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| Product Name
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CD55, Polyclonal Antibody ★Popular Item★
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| Also Known As
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Anti-CD55 Antibody
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| Product Synonym Names
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Complement decay-accelerating factor; CD 55; CD55; CD55 antigen; CD55 Cromer blood group system; CD55 molecule; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay accelerating factor; Complement decay-accelerating factor; Complement decay-accelerating factor, GPI-anchored; CR; CROM; Cromer Blood Group antigen; Cromer blood group system; DAF; Daf-GPI; DAF_HUMAN; Daf1; Dcay accelerating factor for complement (CD55, Cromer blood group system); Decay accelarating factor 1, isoform CRA_a; Decay accelerating factor (GPI-form); Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF; TC; CD55 molecule, decay accelerating factor for complement (Cromer blood group)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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125240
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| 3D Structure
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ModBase 3D Structure for P08174
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| Clonality
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Polyclonal
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| Species Reactivity
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Human, Mouse
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| Specificity
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No cross reactivity with other proteins.
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| Purity/Purification
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Immunogen Affinity Purified
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| Form/Format
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Lyophilized
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| Immunogen
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E Coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
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| Ig Type
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Rabbit IgG
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| Reconstitution
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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| Preparation and Storage
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At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Other Notes
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Small volumes of anti-CD55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Description: Rabbit IgG polyclonal antibody for Complement decay-accelerating factor(CD55) detection. Tested with WB, IHC-P in Human;Mouse.
Background: Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
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| Applications Tested/Suitable for anti-CD55 antibody
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Western Blot (WB), Immunohistochemistry (IHC) Paraffin
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| Application Notes for anti-CD55 antibody
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Western Blot
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Ms
Immunohistochemistry (IHC) Paraffin:
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
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| Western Blot (WB) of anti-CD55 antibody
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Anti- CD55 Picoband antibody, MBS178344, Western blotting
All lanes: Anti CD55 (MBS178344) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
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| Immunohistochemistry (IHC) of anti-CD55 antibody
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Anti- CD55 Picoband antibody, MBS178344, IHC(P)
IHC(P): Human Placenta Tissue
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| NCBI/Uniprot data below describe general gene information for CD55. It may not necessarily be applicable to this product. |
| NCBI GI #
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10835143
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| NCBI GeneID
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1604
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| NCBI Accession #
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NP_000565.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_000574.4
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[Other Products]
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| UniProt Primary Accession #
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P08174
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[Other Products]
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| UniProt Secondary Accession #
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P09679; P78361; Q14UF2; Q14UF3; Q14UF4; Q14UF5; Q14UF6; B1AP14; D3DT83; D3DT84; E7ER69
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[Other Products]
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| UniProt Related Accession #
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P08174
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[Other Products]
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| Molecular Weight
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59,038 Da
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TOP
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| NCBI Official Full Name
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complement decay-accelerating factor isoform 1 preproprotein
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| NCBI Official Synonym Full Names
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CD55 molecule (Cromer blood group)
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| NCBI Official Symbol
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CD55
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[Similar Products]
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| NCBI Official Synonym Symbols
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CR; TC; DAF; CROM
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[Similar Products]
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| NCBI Protein Information
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complement decay-accelerating factor
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| UniProt Protein Name
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Complement decay-accelerating factor
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| UniProt Synonym Protein Names
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CD_antigen: CD55
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| Protein Family
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Complement decay-accelerating factor
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| UniProt Gene Name
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CD55
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[Similar Products]
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| UniProt Synonym Gene Names
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CR; DAF
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[Similar Products]
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| UniProt Entry Name
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DAF_HUMAN
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| NCBI Summary for CD55
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This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
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| UniProt Comments for CD55
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This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
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Product References and Citations for anti-CD55 antibody
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1. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML (April 1987). "Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement". Proc. Natl. Acad. Sci. U.S.A. 84 (7): 2007-11.
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| Research Articles on CD55
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1. present study suggested that the expressions of CD97 antigen and decay accelerating factor DAF were both upregulated in human cervical squamous cell carcinoma
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-CD55 antibody | Pathways associated with anti-CD55 antibody |
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| Diseases associated with anti-CD55 antibody | Organs/Tissues associated with anti-CD55 antibody |
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Antibody 
