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anti-MB21D1 antibody :: Rabbit anti-Human CF150 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9214999
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CF150 (MB21D1), Polyclonal Antibody

★Popular Item★
 Also Known As   

CF150 Antibody (Center)

 Product Synonym Names    Cyclic GMP-AMP synthase; cGAMP synthase; cGAS; h-cGAS; Mab-21 domain-containing protein 1; MB21D1; C6orf150
 Product Gene Name   

anti-MB21D1 antibody

[Similar Products]
 Antibody/Peptide Pairs    CF150 peptide (MBS9217648) is used for blocking the activity of CF150 antibody (MBS9214999)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    266-295
 OMIM    613973
 3D Structure    ModBase 3D Structure for Q8N884
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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 Cellular Location    Cytoplasm, cytosol Note=Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1)(PubMed:26048138)
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 Preparation and Storage    Maintain refrigerated at 2-8░C for up to 2 weeks. For long term storage store at -20░C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-MB21D1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MB21D1 antibody

   The exact function of C6orf150 remains unknown.
 Product Categories/Family for anti-MB21D1 antibody    Cell Biology
 Applications Tested/Suitable for anti-MB21D1 antibody   

Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)

 Application Notes for anti-MB21D1 antibody    FC~~1:10~50
WB~~1:500-1:1000
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 Flow Cytometry (FC/FACS) of anti-MB21D1 antibody    Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). MBS9214999 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
anti-MB21D1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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 Western Blot (WB) of anti-MB21D1 antibody    Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
anti-MB21D1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-MB21D1 antibody    Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
anti-MB21D1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-MB21D1 antibody    Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody (Center). MBS9214999 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
anti-MB21D1 antibody Western Blot (WB) (WB) image
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 Flow Cytometry (FC/FACS) of anti-MB21D1 antibody    CF150 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-MB21D1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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 Testing Data(TD) of anti-MB21D1 antibody    Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37║C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight« 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37║C. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed
anti-MB21D1 antibody Testing Data(TD) image
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NCBI/Uniprot data below describe general gene information for MB21D1. It may not necessarily be applicable to this product.
 NCBI GI #    115511030
 NCBI GeneID    115004
 NCBI Accession #    NP_612450.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_138441.2 [Other Products]
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 UniProt Primary Accession #    Q8N884 [Other Products]
 UniProt Secondary Accession #    Q14CV6; Q32NC9; Q5SWL0; Q5SWL1; Q96E45; L0L2J9 [Other Products]
 UniProt Related Accession #    Q8N884 [Other Products]
 Molecular Weight    58814
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 NCBI Official Full Name    cyclic GMP-AMP synthase
 NCBI Official Synonym Full Names    Mab-21 domain containing 1
 NCBI Official Symbol    MB21D1 [Similar Products]
 NCBI Official Synonym Symbols   
cGAS; h-cGAS; C6orf150
[Similar Products]
 NCBI Protein Information    cyclic GMP-AMP synthase
 UniProt Protein Name    Cyclic GMP-AMP synthase
 UniProt Synonym Protein Names   
Mab-21 domain-containing protein 1
 Protein Family    Cyclic GMP-AMP synthase
 UniProt Gene Name    MB21D1 [Similar Products]
 UniProt Synonym Gene Names    C6orf150; cGAMP synthase; cGAS; h-cGAS [Similar Products]
 UniProt Entry Name    CGAS_HUMAN
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 UniProt Comments for MB21D1    MB21D1: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Protein type: Enzyme, cellular metabolism; EC 2.7.7.86

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: cytosol

Molecular Function: GTP binding; DNA binding; metal ion binding; ATP binding

Biological Process: cyclic nucleotide biosynthetic process; positive regulation of interferon type I production; innate immune response; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus
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Product References and Citations for anti-MB21D1 antibody

   Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
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 Research Articles on MB21D1    1. our study not only provides a novel mechanism of modulating cGAS expression, but also adds another layer of regulation in DNA-triggered IFN-I production by induction of cGAS.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MB21D1 antibodyPathways associated with anti-MB21D1 antibody
 Reference Product  PubMed Publications
 TMEM173 antibody  >3 publications with MB21D1 and TMEM173
 DDX41 antibody  >2 publications with MB21D1 and DDX41
 TREX1 antibody  >2 publications with MB21D1 and TREX1
 MOV10 antibody  >1 publications with MB21D1 and MOV10
 FAM46C antibody  >1 publications with MB21D1 and FAM46C
 MCOLN2 antibody  >1 publications with MB21D1 and MCOLN2
 DDX60 antibody  >1 publications with MB21D1 and DDX60
 Products by Pathway  Pathway Diagram
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 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
Diseases associated with anti-MB21D1 antibodyOrgans/Tissues associated with anti-MB21D1 antibody
 Disease Name  Pubmed Publications
 Neoplasms, Experimental Antibodies  >2 publications with MB21D1 and Neoplasms, Experimental
 Mitochondrial Diseases Antibodies  >1 publications with MB21D1 and Mitochondrial Diseases
 Inflammation Antibodies  >1 publications with MB21D1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with MB21D1 and Blood
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