Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow CHUK rightarrow Polyclonal CHUK  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • TBCE Antibody
 • Lactogen Placental (LTF) Antibody
 • Prostaglandin E2 (PGE2) ELISA Kit
 • D4 GDI Antibody
 • Vasoactive intestinal Polypeptide Receptor 2 (VIPR2) Antibody
 • NDUFV2 Antibody
 • Thioredoxin Reductase (TRXR) Recombinant Protein
 • Apolipoprotein B (APOB) Recombinant Protein
 • Periostin-OSF2 (POSTN) ELISA Kit
 • Paired box protein Pax-4 (PAX4) Recombinant Protein
 • CD44 Antibody
 • Myc proto-oncogene protein (MYC) Recombinant Protein
 • MMP24 Antibody
 • Phosphatidylethanolamine Antibody (IgG) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-CHUK antibody :: Rabbit CHUK Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9212858
Western Blot (WB)
Unit / Price
0.08 mL  /  $155 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CHUK, Polyclonal Antibody

 Also Known As   

CHUK Antibody (N-term)

 Product Synonym Names    Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA; TCF16
 Product Gene Name   

anti-CHUK antibody

[Similar Products]
 Antibody/Peptide Pairs    CHUK peptide (MBS9228598) is used for blocking the activity of CHUK antibody (MBS9212858)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Immunogen Sequence Positions    84-112
 OMIM    600664
 3D Structure    ModBase 3D Structure for O15111
Section Bar
 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Clone Number    RB41290
 Host    Rabbit
 Species Reactivity    Human (Predicted Reactivity: Bovine, Chicken, Mouse)
Section Bar
 Specificity    This CHUK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human CHUK.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.5 (lot specific)
Section Bar
 Function    Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non -canonicalNF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.
 Cellular Location    Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus
 Tissue Location    Widely expressed.
Section Bar
 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-CHUK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-CHUK antibody

   This gene encodes a member of the serine/threonine protein
kinase family. The encoded protein, a component of a
cytokine-activated protein complex that is an inhibitor of the
essential transcription factor NF-kappa-B complex, phosphorylates
sites that trigger the degradation of the inhibitor via the
ubiquination pathway, thereby activating the transcription factor.
 Product Categories/Family for anti-CHUK antibody    Cardiovascular; Cell Biology; Immunology; Signal Transduction
 Applications Tested/Suitable for anti-CHUK antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-CHUK antibody    WB~~1:1000
Section Bar
 Western Blot (WB) of anti-CHUK antibody    CHUK Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CHUK antibody detected the CHUK protein (arrow).
anti-CHUK antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for CHUK. It may not necessarily be applicable to this product.
 NCBI GI #    62241001
 NCBI GeneID    1147
 NCBI Accession #    NP_001269.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001278.3 [Other Products]
Section Bar
 UniProt Primary Accession #    O15111 [Other Products]
 UniProt Secondary Accession #    O14666; Q13132; Q5W0I4; Q92467 [Other Products]
 UniProt Related Accession #    O15111 [Other Products]
 Molecular Weight    84640
Table BarTOPTable Bar
 NCBI Official Full Name    inhibitor of nuclear factor kappa-B kinase subunit alpha
 NCBI Official Synonym Full Names    conserved helix-loop-helix ubiquitous kinase
 NCBI Official Symbol    CHUK [Similar Products]
 NCBI Official Synonym Symbols   
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
[Similar Products]
 NCBI Protein Information    inhibitor of nuclear factor kappa-B kinase subunit alpha
 UniProt Protein Name    Inhibitor of nuclear factor kappa-B kinase subunit alpha
 UniProt Synonym Protein Names   
Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16
 Protein Family    Inhibitor of nuclear factor kappa-B kinase
 UniProt Gene Name    CHUK [Similar Products]
 UniProt Synonym Gene Names    IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16 [Similar Products]
 UniProt Entry Name    IKKA_HUMAN
Section Bar
 NCBI Summary for CHUK    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for CHUK    IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Kinase, protein; Protein kinase, Other; Other group; IKK family

Chromosomal Location of Human Ortholog: 10q24-q25

Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity

Biological Process: lactation; skeletal muscle contraction; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; osteoclast differentiation; protein amino acid phosphorylation; T cell receptor signaling pathway; Rho protein signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; morphogenesis of an epithelial sheet; inflammatory response; toll-like receptor 4 signaling pathway; response to drug; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; response to virus; MyD88-independent toll-like receptor signaling pathway; mammary gland epithelial cell proliferation; response to amino acid stimulus; odontogenesis of dentine-containing teeth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; innate immune response; striated muscle cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation

Disease: Cocoon Syndrome
Table BarTOPTable Bar
 

Product References and Citations for anti-CHUK antibody

   Lahtela, J., et al. N. Engl. J. Med. 363(17):1631-1637(2010)
Gouin, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(40):17333-17338(2010)
Cummins, E.P., et al. J. Immunol. 185(7):4439-4445(2010)
Li, T., et al. Nat. Immunol. 11(9):799-805(2010)
Rotman, Y., et al. Hepatology 52(3):894-903(2010)
Section Bar
 Research Articles on CHUK    1. Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-CHUK antibodyPathways associated with anti-CHUK antibody
 Reference Product  PubMed Publications
 IKBKB antibody  >1189 publications with CHUK and IKBKB
 RELA antibody  >160 publications with CHUK and RELA
 TRAF2 antibody  >69 publications with CHUK and TRAF2
 AKT1 antibody  >39 publications with CHUK and AKT1
 NFKB2 antibody  >21 publications with CHUK and NFKB2
 IKBKG antibody  >16 publications with CHUK and IKBKG
 NFKB1 antibody  >14 publications with CHUK and NFKB1
 NFKBIA antibody  >5 publications with CHUK and NFKBIA
 NFKBIB antibody  >1 publications with CHUK and NFKBIB
 MAP4K4 antibody  >1 publications with CHUK and MAP4K4
 Products by Pathway  Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
 Glycine, Serine And Threonine Metabolism Pathway antibodies  Glycine, Serine And Threonine Metabolism Pathway Diagram
Diseases associated with anti-CHUK antibodyOrgans/Tissues associated with anti-CHUK antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >485 publications with CHUK and Necrosis
 Inflammation Antibodies  >242 publications with CHUK and Inflammation
 Skin Abnormalities Antibodies  >39 publications with CHUK and Skin Abnormalities
 Abnormalities, Multiple Antibodies  >36 publications with CHUK and Abnormalities, Multiple
 Heart Diseases Antibodies  >36 publications with CHUK and Heart Diseases
 Breast Neoplasms Antibodies  >29 publications with CHUK and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >28 publications with CHUK and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >25 publications with CHUK and Liver Diseases
 Adenocarcinoma Antibodies  >20 publications with CHUK and Adenocarcinoma
 Liver Neoplasms Antibodies  >15 publications with CHUK and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >234 publications with CHUK and Blood
 Lung Antibodies  >72 publications with CHUK and Lung
 Skin Antibodies  >65 publications with CHUK and Skin
 Liver Antibodies  >60 publications with CHUK and Liver
 Bone Antibodies  >54 publications with CHUK and Bone
 Heart Antibodies  >53 publications with CHUK and Heart
 Muscle Antibodies  >44 publications with CHUK and Muscle
 Kidney Antibodies  >39 publications with CHUK and Kidney
 Embryonic Tissue Antibodies  >35 publications with CHUK and Embryonic Tissue
 Brain Antibodies  >29 publications with CHUK and Brain
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions