anti-BCAP31 antibody :: Rabbit anti-Human B-cell receptor-associated protein 31 Polyclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Immunogen
Recombinant human B-cell receptor-associated protein 31 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18579 / sc-18580 / sc-48766
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-BCAP31 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
anti-BCAP31 antibody
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in the caspase 8-mediated apoptosis. Microdeletions in this gene are associated with the contiguous ABCD1/DXS1375E deletion syndrome. Two pseudogenes have been identified on chromosome 16. Alternatively spliced transcript variants encoding distinct isoforms have been described although the biological validity of some of the variants has not been determined.
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Applications Tested/Suitable for
anti-BCAP31 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blot (WB) of
anti-BCAP31 antibody
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat liver tissue, Rat brain tissue, Mouse liver tissue, Mouse brain tissue
All lanes: BCAP31 antibody at 4.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28,35 KDa
Observed band size: 28 KDa
Immunohistochemistry (IHC) of
anti-BCAP31 antibody
IHC image of MBS7001073 diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (IHC) of
anti-BCAP31 antibody
IHC image of MBS7001073 diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence (IF) of
anti-BCAP31 antibody
Immunofluorescent analysis of Hela cells using MBS7001073 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunoprecipitation (IP) of
anti-BCAP31 antibody
Immunoprecipitating BCAP31 in mouse liver whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS7001073 in mouse liver whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7001073 (6ug)+ mouse liver whole cell lysate (500ug)
Lane 3: Mouse liver whole cell lysate (10ug)
NCBI/Uniprot data below describe general gene information for
BCAP31. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001132913.1
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NCBI GenBank Nucleotide #
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UniProt Primary Accession #
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UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,752 Da
NCBI Official Full Name
B-cell receptor-associated protein 31 isoform b
NCBI Official Synonym Full Names
B-cell receptor-associated protein 31
NCBI Official Synonym Symbols
CDM; DDCH; BAP31; 6C6-AG; DXS1357E [Similar Products]
NCBI Protein Information
B-cell receptor-associated protein 31
UniProt Protein Name
B-cell receptor-associated protein 31
UniProt Synonym Protein Names
6C6-AG tumor-associated antigen; Protein CDM; p28
UniProt Synonym Gene Names
UniProt Entry Name
BAP31_HUMAN
NCBI Summary for BCAP31
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]
UniProt Comments for BCAP31
Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. May be involved in CASP8-mediated apoptosis.
Research Articles on BCAP31
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice. It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-BCAP31 antibody
Pathways associated with anti-BCAP31 antibody
Diseases associated with anti-BCAP31 antibody
Organs/Tissues associated with anti-BCAP31 antibody
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