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anti-BRAF antibody :: Rabbit B-Raf (N-terminus) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS472011
Testing Data
Unit / Price
0.1 mL  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

B-Raf (N-terminus) (BRAF), Polyclonal Antibody

 Also Known As   

B-Raf (N-terminus)

 Product Synonym Names    p94; v-Raf murine sarcoma viral oncogene homolog B1
 Product Gene Name   

anti-BRAF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 7; NC_000007.13 (140433812..140624564, complement). Location: 7q34
 OMIM    114500
 3D Structure    ModBase 3D Structure for P15056
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse
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 Specificity    This antibody was affinity purified using B-Raf (Nterminus) peptide (without carrier). The antibody detects 105 and 95 kDa* bands corresponding to the molecular mass of B-Raf. The doublet observed in Jurkat cells may represent multipe isoforms of the B-Raf, since all bands are blocked specifically with B-Raf (N-terminus) peptide.
 Purity/Purification    Affinity Purified
 Form/Format    Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
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 Immunogen    A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human B-Raf. This sequence has high homology with similar regions in rat and mouse B-Raf, and has low homology to other Raf family members.
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 Preparation and Storage    Store at -20 degree C. Do not aliquot. Stable for 1 year.
 Other Notes    Small volumes of anti-BRAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-BRAF antibody

   The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, BRaf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf (S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.
 Applications Tested/Suitable for anti-BRAF antibody   

ELISA, Western Blot

 Application Notes for anti-BRAF antibody    ELISA: 1:2000
Western Blot: 1:1000
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 Testing Data of anti-BRAF antibody    anti-BRAF antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for BRAF. It may not necessarily be applicable to this product.
 NCBI GI #    33188459
 NCBI GeneID    673
 NCBI Accession #    NP_004324.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004333.4 [Other Products]
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 UniProt Primary Accession #    P15056 [Other Products]
 UniProt Secondary Accession #    Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66 [Other Products]
 UniProt Related Accession #    P15056; Q75MQ8 [Other Products]
 Molecular Weight    84306 Da [Similar Products]
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 NCBI Official Full Name    serine/threonine-protein kinase B-raf
 NCBI Official Synonym Full Names    v-raf murine sarcoma viral oncogene homolog B1
 NCBI Official Symbol    BRAF [Similar Products]
 NCBI Official Synonym Symbols   
NS7; BRAF1; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase B-raf; p94; OTTHUMP00000212184; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94)
 UniProt Protein Name    Serine/threonine-protein kinase B-raf
 UniProt Synonym Protein Names   
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    BRAF [Similar Products]
 UniProt Synonym Gene Names    BRAF1; RAFB1 [Similar Products]
 UniProt Entry Name    BRAF_HUMAN
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 NCBI Summary for BRAF    This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]
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 UniProt Comments for BRAF    Function: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Binds 2 zinc ions per subunit

Subunit structure: Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1

Subcellular location: Nucleus

By similarity. Cytoplasm. Cell membrane

By similarity. Note: Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes

Tissue specificity: Brain and testis.

Involvement in disease: Note=Defects in BRAF are found in a wide range of cancers. Ref.10Defects in BRAF may be a cause of colorectal cancer (CRC) [

MIM:114500]. Ref.10Defects in BRAF are involved in lung cancer (LNCR) [

MIM:211980]. Ref.10 Ref.23Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [

MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. Ref.10 Ref.26Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [

MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. Ref.10Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [

MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits. Ref.10 Ref.33Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [

MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness. Ref.10 Ref.33Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation. Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD (Ras-binding) domain.

Sequence caution: The sequence AAD43193.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAQ43111.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAQ43112.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAQ43113.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAQ43114.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAQ43115.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAQ43116.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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Product References and Citations for anti-BRAF antibody

   • Mason, C.S. et al. (1999) EMBOJ 18(8):2137.
• Wilhelm, S.M. et al. (2004) Cancer Res 64:7099.
• Karbowniczek, M. et al. (2006) J Biol Chem 281(35):25447.
• Brummer, T. et al. (2006) Oncogene 25(47):6262.
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 Research Articles on BRAF    1. Ablation of B-Raf had no significant effect on development of K-Ras oncogene-driven non-small cell lung carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BRAF antibodyPathways associated with anti-BRAF antibody
 Reference Product  PubMed Publications
 HRAS antibody  >84 publications with BRAF and HRAS
 MAP2K1 antibody  >52 publications with BRAF and MAP2K1
 MAPK1 antibody  >46 publications with BRAF and MAPK1
 MAP2K2 antibody  >39 publications with BRAF and MAP2K2
 RAF1 antibody  >27 publications with BRAF and RAF1
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
Diseases associated with anti-BRAF antibodyOrgans/Tissues associated with anti-BRAF antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1828 publications with BRAF and Neoplasms
 Melanoma Antibodies  >615 publications with BRAF and Melanoma
 Colorectal Neoplasms Antibodies  >503 publications with BRAF and Colorectal Neoplasms
 Intestinal Diseases Antibodies  >488 publications with BRAF and Intestinal Diseases
 Head and Neck Neoplasms Antibodies  >470 publications with BRAF and Head and Neck Neoplasms
 Thyroid Neoplasms Antibodies  >462 publications with BRAF and Thyroid Neoplasms
 Adenocarcinoma Antibodies  >323 publications with BRAF and Adenocarcinoma
 Skin Diseases Antibodies  >310 publications with BRAF and Skin Diseases
 Skin Neoplasms Antibodies  >266 publications with BRAF and Skin Neoplasms
 Neoplasm Metastasis Antibodies  >153 publications with BRAF and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >521 publications with BRAF and Thyroid
 Skin Antibodies  >329 publications with BRAF and Skin
 Blood Antibodies  >138 publications with BRAF and Blood
 Lymph Node Antibodies  >113 publications with BRAF and Lymph Node
 Lung Antibodies  >106 publications with BRAF and Lung
 Brain Antibodies  >77 publications with BRAF and Brain
 Heart Antibodies  >61 publications with BRAF and Heart
 Kidney Antibodies  >57 publications with BRAF and Kidney
 Prostate Antibodies  >34 publications with BRAF and Prostate
 Ovary Antibodies  >23 publications with BRAF and Ovary
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