View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-CD117 (kit) antibody :: Rabbit CD117 (kit) Polyclonal Antibody Catalog # MBS462247 Unit / Price 0.1 mg / $315 +1 FREE 8GB USB 5x0.1 mg / $1,245 +1 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD117 (kit), Polyclonal Antibody Popular Item Full Product Name Rabbit anti CD117/c-Kit (CT) Polyclonal antibody Product Synonym Names Rabbit anti--c-Kit (CT); Kit Protein; c-Kit; CD117 antigen; SCFR; PBT Product Gene Name anti-CD117 (kit) antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Species Reactivity Human, Mouse, Rat, Canine Specificity This antibody recognizes CD17/c-Kit protein. It crossreacts to human, mouse and rat. The other species were not tested. Purity/Purification The Rabbit IgG is purified by Epitope Affinity Purification. Form/Format This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer. Antigen Preparation A synthetic peptide derived from N-terminus of Kit protein. This sequence is identical in human, rat, mouse and dog origins. Positive Control Kidney Tissue Cellular Location Cell Membrane Preparation and Storage The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C.The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles. Other Notes Small volumes of anti-CD117 (kit) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-CD117 (kit) antibody The c-Kit proto-oncogene encodes a 145 kDa transmembrane receptor (CD117) with tyrosine kinase activity that is structurally related to platelet-derived growth factor. C-Kit/CD117 antigen is expressed on almost all hematopoietic stem and progenitor cells. c-Kit regulates a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis. Applications Tested/Suitable for anti-CD117 (kit) antibody ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunprecipitation (IP) Application Notes for anti-CD117 (kit) antibody Western Blot: 0.1-1 ug/mlELISA: 0.01-0.1 ug/mlImmunoprecipitation: 2-5 ug/mlImmunohistochemistry: 2-10 ug/mlOptimal dilutions should be determined by researchers for specific applications. Western Blot (WB) of anti-CD117 (kit) antibody Western Blot: The whole cell lysate derived from Jurkat cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti–CD117/c-Kit (CT) antibody (Cat#MBS462247) at 1:500 . An immunoreactive band around ~145 kDa was observed. Immunohistochemistry (IHC) of anti-CD117 (kit) antibody Immunohistochemistry: Human lymphomacarcinoma (FFPE) stained with Rabbit anti-CD117/kit (Cat# MBS462247) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. TOP Molecular Weight 145 kDa TOP Product References and Citations for anti-CD117 (kit) antibody Li J et al. Expression of c-Kit receptor tyrosine kinase and effect on beta-cell development in the human fetal pancreas. Am J Physiol Endocrinol Metab 293:E475-83 (2007). TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP