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anti-BRAF antibody :: Rabbit anti-Human, Mouse BRAF Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9408755
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BRAF, Polyclonal Antibody

★Popular Item★
 Also Known As   

BRAF Antibody

 Product Synonym Names    V-raf murine sarcoma viral oncogene homolog B1, NS7, BRAF1, RAFB1, B-RAF1
 Product Gene Name   

anti-BRAF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114500
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    The antibody detects endogenous level of total BRAF protein.
 Form/Format    Supplied at 2.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
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 Immunogen Type    Peptide
 Immunogen Description    Synthetic peptide corresponding to a region derived from 591-607 amino acids of Human v-raf murine sarcoma viral oncogene homolog B1
 Target Name    BRAF
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 Preparation and Storage    Store at -20 degree C/1 year
 Other Notes    Small volumes of anti-BRAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BRAF antibody

   This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
 Product Categories/Family for anti-BRAF antibody    Total protein Ab
 Applications Tested/Suitable for anti-BRAF antibody   

ELISA (EIA, Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-BRAF antibody    ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
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 Testing Data of anti-BRAF antibody    Gel: 8%SDS-PAGE
Lane1: Hela cell lysate
Lane2: HT-29 cell lysateLysates: 50 ug per lane
Primary antibody: 1/1400 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 5 minutes
anti-BRAF antibody Testing Data image
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 Immunohistochemistry (IHC) of anti-BRAF antibody    The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31224 (BRAF Antibody) at dilution 1/60, on the right is treated with the synthetic peptide.
anti-BRAF antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for BRAF. It may not necessarily be applicable to this product.
 NCBI GI #    33188459
 NCBI GeneID    673
 NCBI Accession #    NP_004324 [Other Products]
 NCBI GenBank Nucleotide #    NM_004333.4 [Other Products]
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 UniProt Secondary Accession #    Q13878; Q3MIN6; Q9UDP8; Q9Y6T3; A4D1T4; B6HY61; B6HY62; B6HY63; B6HY64; B6HY65; B6HY66 [Other Products]
 UniProt Related Accession #    P15056 [Other Products]
 Molecular Weight    84kD
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 NCBI Official Full Name    serine/threonine-protein kinase B-raf
 NCBI Official Synonym Full Names    B-Raf proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    BRAF [Similar Products]
 NCBI Official Synonym Symbols   
NS7; BRAF1; RAFB1; B-RAF1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase B-raf
 UniProt Protein Name    Serine/threonine-protein kinase B-raf
 UniProt Synonym Protein Names   
Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    BRAF [Similar Products]
 UniProt Synonym Gene Names    BRAF1; RAFB1 [Similar Products]
 UniProt Entry Name    BRAF_HUMAN
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 NCBI Summary for BRAF    This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for BRAF    BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Oncoprotein; TKL group; RAF family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus

Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; positive T cell selection; fibroblast growth factor receptor signaling pathway; response to cAMP; activation of MAPKK activity; CD4-positive, alpha beta T cell differentiation; nerve growth factor receptor signaling pathway; protein heterooligomerization; somatic stem cell maintenance; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis

Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3
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 Research Articles on BRAF    1. we identify that analysis of plasma and urinary cfDNA provides a reliable method to detect the BRAF(V600E) mutation and monitor response to therapy in these disorders.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BRAF antibodyPathways associated with anti-BRAF antibody
 Reference Product  PubMed Publications
 NRAS antibody  >842 publications with BRAF and NRAS
 HRAS antibody  >196 publications with BRAF and HRAS
 MAP2K1 antibody  >135 publications with BRAF and MAP2K1
 MAP2K2 antibody  >73 publications with BRAF and MAP2K2
 RAF1 antibody  >69 publications with BRAF and RAF1
 MAPK3 antibody  >66 publications with BRAF and MAPK3
 KSR1 antibody  >4 publications with BRAF and KSR1
 RAP1A antibody  >2 publications with BRAF and RAP1A
 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
Diseases associated with anti-BRAF antibodyOrgans/Tissues associated with anti-BRAF antibody
 Disease Name  Pubmed Publications
 Melanoma Antibodies  >2484 publications with BRAF and Melanoma
 Neuroendocrine Tumors Antibodies  >1744 publications with BRAF and Neuroendocrine Tumors
 Colorectal Neoplasms Antibodies  >1345 publications with BRAF and Colorectal Neoplasms
 Thyroid Neoplasms Antibodies  >1043 publications with BRAF and Thyroid Neoplasms
 Microsatellite Instability Antibodies  >617 publications with BRAF and Microsatellite Instability
 Lung Neoplasms Antibodies  >381 publications with BRAF and Lung Neoplasms
 Lung Diseases Antibodies  >339 publications with BRAF and Lung Diseases
 Nervous System Diseases Antibodies  >324 publications with BRAF and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >297 publications with BRAF and Neoplasms, Experimental
 Glioma Antibodies  >220 publications with BRAF and Glioma
 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >1288 publications with BRAF and Thyroid
 Skin Antibodies  >1154 publications with BRAF and Skin
 Lung Antibodies  >560 publications with BRAF and Lung
 Blood Antibodies  >455 publications with BRAF and Blood
 Lymph Node Antibodies  >443 publications with BRAF and Lymph Node
 Brain Antibodies  >400 publications with BRAF and Brain
 Kidney Antibodies  >93 publications with BRAF and Kidney
 Prostate Antibodies  >65 publications with BRAF and Prostate
 Ovary Antibodies  >42 publications with BRAF and Ovary
 Pancreas Antibodies  >41 publications with BRAF and Pancreas
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