Neoplasms
 Neoplasms, Experimental Antibodies  >17 publications with Apaf-1 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >17 publications with Apaf-1 and Liver Neoplasms
 Nerve Degeneration Antibodies  >16 publications with Apaf-1 and Nerve Degeneration
Organs/Tissues associated with anti-Apaf-1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >104 publications with Apaf-1 and Brain
 Blood Antibodies  >78 publications with Apaf-1 and Blood
 Liver Antibodies  >49 publications with Apaf-1 and Liver
 Muscle Antibodies  >47 publications with Apaf-1 and Muscle
 Heart Antibodies  >36 publications with Apaf-1 and Heart
 Embryonic Tissue Antibodies  >29 publications with Apaf-1 and Embryonic Tissue
 Kidney Antibodies  >29 publications with Apaf-1 and Kidney
 Eye Antibodies  >26 publications with Apaf-1 and Eye
 Skin Antibodies  >26 publications with Apaf-1 and Skin
 Testis Antibodies  >14 publications with Apaf-1 and Testis
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