Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow CDC25A rightarrow Polyclonal CDC25A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CHRM4 Antibody
 • BRP16 Antibody
 • PLAGL1 Antibody
 • Cytochrome P450 20A1 (CYP20A1) Antibody
 • Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit
 • Death Associated Protein 6 (DAXX) ELISA Kit
 • Aquaporin 4 (AQP-4) ELISA Kit
 • Desmosine ELISA Kit
 • Lactoferrin (LF) ELISA Kit
 • Pan-Cytokeratin (P-CK) ELISA Kit
 • Engulfment and Cell Motility 2 (ELMO2) ELISA Kit
 • Anthrax Protective Antigen IgG (APA IgG) ELISA Kit
 • Cyclophilin D (CYPD) ELISA Kit
 • Skeletal Muscle Actinin-alpha (SM ACTININ-alpha) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-CDC25A antibody :: Rabbit CDC25A Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS126373
Western Blot (WB)
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDC25A, Polyclonal Antibody

 Also Known As   

CDC25A Polyclonal Antibody

 Product Synonym Names    CDC25A2; CDC25A; cdc25A
 Product Gene Name   

anti-CDC25A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    116947
 3D Structure    ModBase 3D Structure for P30304
Section Bar
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
Section Bar
 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
Section Bar
 Species    Human
 Immunogen    Recombinant Protein
 Immunogen    Recombinant protein of human CDC25A
 Calculated Molecular Weight    59kDa
Section Bar
 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-CDC25A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-CDC25A antibody

   The cdc25 protein phosphatase family plays a critical role in activating cyclin-dependent kinases (CDKs) via dephosphorylation of conserved Thr14/Tyr15 inhibitory phosphorylation sites. While cdc25C is primarily responsible for activating CDK1 to overcome the G2/M checkpoint and allow mitotic entry, the primary substrate of cdc25A is CDK2, which, when active, allows progression through the G1/S and intra-S checkpoints (1). Abundance, subcellular localization and activity of cdc25A is tightly controlled by a variety of mechanisms, including phosphorylation, ubiquitination, and inhibitory binding to 14-3-3 proteins. During normal cell cycle progression, elevated c-Myc and E2F transcription factor levels lead to increased cdc25A expression (2). When conditions are favorable for DNA synthesis, cdc25A and CDK2 form an activation loop, wherein each activates the other enzyme (1). DNA damage, on the other hand, leads to multisite phosphorylation at inhibitory sites (Ser123, Ser177, Ser278, Ser292, and Thr506) by Chk1 and Chk2, which result in 14-3-3 binding and ubiquitin-mediated degradation (3,4).
 Product Categories/Family for anti-CDC25A antibody    Polyclonal
 Applications Tested/Suitable for anti-CDC25A antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-CDC25A antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Section Bar
 Western Blot (WB) of anti-CDC25A antibody    Western blot analysis of extracts of various cell lines, using CDC25A antibody.
anti-CDC25A antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CDC25A. It may not necessarily be applicable to this product.
 NCBI GI #    50403734
 NCBI GeneID    993
 NCBI Accession #    P30304.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P30304 [Other Products]
 UniProt Secondary Accession #    Q8IZH5; Q96IL3; Q9H2F2 [Other Products]
 UniProt Related Accession #    P30304 [Other Products]
 Molecular Weight    524
Table BarTOPTable Bar
 NCBI Official Full Name    M-phase inducer phosphatase 1
 NCBI Official Synonym Full Names    cell division cycle 25A
 NCBI Official Symbol    CDC25A [Similar Products]
 NCBI Official Synonym Symbols   
CDC25A2
[Similar Products]
 NCBI Protein Information    M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A
 UniProt Protein Name    M-phase inducer phosphatase 1
 UniProt Synonym Protein Names   
Dual specificity phosphatase Cdc25A
 Protein Family    M-phase inducer phosphatase
 UniProt Gene Name    CDC25A [Similar Products]
 UniProt Entry Name    MPIP1_HUMAN
Section Bar
 NCBI Summary for CDC25A    CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CDC25A    CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; regulation of cell cycle; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
Table BarTOPTable Bar
 Research Articles on CDC25A    1. Findings suggest that inhibition of H19 long non-coding RNA (LncRNAH19) and miR-675 expression can promote migration and invasion of hepatocellular carcinoma (HCC) cells via AKT/GSK-3beta/Cdc25A signaling pathway.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-CDC25A antibodyPathways associated with anti-CDC25A antibody
 Reference Product  PubMed Publications
 CDK2 antibody  >237 publications with CDC25A and CDK2
 CDK1 antibody  >229 publications with CDC25A and CDK1
 CCNB1 antibody  >177 publications with CDC25A and CCNB1
 ATM antibody  >128 publications with CDC25A and ATM
 CHEK2 antibody  >109 publications with CDC25A and CHEK2
 MYC antibody  >106 publications with CDC25A and MYC
 CCNE1 antibody  >7 publications with CDC25A and CCNE1
 CHEK1 antibody  >4 publications with CDC25A and CHEK1
 BTRC antibody  >2 publications with CDC25A and BTRC
 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram
 Cyclin A:Cdk2-associated Events At S Phase Entry Pathway antibodies  Cyclin A:Cdk2-associated Events At S Phase Entry Pathway Diagram
 Cyclin B2 Mediated Events Pathway antibodies  Cyclin B2 Mediated Events Pathway Diagram
Diseases associated with anti-CDC25A antibodyOrgans/Tissues associated with anti-CDC25A antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >85 publications with CDC25A and Breast Neoplasms
 Adenocarcinoma Antibodies  >82 publications with CDC25A and Adenocarcinoma
 Liver Diseases Antibodies  >53 publications with CDC25A and Liver Diseases
 Neoplasms, Experimental Antibodies  >46 publications with CDC25A and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >45 publications with CDC25A and Lung Neoplasms
 Liver Neoplasms Antibodies  >43 publications with CDC25A and Liver Neoplasms
 Lung Diseases Antibodies  >40 publications with CDC25A and Lung Diseases
 Carcinoma, Hepatocellular Antibodies  >39 publications with CDC25A and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >32 publications with CDC25A and Disease Models, Animal
 Nervous System Diseases Antibodies  >28 publications with CDC25A and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >82 publications with CDC25A and Liver
 Lung Antibodies  >74 publications with CDC25A and Lung
 Blood Antibodies  >68 publications with CDC25A and Blood
 Prostate Antibodies  >54 publications with CDC25A and Prostate
 Brain Antibodies  >40 publications with CDC25A and Brain
 Muscle Antibodies  >29 publications with CDC25A and Muscle
 Skin Antibodies  >26 publications with CDC25A and Skin
 Embryonic Tissue Antibodies  >25 publications with CDC25A and Embryonic Tissue
 Testis Antibodies  >24 publications with CDC25A and Testis
 Bone Antibodies  >23 publications with CDC25A and Bone
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions