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anti-BHMT antibody :: Goat BHMT Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421546
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BHMT, Polyclonal Antibody

 Also Known As   

Goat anti-BHMT Antibody

 Product Synonym Names    BHMT; betaine-homocysteine methyltransferase; BHMT antibody; betaine-homocysteine methyltransferase antibody
 Product Gene Name   

anti-BHMT antibody

[Similar Products]
 Antibody/Peptide Pairs    BHMT peptide (MBS425943) is used for blocking the activity of BHMT antibody (MBS421546)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    EQQLKELFEK QK
 OMIM    602888
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-EQQLKELFEKQK, from the C Terminus of the protein sequence according to NP_001704.1.
 Epitope    C Terminus
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-BHMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-BHMT antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-BHMT antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong cytoplasm staining in PCT cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 45kDa band observed in Human Liver, Mouse Liver and Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45. 0kDa according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.
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 Western Blot (WB) of anti-BHMT antibody    (0. 03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-BHMT antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-BHMT antibody    HEK293 overexpressing BHMT RC203148) and probed (mock transfection in first lane), tested by Origene.
anti-BHMT antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for BHMT. It may not necessarily be applicable to this product.
 NCBI GI #    4502407
 NCBI GeneID    635
 NCBI Accession #    NP_001704.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001713.1 [Other Products]
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 UniProt Secondary Accession #    Q9UNI9 [Other Products]
 UniProt Related Accession #    Q93088 [Other Products]
 Molecular Weight    44,998 Da
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 NCBI Official Full Name    betaine-homocysteine methyltransferase
 NCBI Official Synonym Full Names    betaine--homocysteine S-methyltransferase
 NCBI Official Symbol    BHMT [Similar Products]
 NCBI Official Synonym Symbols   
BHMT1; HEL-S-61p
[Similar Products]
 NCBI Protein Information    betaine--homocysteine S-methyltransferase 1
 UniProt Protein Name    Betaine--homocysteine S-methyltransferase 1
 Protein Family    Betaine--homocysteine S-methyltransferase
 UniProt Gene Name    BHMT [Similar Products]
 UniProt Entry Name    BHMT1_HUMAN
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 NCBI Summary for BHMT    This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BHMT    BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - cysteine and methionine; Methyltransferase; EC 2.1.1.5; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding

Biological Process: betaine catabolic process; betaine metabolic process; choline catabolic process; protein amino acid methylation; regulation of homocysteine metabolic process; S-adenosylmethionine metabolic process; sulfur amino acid metabolic process
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 Research Articles on BHMT    1. The faster evolutionary rate of BHMT2 overall suggests that selective constraints were reduced relative to BHMT.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BHMT antibodyPathways associated with anti-BHMT antibody
 Reference Product  PubMed Publications
 CBS antibody  >49 publications with BHMT and CBS
 MTHFR antibody  >48 publications with BHMT and MTHFR
 MAT1A antibody  >8 publications with BHMT and MAT1A
 AHCY antibody  >6 publications with BHMT and AHCY
 MAT2B antibody  >5 publications with BHMT and MAT2B
 MAT2A antibody  >5 publications with BHMT and MAT2A
 DMGDH antibody  >3 publications with BHMT and DMGDH
 CTH antibody  >2 publications with BHMT and CTH
 AHCYL2 antibody  >1 publications with BHMT and AHCYL2
 AHCYL1 antibody  >1 publications with BHMT and AHCYL1
 Products by Pathway  Pathway Diagram
 Choline Catabolism Pathway antibodies  Choline Catabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 L-methionine Salvage From L-homocysteine Pathway antibodies  L-methionine Salvage From L-homocysteine Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
Diseases associated with anti-BHMT antibodyOrgans/Tissues associated with anti-BHMT antibody
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >37 publications with BHMT and Liver Diseases
 Cardiovascular Diseases Antibodies  >26 publications with BHMT and Cardiovascular Diseases
 Fatty Liver Antibodies  >16 publications with BHMT and Fatty Liver
 Heart Diseases Antibodies  >13 publications with BHMT and Heart Diseases
 Disease Models, Animal Antibodies  >13 publications with BHMT and Disease Models, Animal
 Liver Neoplasms Antibodies  >12 publications with BHMT and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >12 publications with BHMT and Carcinoma, Hepatocellular
 Drug-Induced Liver Injury Antibodies  >11 publications with BHMT and Drug-Induced Liver Injury
 Weight Gain Antibodies  >10 publications with BHMT and Weight Gain
 Kidney Diseases Antibodies  >8 publications with BHMT and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >138 publications with BHMT and Liver
 Kidney Antibodies  >31 publications with BHMT and Kidney
 Brain Antibodies  >13 publications with BHMT and Brain
 Intestine Antibodies  >4 publications with BHMT and Intestine
 Eye Antibodies  >3 publications with BHMT and Eye
 Embryonic Tissue Antibodies  >2 publications with BHMT and Embryonic Tissue
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