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anti-ARID1B antibody :: Rabbit BAF250B Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8241303
Unit / Price
0.03 mL  /  $155 +1 FREE 8GB USB
0.1 mL  /  $220 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BAF250B (ARID1B), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-BAF250B Antibody

 Product Synonym Names    BAF250B; DAN15; KIAA1235; OSA2; AT-rich interactive domain-containing protein 1B; ARID domain-containing protein 1B; BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R
 Product Gene Name   

anti-ARID1B antibody

[Similar Products]
 Antibody/Peptide Pairs    BAF250B peptide (MBS8217012) is used for blocking the activity of BAF250B antibody (MBS8241303)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    614556
 3D Structure    ModBase 3D Structure for Q8NFD5
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Porcine
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 Specificity    Recognizes endogenous levels of BAF250B protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAF250B. The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4C. Upon delivery aliquot and store at -20C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-ARID1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ARID1B antibody

   Rabbit polyclonal antibody to BAF250B
 Applications Tested/Suitable for anti-ARID1B antibody   

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

 Application Notes for anti-ARID1B antibody    WB (1/500 - 1/1000)
IHC (1/100 - 1/200)
IF/IC (1/100 - 1/500)
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 Western Blot (WB) of anti-ARID1B antibody    Western blot analysis of BAF250B expression in HeLa (A), HepG2 (B) whole cell lysates.
anti-ARID1B antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-ARID1B antibody    Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-ARID1B antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-ARID1B antibody    Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
anti-ARID1B antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for ARID1B. It may not necessarily be applicable to this product.
 NCBI GI #    297139703
 NCBI GeneID    57492
 NCBI Accession #    NP_059989.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_017519.2 [Other Products]
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 UniProt Primary Accession #    Q8NFD5 [Other Products]
 UniProt Secondary Accession #    Q5JRD1; Q5VYC4; Q8IZY8; Q8TEV0; Q8TF02; Q99491; Q9ULI5 [Other Products]
 UniProt Related Accession #    Q8NFD5 [Other Products]
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 NCBI Official Full Name    AT-rich interactive domain-containing protein 1B isoform 1
 NCBI Official Synonym Full Names    AT rich interactive domain 1B (SWI1-like)
 NCBI Official Symbol    ARID1B [Similar Products]
 NCBI Official Synonym Symbols   
OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
[Similar Products]
 NCBI Protein Information    AT-rich interactive domain-containing protein 1B
 UniProt Protein Name    AT-rich interactive domain-containing protein 1B
 UniProt Synonym Protein Names   
BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R
 Protein Family    AT-rich interactive domain-containing protein
 UniProt Gene Name    ARID1B [Similar Products]
 UniProt Synonym Gene Names    BAF250B; DAN15; KIAA1235; OSA2; ARID domain-containing protein 1B; BAF250B; hOsa2 [Similar Products]
 UniProt Entry Name    ARI1B_HUMAN
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 NCBI Summary for ARID1B    This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2012]
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 UniProt Comments for ARID1B    ARID1B: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Binds DNA non-specifically. Defects in ARID1B are the cause of mental retardation autosomal dominant type 12 (MRD12). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD12 patients present with moderate to severe psychomotor retardation, and most show evidence of muscular hypotonia. In many patients, expressive speech is more severely affected than receptive function. Additional common findings include short stature, abnormal head shape and low-set, posteriorly rotated, and abnormally shaped ears, downslanting palpebral fissures, a bulbous nasal tip, a thin upper lip, minor teeth anomalies, and brachydactyly or single palmar creases. Autistic features are uncommon. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; SWI/SNF complex; cytoplasm

Molecular Function: protein binding; DNA binding; transcription coactivator activity

Biological Process: nervous system development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription

Disease: Mental Retardation, Autosomal Dominant 12
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 Research Articles on ARID1B    1. BAF complex gene ARID1B is mutated in Coffin-Siris syndrome patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ARID1B antibodyPathways associated with anti-ARID1B antibody
 Reference Product  PubMed Publications
 ARID1A antibody  >31 publications with ARID1B and ARID1A
 SMARCA4 antibody  >15 publications with ARID1B and SMARCA4
 SMARCA2 antibody  >12 publications with ARID1B and SMARCA2
 SMARCB1 antibody  >11 publications with ARID1B and SMARCB1
 SMARCE1 antibody  >9 publications with ARID1B and SMARCE1
 ACTL6A antibody  >1 publications with ARID1B and ACTL6A
 SMARCD1 antibody  >1 publications with ARID1B and SMARCD1
 SMARCC2 antibody  >1 publications with ARID1B and SMARCC2
 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 RMTs Methylate Histone Arginines Pathway antibodies  RMTs Methylate Histone Arginines Pathway Diagram
Diseases associated with anti-ARID1B antibodyOrgans/Tissues associated with anti-ARID1B antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >22 publications with ARID1B and Nervous System Diseases
 Neurobehavioral Manifestations Antibodies  >18 publications with ARID1B and Neurobehavioral Manifestations
 Coffin-Siris syndrome Antibodies  >17 publications with ARID1B and Coffin-Siris syndrome
 Carcinoma Antibodies  >6 publications with ARID1B and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with ARID1B and Adenocarcinoma
 Breast Neoplasms Antibodies  >2 publications with ARID1B and Breast Neoplasms
 Liver Neoplasms Antibodies  >2 publications with ARID1B and Liver Neoplasms
 Hepatomegaly Antibodies  >1 publications with ARID1B and Hepatomegaly
 Memory Disorders Antibodies  >1 publications with ARID1B and Memory Disorders
 Kidney Diseases Antibodies  >1 publications with ARID1B and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with ARID1B and Brain
 Blood Antibodies  >4 publications with ARID1B and Blood
 Bone Antibodies  >2 publications with ARID1B and Bone
 Embryonic Tissue Antibodies  >2 publications with ARID1B and Embryonic Tissue
 Thyroid Antibodies  >2 publications with ARID1B and Thyroid
 Liver Antibodies  >2 publications with ARID1B and Liver
 Bone Marrow Antibodies  >1 publications with ARID1B and Bone Marrow
 Heart Antibodies  >1 publications with ARID1B and Heart
 Mouth Antibodies  >1 publications with ARID1B and Mouth
 Lung Antibodies  >1 publications with ARID1B and Lung
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