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anti-CD28 antibody :: Goat CD28 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS422554
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CD28, Polyclonal Antibody

 Also Known As   

Goat anti-CD28 Antibody

 Product Synonym Names    CD28; CD28 molecule; MGC138290; Tp44; CD28 antigen; CD28 antigen (Tp44); T-cell-specific surface glycoprotein; CD28 antibody; CD28 molecule antibody; MGC138290 antibody; Tp44 antibody; CD28 antigen antibody; CD28 antigen (Tp44) antibody; T-cell-specific surface glycoprotein antibody
 Product Gene Name   

anti-CD28 antibody

[Similar Products]
 Antibody/Peptide Pairs    CD28 peptide (MBS427652) is used for blocking the activity of CD28 antibody (MBS422554)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    SQQLQVYSKT GFNCD
 OMIM    186760
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence SQQLQVYSKTGFNCD, from the internal region of the protein sequence according to NP_006130.1.
 Epitope    Internal region
 Note    The immunizing peptide corresponds to a part of the extracellular domain.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CD28 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-CD28 antibody    Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 48kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
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 Western Blot (WB) of anti-CD28 antibody    (0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CD28 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.
 NCBI GI #    5453611
 NCBI GeneID    940
 NCBI Accession #    NP_006130.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_006139.3 [Other Products]
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 UniProt Secondary Accession #    Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1 [Other Products]
 UniProt Related Accession #    P10747 [Other Products]
 Molecular Weight    26,186 Da
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 NCBI Official Full Name    T-cell-specific surface glycoprotein CD28 isoform 1
 NCBI Official Synonym Full Names    CD28 molecule
 NCBI Official Symbol    CD28 [Similar Products]
 NCBI Official Synonym Symbols   
Tp44
[Similar Products]
 NCBI Protein Information    T-cell-specific surface glycoprotein CD28
 UniProt Protein Name    T-cell-specific surface glycoprotein CD28
 UniProt Synonym Protein Names   
TP44; CD_antigen: CD28
 Protein Family    T-cell-specific surface glycoprotein
 UniProt Gene Name    CD28 [Similar Products]
 UniProt Entry Name    CD28_HUMAN
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 NCBI Summary for CD28    The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
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 UniProt Comments for CD28    CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cell surface; cytosol; external side of plasma membrane; immunological synapse; integral to plasma membrane; plasma membrane

Molecular Function: coreceptor activity; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; protein kinase binding; SH3/SH2 adaptor activity

Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; negative thymic T cell selection; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of alpha-beta T cell proliferation; positive regulation of inflammatory response to antigenic stimulus; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of translation; positive regulation of viral genome replication; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulation of regulatory T cell differentiation; regulatory T cell differentiation; T cell costimulation; T cell receptor signaling pathway
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 Research Articles on CD28    1. Data show that CD28 antigen costimulation modulates CD46 antigen surface expression on activated T cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CD28 antibodyPathways associated with anti-CD28 antibody
 Reference Product  PubMed Publications
 CD80 antibody  >1886 publications with CD28 and CD80
 CD86 antibody  >1140 publications with CD28 and CD86
 LCK antibody  >180 publications with CD28 and LCK
 VAV1 antibody  >64 publications with CD28 and VAV1
 ITK antibody  >48 publications with CD28 and ITK
 GRB2 antibody  >31 publications with CD28 and GRB2
 GRAP2 antibody  >7 publications with CD28 and GRAP2
 CD247 antibody  >1 publications with CD28 and CD247
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
Diseases associated with anti-CD28 antibodyOrgans/Tissues associated with anti-CD28 antibody
 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1305 publications with CD28 and Autoimmune Diseases
 Inflammation Antibodies  >895 publications with CD28 and Inflammation
 Necrosis Antibodies  >891 publications with CD28 and Necrosis
 HIV Infections Antibodies  >466 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >386 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >342 publications with CD28 and Lung Diseases
 Cardiovascular Diseases Antibodies  >340 publications with CD28 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >308 publications with CD28 and Vascular Diseases
 Lymphoma Antibodies  >303 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >258 publications with CD28 and Carcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8521 publications with CD28 and Blood
 Thymus Antibodies  >354 publications with CD28 and Thymus
 Brain Antibodies  >137 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >36 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >27 publications with CD28 and Connective Tissue
 Placenta Antibodies  >21 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >9 publications with CD28 and Adipose Tissue
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