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anti-lacZ antibody :: Rabbit Beta d Galactosidase (E. coli) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS315025 anti-lacZ antibody
Unit / Price
10 mg  /  $500 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Beta d Galactosidase (E. coli) (lacZ), Polyclonal Antibody

 Also Known As   

Rabbit A' Beta d Galactosidase

 Product Synonym Names    Rabbit Antibody to Beta-d-Galactosidase (E. coli)
 Product Gene Name   

anti-lacZ antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    NC_000913.2 (362455..365529, complement)
 3D Structure    ModBase 3D Structure for P00722
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 Clonality    Polyclonal
 Host    Rabbit
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 Specificity    Beta d Galactosidase (E. coli)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase (E. coli). Recognizes Beta-galactosidase from E. coli. Cross react
 Purity/Purification    Delipidation, salt fractionation and ion-exchange chromatography followed by extensive dialysis against the buffer.
 Form/Format    Purified, Lyophilized
Reconstitute with 1ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.
 Concentration    10mg/ml (OD280nm) (prior to lyophilization) (lot specific)
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 Immunogen    Beta-Galactosidase (E. coli)
 Buffer    Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
 Preservative    0.01% (w/v) Sodium azide
 Lyophilized    Yes
 Important Note    Centrifuge before opening to ensure complete recovery of vial contents.
 Warnings    This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
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 Preparation and Storage    Store lyophilized product at 2 to 8 degree C. After reconstitution, store for several weeks at 2 to 8 degree C as an undiluted liquid. Prepare working dilution only prior to immediate use. Long-term, aliquot and store at -20 degree C or below.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-lacZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-lacZ antibody    Polyclonal Antibodies to Enzymes
 Applications Tested/Suitable for anti-lacZ antibody   

EIA/ELISA, IEP, Immunohistochemistry - Formalin/Paraffin

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NCBI/Uniprot data below describe general gene information for lacZ. It may not necessarily be applicable to this product.
 NCBI GI #    16128329
 NCBI GeneID    945006
 NCBI Accession #    NP_414878.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_000913.2 [Other Products]
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 UniProt Primary Accession #    P00722 [Other Products]
 UniProt Secondary Accession #    Q2MC80 [Other Products]
 Molecular Weight    116,483 Da [Similar Products]
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 NCBI Official Full Name    beta-D-galactosidase
 NCBI Official Symbol    lacZ [Similar Products]
 NCBI Official Synonym Symbols   
ECK0341; JW0335
[Similar Products]
 NCBI Protein Information    beta-D-galactosidase
 UniProt Protein Name    Beta-galactosidase
 UniProt Synonym Protein Names   
Lactase
 Protein Family    Beta-galactosidase
 UniProt Gene Name    lacZ [Similar Products]
 UniProt Entry Name    BGAL_ECOLI
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 NCBI Summary for lacZ    Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].
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 UniProt Comments for lacZ    Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687

Cofactor: Binds 2 magnesium ions per monomer. Can also use manganese. Ref.9 Ref.13 Ref.20Binds 1 sodium ion per monomer. Ref.9 Ref.13 Ref.20

Enzyme regulation: Inhibited by phenylethyl thio-beta-D-galactoside (PETG), isopropyl thio-beta-D-galactoside (IPTG), L-ribose, D-galactonolactone, lactose and 2-amino-D-galactose. Ref.24

Subunit structure: Homotetramer. Ref.21

Induction: By allolactose. Ref.8 Ref.24

Sequence similarities: Belongs to the glycosyl hydrolase 2 family.

Biophysicochemical propertiesKinetic parameters:The values for the enzymatic assays using manganese as cofactor are very close.KM=0.04 mM for p-nitrophenyl beta-D-galactoside Ref.9 Ref.14 Ref.15 Ref.17 Ref.18 Ref.24KM=0.12 mM for o-nitrophenyl beta-D-galactosideKM=0.15 mM for 2,3-dinitrophenyl beta-D-galactopyranosideKM=0.41 mM for 2,5-dinitrophenyl beta-D-galactopyranosideKM=11.6 mM for p-nitrophenol-alpha-L-arabinopyranosideKM=16.9 mM for p-nitrophenol-beta-D-fucopyranosideKM=34 µM for p-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=140 µM for o-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=940 µM for allolactose (with magnesium as cofactor and 30 degrees Celsius)KM=1350 µM for lactose (with magnesium as cofactor and 30 degrees Celsius)Vmax=30.9 µmol/min/mg enzyme with lactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=49.7 µmol/min/mg enzyme with allolactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=59.7 µmol/min/mg enzyme with p-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=360 µmol/min/mg enzyme with o-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)
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Product References and Citations for anti-lacZ antibody

   • Davidson, B.L., et al., (2000), "Recombinant adeno-associated virus type 2, 4 and 5 vectors: Transduction of variant cell types and regions in the mammalian central nervous system", PNAS, 97(7): 3428-3432
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 Research Articles on lacZ    1. Arg-599 of b-galactosidase of Escherichia coli is important as an anchor for the open conformations of Phe-601 and the active-site loop.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-lacZ antibody
 Products by Pathway  Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
 Other Glycan Degradation Pathway antibodies  Other Glycan Degradation Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Lactose Degradation III Pathway antibodies  Lactose Degradation III Pathway Diagram
 Lactose Degradation III Pathway antibodies  Lactose Degradation III Pathway Diagram
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