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anti-CHFR antibody :: Rabbit anti-Human, Mouse CHFR Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7050827
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CHFR, Polyclonal Antibody

★Popular Item★
 Also Known As   

CHFR Antibody

 Product Synonym Names    E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196; CHFR; RNF196
 Product Gene Name   

anti-CHFR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    AF170724 mRNA
 3D Structure    ModBase 3D Structure for Q96EP1
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Immunogen    Recombinant human E3 ubiquitin-protein ligase CHFR protein (141-422AA)
 Preservative    0.03% Proclin 300
 Constituents    50% Glycerol, 0.01M PBS, PH 7.4
 Conjugation    Non-conjugated
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-13287 / sc-13288 / sc-13290 / sc-28263
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-CHFR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CHFR antibody

   E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
 Applications Tested/Suitable for anti-CHFR antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-CHFR antibody    WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
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 Western Blot (WB) of anti-CHFR antibody    Western Blot
Positive WB detected in:HepG2 whole cell lysate,Hela whole cell lysate,Jurkat whole cell lysate,Mouse heart tissue
All lanes: CHFR antibody at 3.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000000 dilution
Predicted band size: 74,73,70,64 kDa
Observed band size: 74 kDa

anti-CHFR antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-CHFR antibody    Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7050827 at dilution of 1:100
anti-CHFR antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CHFR antibody    Immunohistochemistry of paraffin-embedded human lung cancer using MBS7050827 at dilution of 1:100
anti-CHFR antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CHFR. It may not necessarily be applicable to this product.
 NCBI GI #    239048792
 NCBI GeneID    55743
 NCBI Accession #    NP_001154816.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001161344.1 [Other Products]
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 UniProt Primary Accession #    Q96EP1 [Other Products]
 UniProt Secondary Accession #    Q96SL3; Q9NRT4; Q9NT32; Q9NVD5; A6NEN5; B4DZ77; B4E2L6 [Other Products]
 UniProt Related Accession #    Q96EP1 [Other Products]
 Molecular Weight    63,873 Da
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 NCBI Official Full Name    E3 ubiquitin-protein ligase CHFR isoform 1
 NCBI Official Synonym Full Names    checkpoint with forkhead and ring finger domains
 NCBI Official Symbol    CHFR [Similar Products]
 NCBI Official Synonym Symbols   
RNF116; RNF196
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase CHFR
 UniProt Protein Name    E3 ubiquitin-protein ligase CHFR
 UniProt Synonym Protein Names   
Checkpoint with forkhead and RING finger domains protein; RING finger protein 196
 Protein Family    E3 ubiquitin-protein ligase
 UniProt Gene Name    CHFR [Similar Products]
 UniProt Synonym Gene Names    RNF196 [Similar Products]
 UniProt Entry Name    CHFR_HUMAN
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 NCBI Summary for CHFR    This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
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 UniProt Comments for CHFR    CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: nucleus; PML body

Molecular Function: nucleotide binding; protein binding; ubiquitin-protein ligase activity

Biological Process: mitotic cell cycle checkpoint; modification-dependent protein catabolic process; protein polyubiquitination; ubiquitin-dependent protein catabolic process
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 Research Articles on CHFR    1. Data show that hypermethylation of the CHFR promoter, frequent in acute myeloid leukemia, is associated with adverse outcome, and can thus be used for risk stratification.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CHFR antibodyDiseases associated with anti-CHFR antibody
 Reference Product  PubMed Publications
 PLK1 antibody  >10 publications with CHFR and PLK1
 AURKA antibody  >5 publications with CHFR and AURKA
 HLTF antibody  >4 publications with CHFR and HLTF
 PARP1 antibody  >4 publications with CHFR and PARP1
 USP7 antibody  >2 publications with CHFR and USP7
 APLF antibody  >1 publications with CHFR and APLF
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >65 publications with CHFR and Carcinoma
 Stomach Neoplasms Antibodies  >29 publications with CHFR and Stomach Neoplasms
 Adenocarcinoma Antibodies  >25 publications with CHFR and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >23 publications with CHFR and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >17 publications with CHFR and Lung Neoplasms
 Lung Diseases Antibodies  >17 publications with CHFR and Lung Diseases
 Neoplasms, Experimental Antibodies  >12 publications with CHFR and Neoplasms, Experimental
 Carcinoma, Non-Small-Cell Lung Antibodies  >12 publications with CHFR and Carcinoma, Non-Small-Cell Lung
 Uterine Neoplasms Antibodies  >10 publications with CHFR and Uterine Neoplasms
 Endometrial Neoplasms Antibodies  >6 publications with CHFR and Endometrial Neoplasms
Organs/Tissues associated with anti-CHFR antibody
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >20 publications with CHFR and Lung
 Blood Antibodies  >11 publications with CHFR and Blood
 Lymph Node Antibodies  >9 publications with CHFR and Lymph Node
 Mouth Antibodies  >5 publications with CHFR and Mouth
 Muscle Antibodies  >4 publications with CHFR and Muscle
 Uterus Antibodies  >3 publications with CHFR and Uterus
 Liver Antibodies  >3 publications with CHFR and Liver
 Eye Antibodies  >3 publications with CHFR and Eye
 Skin Antibodies  >2 publications with CHFR and Skin
 Prostate Antibodies  >2 publications with CHFR and Prostate
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