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anti-CDT1 antibody :: Rabbit CDT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9608602
Western Blot (WB)
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CDT1, Polyclonal Antibody

★Popular Item★
 Also Known As   

CDT1 Antibody

 Product Synonym Names    CDT 1; cdt1; CDT1_HUMAN; Chromatin licensing and DNA replication factor 1; DNA replication factor; DNA replication factor Cdt1; Double parked; Double parked Drosophila homolog of; Double parked homolog; DUP; Retroviral integration site 1; Retroviral integration site 2; Retroviral integration site1; Retroviral integration site2; RIS 2; RIS2
 Product Gene Name   

anti-CDT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q9H211
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    CDT1 antibody detects endogenous levels of total CDT1
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human CDT1
 Subcellular Location    Nucleus.
 Tissue Specificity    Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-CDT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CDT1 antibody

   Function: Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212, PubMed:26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity).
Subunit Structure: Interacts with GMNN; inhibits binding of the MCM complex to origins of replication (PubMed:11125146, PubMed:14672932, PubMed:14993212, PubMed:15257290). Interacts with CDC6; are mutually dependent on one another for loading MCM complexes onto chromatin (PubMed:14672932). Interacts with PCNA (PubMed:16861906). Interacts with LRWD1 during G1 phase and during mitosis (PubMed:22645314). Interacts with NDC80 subunit of the NDC80 complex; leading to kinetochore localization (PubMed:22581055). Interacts with GRWD1; origin binding of GRWD1 is dependent on CDT1 (PubMed:25990725). Interacts with KAT7 (PubMed:18832067). Interacts with ubiquitin-binding protein FAF1; the interaction is likely to promote CDT1 degradation (PubMed:26842564).
Post-translational Modifications: Two independent E3 ubiquitin ligase complexes, SCF(SKP2) and the DCX(DTL) complex, mediated CDT1 degradation in S phase. Ubiquitinated by the DCX(DTL) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Phosphorylation at Thr-29 by CDK2 targets CDT1 for ubiquitination by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). The interaction with GMNN protects it against ubiquitination. Deubiquitinated by USP37 (PubMed:27296872). Phosphorylation by cyclin A-dependent kinases at Thr-29 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). Phosphorylated at Thr-29 by MAPK8/JNK1, which blocks replication licensing in response to stress (PubMed:21856198). Binding to GMNN is not affected by phosphorylation.
Similarity: The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination. Belongs to the Cdt1 family.
 Applications Tested/Suitable for anti-CDT1 antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-CDT1 antibody    WB: 1:1000-3000
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 Western Blot (WB) of anti-CDT1 antibody    Western blot analysis of CDT1 using LOVO whole cell lysates
anti-CDT1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for CDT1. It may not necessarily be applicable to this product.
 NCBI GI #    188497689
 NCBI GeneID    81620
 NCBI Accession #    NP_112190.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_030928.3 [Other Products]
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 UniProt Primary Accession #    Q9H211 [Other Products]
 UniProt Secondary Accession #    Q86XX9; Q96CJ5; Q96GK5; Q96H67; Q96HE6; Q9BWM0 [Other Products]
 UniProt Related Accession #    Q9H211 [Other Products]
 Molecular Weight    Observed: 63 kDa
Predicted: 61 kDa
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 NCBI Official Full Name    DNA replication factor Cdt1
 NCBI Official Synonym Full Names    chromatin licensing and DNA replication factor 1
 NCBI Official Symbol    CDT1 [Similar Products]
 NCBI Official Synonym Symbols   
DUP; RIS2
[Similar Products]
 NCBI Protein Information    DNA replication factor Cdt1
 UniProt Protein Name    DNA replication factor Cdt1
 UniProt Synonym Protein Names   
Double parked homolog; DUP
 Protein Family    CDT1-like protein
 UniProt Gene Name    CDT1 [Similar Products]
 UniProt Synonym Gene Names    DUP [Similar Products]
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 NCBI Summary for CDT1    The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]
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 UniProt Comments for CDT1    Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212, PubMed:26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC)(PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene ().
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 Research Articles on CDT1    1. Results show that both Cdc6 and Cdt1, when expressed in a high level, alone or in combination, were significantly associated with poorer survival in the breast cancer patient cohort. In line with this finding, the expression of Cdc6 and Cdt1 was upregulated in breast cancer cells compared to normal breast epithelial cells. Expression of Cdc6 and Cdt1 was significantly higher in ER negative breast cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CDT1 antibodyPathways associated with anti-CDT1 antibody
 Reference Product  PubMed Publications
 CDC6 antibody  >121 publications with CDT1 and CDC6
 GMNN antibody  >103 publications with CDT1 and GMNN
 MCM2 antibody  >85 publications with CDT1 and MCM2
 PCNA antibody  >51 publications with CDT1 and PCNA
 MCM7 antibody  >28 publications with CDT1 and MCM7
 CDK2 antibody  >26 publications with CDT1 and CDK2
 CDC45 antibody  >22 publications with CDT1 and CDC45
 MCM6 antibody  >19 publications with CDT1 and MCM6
 MCM4 antibody  >17 publications with CDT1 and MCM4
 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 CDT1 Association With The CDC6:ORC:origin Complex Pathway antibodies  CDT1 Association With The CDC6:ORC:origin Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram
Diseases associated with anti-CDT1 antibodyOrgans/Tissues associated with anti-CDT1 antibody
 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >9 publications with CDT1 and Cell Transformation, Neoplastic
 Meier-Gorlin syndrome Antibodies  >7 publications with CDT1 and Meier-Gorlin syndrome
 Breast Neoplasms Antibodies  >6 publications with CDT1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >5 publications with CDT1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with CDT1 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >3 publications with CDT1 and Precancerous Conditions
 Lung Neoplasms Antibodies  >3 publications with CDT1 and Lung Neoplasms
 Hyperplasia Antibodies  >3 publications with CDT1 and Hyperplasia
 Disease Models, Animal Antibodies  >2 publications with CDT1 and Disease Models, Animal
 Adenocarcinoma Antibodies  >2 publications with CDT1 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >10 publications with CDT1 and Bone
 Brain Antibodies  >10 publications with CDT1 and Brain
 Blood Antibodies  >10 publications with CDT1 and Blood
 Lung Antibodies  >9 publications with CDT1 and Lung
 Ovary Antibodies  >7 publications with CDT1 and Ovary
 Eye Antibodies  >6 publications with CDT1 and Eye
 Prostate Antibodies  >5 publications with CDT1 and Prostate
 Bone Marrow Antibodies  >4 publications with CDT1 and Bone Marrow
 Embryonic Tissue Antibodies  >4 publications with CDT1 and Embryonic Tissue
 Kidney Antibodies  >3 publications with CDT1 and Kidney
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