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Add To Compare List anti-CD28 antibody :: Rabbit CD28 Polyclonal Antibody Catalog # MBS9609052 Unit / Price 0.1 mL / $270 +1 FREE 8GB USB 0.2 mL / $315 +1 FREE 8GB USB 5x0.2 mL / $1,245 +1 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CD28, Polyclonal Antibody Popular Item Full Product Name Phospho-CD28 (Tyr218) Antibody Product Synonym Names CD 28; CD28; CD28 antigen; CD28 molecule; CD28_HUMAN; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; TP44 Product Gene Name anti-CD28 antibody[Similar Products] Antibody/Peptide Pairs CD28 (Tyr218) peptide (MBS9627764) is used for blocking the activity of CD28 (Tyr218) antibody (MBS9609052) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP 3D Structure ModBase 3D Structure for P10747 Clonality Polyclonal Isotype IgG Host Rabbit Species Reactivity Human, Mouse, Rat Specificity Phospho-CD28 (Tyr218) antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218 Purity/Purification From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns. Form/Format LiquidPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Concentration 1mg/ml (lot specific) Immunogen A synthesized peptide derived from human Phospho-CD28 (Tyr218) Subcellular Location Membrane. Tissue Specificity Expressed in T-cells and plasma cells, but not in less mature B-cells. Conjugation Unconjugated Epitope Phospho Tyr218 Preparation and Storage Store at -20 degree C. Stable for 12 months from date of receipt. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Supply Chain Verification Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire. Other Notes Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-CD28 antibody Function: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).Subunit Structure: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2. Isoform 3 interacts with CD40LG (PubMed:15067037).Post-translational Modifications: CD40LG induces tyrosine phosphorylation of isoform 3. Applications Tested/Suitable for anti-CD28 antibody Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA) Application Notes for anti-CD28 antibody WB: 1:1000-3000IF/ICC: 1:100-1:500 Western Blot (WB) of anti-CD28 antibody Western blot analysis of Phospho-CD28 (Tyr218) using Jurkat whole cell lysates Immunofluorescene (IF) of anti-CD28 antibody MBS9609052 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody. TOP NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product. NCBI GI # 340545508 NCBI GeneID 940 NCBI Accession # NP_001230006.1 [Other Products] NCBI GenBank Nucleotide # NM_001243077.1 [Other Products] UniProt Primary Accession # P10747 [Other Products] UniProt Secondary Accession # Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1[Other Products] UniProt Related Accession # P10747[Other Products] Molecular Weight Observed: 25 kDaPredicted: 26 kDa TOP NCBI Official Full Name T-cell-specific surface glycoprotein CD28 isoform 2 NCBI Official Synonym Full Names CD28 molecule NCBI Official Symbol CD28 [Similar Products] NCBI Official Synonym Symbols Tp44 [Similar Products] NCBI Protein Information T-cell-specific surface glycoprotein CD28 UniProt Protein Name T-cell-specific surface glycoprotein CD28 UniProt Synonym Protein Names TP44; CD_antigen: CD28 Protein Family T-cell-specific surface glycoprotein UniProt Gene Name CD28 [Similar Products] TOP NCBI Summary for CD28 The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] TOP UniProt Comments for CD28 Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Research Articles on CD28 1. CD28 may be a tumor suppressor gene and rs3116496 polymorphism of CD28 gene showed positively correlation with the increased risk of breast cancer. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with anti-CD28 antibody Reference Product PubMed Publications CD4 antibody >3808 publications with CD28 and CD4 CD80 antibody >1747 publications with CD28 and CD80 CD86 antibody >1035 publications with CD28 and CD86 PTPRC antibody >381 publications with CD28 and PTPRC LCK antibody >159 publications with CD28 and LCK ITK antibody >42 publications with CD28 and ITK GRB2 antibody >27 publications with CD28 and GRB2 GRAP2 antibody >5 publications with CD28 and GRAP2 IL12A antibody >1 publications with CD28 and IL12A DUSP14 antibody >1 publications with CD28 and DUSP14 Pathways associated with anti-CD28 antibody Products by Pathway Pathway Diagram Adaptive Immune System Pathway antibodies Adaptive Immune System Pathway Diagram Allograft Rejection Pathway antibodies Allograft Rejection Pathway Diagram Allograft Rejection Pathway antibodies Allograft Rejection Pathway Diagram Autoimmune Thyroid Disease Pathway antibodies Autoimmune Thyroid Disease Pathway Diagram Autoimmune Thyroid Disease Pathway antibodies Autoimmune Thyroid Disease Pathway Diagram CD28 Co-stimulation Pathway antibodies CD28 Co-stimulation Pathway Diagram CD28 Dependent PI3K/Akt Signaling Pathway antibodies CD28 Dependent PI3K/Akt Signaling Pathway Diagram CD28 Dependent Vav1 Pathway antibodies CD28 Dependent Vav1 Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Diseases associated with anti-CD28 antibody Disease Name Pubmed Publications Immune System Diseases Antibodies >2212 publications with CD28 and Immune System Diseases Autoimmune Diseases Antibodies >1138 publications with CD28 and Autoimmune Diseases Neoplasms Antibodies >1051 publications with CD28 and Neoplasms Necrosis Antibodies >792 publications with CD28 and Necrosis Inflammation Antibodies >713 publications with CD28 and Inflammation HIV Infections Antibodies >424 publications with CD28 and HIV Infections Arthritis, Rheumatoid Antibodies >325 publications with CD28 and Arthritis, Rheumatoid Lung Diseases Antibodies >286 publications with CD28 and Lung Diseases Lymphoma Antibodies >260 publications with CD28 and Lymphoma Carcinoma Antibodies >212 publications with CD28 and Carcinoma Organs/Tissues associated with anti-CD28 antibody Organ/Tissue Name Pubmed Publications Blood Antibodies >7583 publications with CD28 and Blood Thymus Antibodies >323 publications with CD28 and Thymus Brain Antibodies >100 publications with CD28 and Brain Embryonic Tissue Antibodies >56 publications with CD28 and Embryonic Tissue Pharynx Antibodies >34 publications with CD28 and Pharynx Connective Tissue Antibodies >22 publications with CD28 and Connective Tissue Placenta Antibodies >18 publications with CD28 and Placenta Uterus Antibodies >16 publications with CD28 and Uterus Trachea Antibodies >11 publications with CD28 and Trachea Adipose Tissue Antibodies >4 publications with CD28 and Adipose Tissue TOP © 2006-24 MyBioSource.com. 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