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anti-BBC3 antibody :: Goat BBC3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS422523
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BBC3, Polyclonal Antibody

 Also Known As   

Goat anti-BBC3 Antibody

 Product Synonym Names    BBC3; BCL2 binding component 3; JFY1; PUMA; Bcl-2 binding component 3; BCL2 binding component 3 antibody; Bcl-2 binding component 3 antibody; JFY1 antibody; PUMA antibody; BBC3 antibody
 Product Gene Name   

anti-BBC3 antibody

[Similar Products]
 Antibody/Peptide Pairs    BBC3 peptide (MBS427491) is used for blocking the activity of BBC3 antibody (MBS422523)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    DGGRPQRTVR
 OMIM    605854
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-DGGRPQRTVR, from the internal region of the protein sequence according to NP_001120712.1.
 Epitope    Internal region
 Note    This antibody is expected to recognize isoform 1 (NP_001120712.1) only.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-BBC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-BBC3 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-BBC3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 24kDa band observed in Human peripheral blood mononucleocytes lysates (calculated MW of 26.5kDa according to NP_001120712.1). Recommended concentration: 0.1-0.3ug/ml.
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 Western Blot (WB) of anti-BBC3 antibody    (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-BBC3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for BBC3. It may not necessarily be applicable to this product.
 NCBI GI #    187829730
 NCBI GeneID    27113
 NCBI Accession #    NP_001120712.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001127240.2 [Other Products]
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 UniProt Related Accession #    Q9BXH1; Q96PG8 [Other Products]
 Molecular Weight    20,532 Da
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 NCBI Official Full Name    bcl-2-binding component 3 isoform 1
 NCBI Official Synonym Full Names    BCL2 binding component 3
 NCBI Official Symbol    BBC3 [Similar Products]
 NCBI Official Synonym Symbols   
JFY1; PUMA; JFY-1
[Similar Products]
 NCBI Protein Information    bcl-2-binding component 3
 UniProt Protein Name    Bcl-2-binding component 3
 UniProt Synonym Protein Names   
JFY-1; p53 up-regulated modulator of apoptosis
 Protein Family    Bcl-2-binding component
 UniProt Gene Name    BBC3 [Similar Products]
 UniProt Synonym Gene Names    PUMA [Similar Products]
 UniProt Entry Name    BBC3B_HUMAN
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 NCBI Summary for BBC3    This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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 UniProt Comments for BBC3    BBC3B: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity. Induced by DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. {ECO:0000269|PubMed:11463392}. Isoform 3 does not interact with BCL2. 4 isoforms of the human protein are produced by alternative splicing

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cytosol; endoplasmic reticulum; lysosome; mitochondrial outer membrane; mitochondrion

Molecular Function: ATPase binding; glycoprotein binding; protein binding

Biological Process: apoptosis; caspase activation; determination of adult life span; negative regulation of growth; positive regulation of neuron apoptosis; positive regulation of protein homooligomerization; protein insertion into mitochondrial membrane during induction of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; regulation of apoptosis; release of cytochrome c from mitochondria; release of sequestered calcium ion into cytosol; response to DNA damage stimulus
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 Research Articles on BBC3    1. puma-mediated apoptosis is not induced in mucosal melanomas by cisplatin
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-BBC3 antibodyDiseases associated with anti-BBC3 antibody
 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis - Multiple Species Pathway antibodies  Apoptosis - Multiple Species Pathway Diagram
 Apoptosis - Multiple Species Pathway antibodies  Apoptosis - Multiple Species Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >90 publications with BBC3 and Carcinoma
 Adenocarcinoma Antibodies  >40 publications with BBC3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >36 publications with BBC3 and Breast Neoplasms
 Lung Neoplasms Antibodies  >28 publications with BBC3 and Lung Neoplasms
 Colonic Neoplasms Antibodies  >26 publications with BBC3 and Colonic Neoplasms
 Necrosis Antibodies  >25 publications with BBC3 and Necrosis
 Liver Diseases Antibodies  >22 publications with BBC3 and Liver Diseases
 Lung Diseases Antibodies  >22 publications with BBC3 and Lung Diseases
 Nervous System Diseases Antibodies  >18 publications with BBC3 and Nervous System Diseases
 Inflammation Antibodies  >15 publications with BBC3 and Inflammation
Organs/Tissues associated with anti-BBC3 antibody
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >35 publications with BBC3 and Lung
 Brain Antibodies  >21 publications with BBC3 and Brain
 Kidney Antibodies  >15 publications with BBC3 and Kidney
 Bone Antibodies  >14 publications with BBC3 and Bone
 Skin Antibodies  >13 publications with BBC3 and Skin
 Prostate Antibodies  >13 publications with BBC3 and Prostate
 Stomach Antibodies  >12 publications with BBC3 and Stomach
 Muscle Antibodies  >9 publications with BBC3 and Muscle
 Eye Antibodies  >7 publications with BBC3 and Eye
 Intestine Antibodies  >7 publications with BBC3 and Intestine
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