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anti-BRD4 antibody :: Rabbit BRD4 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS126660
Western Blot (WB)
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

BRD4, Polyclonal Antibody

★Popular Item★
 Also Known As   

BRD4

 Product Synonym Names    BRD4
 Product Gene Name   

anti-BRD4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 19; NC_000019.9 (15348301..15443354, complement). Location: 19p13.1
 OMIM    608749
 3D Structure    ModBase 3D Structure for O60885
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, mouse, rat
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 Purity/Purification    Affinity Purification
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 Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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 Preparation and Storage    Store at -20C or -80C. Avoid freeze / thaw cycles.
 Other Notes    Small volumes of anti-BRD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BRD4 antibody

   Bromodomain-containing protein 4 (BRD4) is a member of the bromodomairls and extraterminal (BET) family of proteins, which also includes BRD2, BRD3, and BRDT (1-3). BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues (3). BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4 (4). BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced (5). DRD4 tlssocia::oll with chromatin uuring mitosis is thought to be an important part of the bookmarking mechanism to accelerate re-activation of the silenced genes upon exit from mitosis (2,6). BRD4 has been shown to facilitate transcription by recruiting the positive transcription elongation factor b (pTEFb) complex that phosphorylates Ser2 of the heptapeptide repeat of the C-terminal domain of RNA polymerase II, promoting transcription elongation (3,7,8). In addition, BRD4 has been found to be part of the super elongation complex and the polymerase associated factor complex (PAFc) in MLL-fusion derived leukemia cell lines, demonstrating a role for BRD4 in the regulation of transcription elongation (9). Research studies have shown that BRD4 (and BET family proteins) may be promising theraeupic targets for various Myc-driven cancers, such as Burkitt's lymphoma and certain acute myeloid leukemias (1,10,11). Investigators have found molecular inhibition of BET proteins to be effective in inducing apoptosis in various MLL-fusion driven leukemic cell lines by competing BRD3 and BRD4 from chromatin, leading to reduced expression of BcI-2, Myc, and CDK6 (9). BET inhibition has also been shown to have antitumor activities against nuclear protein in testis (NUT) midline carcinoma cell lines and xenografts in mice where BRD4 is found to be a frequent translocation partner of the NUT protein (12). In addition, BRD4 regulates the expression of some inflammatory genes, and inhibition of BRD4 (and BET family proteins) chromatin binding causes reduced expression of a subset of inflammatory genes in macrophages, leading to protection against endotoxic shock and sepsis (13).
 Applications Tested/Suitable for anti-BRD4 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

 Application Notes for anti-BRD4 antibody    Recommended Dilutions:

WB 1 :500 - 1 :2000
IHC 1:50-1:200
ICC 1:100-1:250
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 Western Blot (WB) of anti-BRD4 antibody    Western blot analysis of extracts of various cell lines, using BRD4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
anti-BRD4 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for BRD4. It may not necessarily be applicable to this product.
 NCBI GI #    7657218
 NCBI GeneID    23476
 NCBI Accession #    NP_055114.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014299.2 [Other Products]
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 UniProt Primary Accession #    O60885 [Other Products]
 UniProt Secondary Accession #    O60433; Q4G0X8; Q86YS8; Q96PD3 [Other Products]
 UniProt Related Accession #    O60885 [Other Products]
 Molecular Weight    152kDa [Similar Products]
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 NCBI Official Full Name    bromodomain-containing protein 4 isoform short
 NCBI Official Synonym Full Names    bromodomain containing 4
 NCBI Official Symbol    BRD4 [Similar Products]
 NCBI Official Synonym Symbols   
CAP; MCAP; HUNK1; HUNKI
[Similar Products]
 NCBI Protein Information    bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
 UniProt Protein Name    Bromodomain-containing protein 4
 UniProt Synonym Protein Names   
Protein HUNK1
 Protein Family    Bromodomain-containing protein
 UniProt Gene Name    BRD4 [Similar Products]
 UniProt Synonym Gene Names    HUNK1 [Similar Products]
 UniProt Entry Name    BRD4_HUMAN
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BRD4 antibodyDiseases associated with anti-BRD4 antibody
 Reference Product  PubMed Publications
 CDK9 antibody  >25 publications with BRD4 and CDK9
 HEXIM1 antibody  >11 publications with BRD4 and HEXIM1
 CCNT1 antibody  >4 publications with BRD4 and CCNT1
 RFC1 antibody  >1 publications with BRD4 and RFC1
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >13 publications with BRD4 and Inflammation
 Breast Neoplasms Antibodies  >8 publications with BRD4 and Breast Neoplasms
 Musculoskeletal Diseases Antibodies  >7 publications with BRD4 and Musculoskeletal Diseases
 Hypertrophy Antibodies  >3 publications with BRD4 and Hypertrophy
 Liver Neoplasms Antibodies  >2 publications with BRD4 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with BRD4 and Liver Diseases
 Adenocarcinoma Antibodies  >2 publications with BRD4 and Adenocarcinoma
 Atrophy Antibodies  >2 publications with BRD4 and Atrophy
 Mammary Neoplasms, Animal Antibodies  >2 publications with BRD4 and Mammary Neoplasms, Animal
 Fibrosis Antibodies  >2 publications with BRD4 and Fibrosis
Organs/Tissues associated with anti-BRD4 antibody
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >23 publications with BRD4 and Testis
 Blood Antibodies  >14 publications with BRD4 and Blood
 Lung Antibodies  >9 publications with BRD4 and Lung
 Lymph Node Antibodies  >4 publications with BRD4 and Lymph Node
 Liver Antibodies  >4 publications with BRD4 and Liver
 Lymph Antibodies  >4 publications with BRD4 and Lymph
 Bone Antibodies  >4 publications with BRD4 and Bone
 Thymus Antibodies  >3 publications with BRD4 and Thymus
 Kidney Antibodies  >3 publications with BRD4 and Kidney
 Muscle Antibodies  >3 publications with BRD4 and Muscle
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