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anti-ASNS antibody :: Rabbit anti-Human Asparagine Synthetase Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2027582 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $175 +1 FREE 8GB USB
0.1 mg  /  $225 +1 FREE 8GB USB
0.2 mg  /  $340 +1 FREE 8GB USB
1 mg  /  $800 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Asparagine Synthetase (ASNS), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Asparagine Synthetase (ASNS)

 Product Gene Name   

anti-ASNS antibody

[Similar Products]
 Matching Pairs    Antibody: Asparagine Synthetase (MBS2027582)
Antigen: Asparagine Synthetase (MBS2030240)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
APC-CY7 Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072787)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
PE Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072788)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
APC-CY7 Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072789)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
Cy3 Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072790)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
FITC Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072791)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
HRP Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2072792)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
 Matching Pairs    Unconjugated Antibody: Asparagine Synthetase (MBS2027582)
Biotin Conjugated Antibody: Asparagine Synthetase (ASNS) (MBS2095488)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    M27396 mRNA
 3D Structure    ModBase 3D Structure for P08243
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against ASNS. It has beenselected for its ability to recognize ASNS in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Liquid
 Concentration    200ug/ml (lot specific)
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 Fragment    ASNS (His213~Tyr536)
 Organism Species    Homo sapiens (Human)
 Conjugate    No Conjugate
 Immunogen    Recombinant ASNS (His213~Tyr536) expressed in E Coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2072789
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-ASNS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-ASNS antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-ASNS antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

 Application Notes for anti-ASNS antibody    Western blotting: 1-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.
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 Western Blot (WB) Knockout Verification of anti-ASNS antibody    Knockout Verification: Lane 1: Wild-type K562 cell lysate; Lane 2: ASNS knockout K562 cell lysate; Predicted MW: 64,62,54kd Observed MW: 62kd Primary Ab: 3ug/ml Rabbit Anti-Human ASNS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-ASNS antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-ASNS antibody    Western Blot; Sample: Lane1: Human Jurkat Cells; Lane2: Human K-562 Cells.
anti-ASNS antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-ASNS antibody    Western Blot; Sample: Recombinant ASNS, Human.
anti-ASNS antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-ASNS antibody    DAB staining on IHC-P. Samples: Human Tissue)
anti-ASNS antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for ASNS. It may not necessarily be applicable to this product.
 NCBI GI #    296010848
 NCBI GeneID    440
 NCBI Accession #    NP_001171546.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001178075.1 [Other Products]
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 UniProt Primary Accession #    P08243 [Other Products]
 UniProt Secondary Accession #    P08184; Q15666; Q549T9; Q96HD0; A4D1I8; B4DXZ1; B7ZAA9; D6W5R3; E9PCI3; E9PCX6 [Other Products]
 UniProt Related Accession #    P08243 [Other Products]
 Molecular Weight    54,818 Da
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 NCBI Official Full Name    asparagine synthetase
 NCBI Official Synonym Full Names    asparagine synthetase (glutamine-hydrolyzing)
 NCBI Official Symbol    ASNS [Similar Products]
 NCBI Official Synonym Symbols   
TS11; ASNSD
[Similar Products]
 NCBI Protein Information    asparagine synthetase [glutamine-hydrolyzing]
 UniProt Protein Name    Asparagine synthetase [glutamine-hydrolyzing]
 UniProt Synonym Protein Names   
Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase
 Protein Family    Asparagine synthetase
 UniProt Gene Name    ASNS [Similar Products]
 UniProt Synonym Gene Names    TS11 [Similar Products]
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 NCBI Summary for ASNS    The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
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 UniProt Comments for ASNS    ASNS: asparagine synthetase, an ubiquitous enzyme in mammalian organs, is involved in the first step of the synthesis of L-asparagine from L-glutamine and L-aspartate. Highly regulated in response to cell stress. Induced by dietary protein deficiency, imbalanced amino acid intake via the amino acid response, or ER stress via the EIF2AK3-eIF2-ATF4 branch of the unfolded protein response (UPR). Expression induced by nutrient deprivation or hypoxia in solid tumors. Essential for brain development but apparently not for that of other organs. Deficiency impairs brain development resulting in microcephaly and progressive cerebral atrophy, as well as deficiencies in learning and memory. The mechanism through which ASNS induces neuronal damage may be via damage induced by excessive excitability induced by elevated levels of aspartate/glutamate, via asparagine deficiency, or both. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Cell development/differentiation; EC 6.3.5.4; Energy Metabolism - nitrogen; Ligase

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: cytosol

Molecular Function: asparagine synthase (glutamine-hydrolyzing) activity; ATP binding; cofactor binding; protein binding; protein homodimerization activity

Biological Process: amino acid biosynthetic process; asparagine biosynthetic process; cellular response to glucose starvation; cellular response to hormone stimulus; glutamine metabolic process; liver development; negative regulation of apoptosis; positive regulation of mitotic cell cycle; response to amino acid; response to follicle-stimulating hormone stimulus; response to light stimulus; response to mechanical stimulus; response to methotrexate; response to toxin

Disease: Asparagine Synthetase Deficiency
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 Research Articles on ASNS    1. Transient knockdown of ASNS inhibited cell proliferation and colony formation in AGS and MKN-45 cells. Stable knockdown of ASNS conferred sensitivity to cisplatin in these cells. Depletion of ASNS and cisplatin treatment exerted synergistic effects on tumor growth in AGS xenografts. Moreover, ASNS was found to be up-regulated in human gastric cancer tissues compared with matched normal colon tissues.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ASNS antibodyPathways associated with anti-ASNS antibody
 Reference Product  PubMed Publications
 ATF4 antibody  >20 publications with ASNS and ATF4
 ASS1 antibody  >2 publications with ASNS and ASS1
 GOT1 antibody  >1 publications with ASNS and GOT1
 Products by Pathway  Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
Diseases associated with anti-ASNS antibodyOrgans/Tissues associated with anti-ASNS antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >7 publications with ASNS and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >4 publications with ASNS and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >4 publications with ASNS and Breast Neoplasms
 Liver Neoplasms Antibodies  >3 publications with ASNS and Liver Neoplasms
 Liver Diseases Antibodies  >3 publications with ASNS and Liver Diseases
 Fibrosis Antibodies  >2 publications with ASNS and Fibrosis
 Hypertension Antibodies  >2 publications with ASNS and Hypertension
 Seizures Antibodies  >2 publications with ASNS and Seizures
 Carcinoma, Hepatocellular Antibodies  >2 publications with ASNS and Carcinoma, Hepatocellular
 Heart Diseases Antibodies  >2 publications with ASNS and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with ASNS and Blood
 Liver Antibodies  >7 publications with ASNS and Liver
 Lung Antibodies  >6 publications with ASNS and Lung
 Bone Antibodies  >6 publications with ASNS and Bone
 Brain Antibodies  >6 publications with ASNS and Brain
 Bone Marrow Antibodies  >4 publications with ASNS and Bone Marrow
 Vascular Antibodies  >4 publications with ASNS and Vascular
 Nerve Antibodies  >3 publications with ASNS and Nerve
 Thyroid Antibodies  >3 publications with ASNS and Thyroid
 Ovary Antibodies  >3 publications with ASNS and Ovary
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