View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List B-Amyloid [1-28] IgG fraction Polyclonal Antibody Catalog # MBS352029 Unit / Price 0.1 mL / $720 +1 FREE 8GB USB 5x0.1 mL / $3,125 +3 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name B-Amyloid [1-28] IgG fraction, Polyclonal Antibody Full Product Name B-Amyloid [1-28] IgG fraction Product Synonym Names beta-Amyloid [1-28]; IgG Fraction of Rabbit Anti-beta-Amyloid (1-28) Serum Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Specificity Polypeptide ::: % Cross Reactivitybeta-Amyloid (1-28) ::: 100 Form/Format Antiserum Specificity Other Notes Small volumes of anti-B-Amyloid [1-28] IgG fraction antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-B-Amyloid [1-28] IgG fraction antibody This IgG fraction was prepared by precipitation. dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with ? -amyloid(1-28) covalently attached onto a carrier protein. The antiserum has been shown to be specific for the amino terminal of ? -amyloid and has been characterized by western blotting. ELISA. and cell staining techniques. This IgG fraction is suitable for immunocytochemical. immunoprecipitation and western immunoblotting of ? -amyloid. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum. or with additional buffer for more dilute antibody. This vial contains 2.2 mg of purified IgG Product Categories/Family for anti-B-Amyloid [1-28] IgG fraction antibody Histochemical Grade Antibodies Applications Tested/Suitable for anti-B-Amyloid [1-28] IgG fraction antibody Immunocytochemical Staining, Western Immunoblot, Western Blot Application Notes for anti-B-Amyloid [1-28] IgG fraction antibody Immunocytochemistry: This IgG fraction has been found to stain beta-amyloid using ABC techniques at a dilution of 1:5000.Western Blot: Western immunoblots have been successful at a dilution of 1:500.Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and elecrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in 1:5 diluted evaporated goat milk. using TBS/Tween-20 buffer as diluent.2. Wash x 2 with TBS/Tween-20.3. Apply the rabbit polyclonal antibody after dilution to at least 1:800(Note:higher dilutions may be needed). Use 1% normal goat serum in 1:5 diluted evaporated goat milk as buffer for the primary antibody. Dilute the condensed goat milk with TBS/Tween-20. Let the primary antibody bind for 2-4 hours.4. Wash x 3 with TBS/Tween-20.5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500(dilution may vary depending upon supplier) in 1% normal goat serum in 1:5 diluted evaporated goat milk(useTBS/Tween-20 to dilute the goat milk). Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash x 3 and then soak the membrane overnight in a fairly large volume of TBS/Tween-20.7. Develop color using the DAB reaction or the enhanced DAB reaction. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP