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anti-CLOCK antibody :: Rabbit anti-Human CLOCK Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7051869
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CLOCK, Polyclonal Antibody

★Popular Item★
 Also Known As   

CLOCK Antibody

 Product Synonym Names    Circadian locomoter output cycles protein kaput; hCLOCK; Class E basic helix-loop-helix protein 8; bHLHe8; CLOCK; BHLHE8; KIAA0334
 Product Gene Name   

anti-CLOCK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    AF011568 mRNA
 3D Structure    ModBase 3D Structure for O15516
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Immunogen    Recombinant Human Circadian locomoter output cycles protein kaput protein (577-846AA)
 Conjugation    Non-conjugated
 Preservative    0.03% Proclin 300
 Constituents    50% Glycerol, 0.01M PBS, pH 7.4
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-CLOCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CLOCK antibody

   Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and nonhistone proteins, including its own partner ARNTL/BMAL1. Regulates the circadian expression of ICAM1, VCAM1, CCL2, THPO and MPL and also acts as an enhancer of the transactivation potential of NF-kappaB. Plays an important role in the homeostatic regulation of sleep. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The CLOCK-ARNTL2/BMAL2 heterodimer activates the transcription of SERPINE1/PAI1 and BHLHE40/DEC1.
 Applications Tested/Suitable for anti-CLOCK antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-CLOCK antibody    WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
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 Western Blot (WB) of anti-CLOCK antibody    Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: CLOCK antibody at 3.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 96 KDa
Observed band size: 96 KDa

anti-CLOCK antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-CLOCK antibody    Immunohistochemistry of paraffin-embedded human brain tissue using MBS7051869 at dilution of 1:100
anti-CLOCK antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CLOCK antibody    Immunofluorescent analysis of Hela cells using MBS7051869 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-CLOCK antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CLOCK. It may not necessarily be applicable to this product.
 NCBI GI #    392841204
 NCBI GeneID    9575
 NCBI Accession #    NP_001254772.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001267843.1 [Other Products]
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 UniProt Primary Accession #    O15516 [Other Products]
 UniProt Secondary Accession #    O14516; Q9UIT8; A0AV01; A2I2N9 [Other Products]
 UniProt Related Accession #    O15516 [Other Products]
 Molecular Weight    95,304 Da
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 NCBI Official Full Name    circadian locomoter output cycles protein kaput
 NCBI Official Synonym Full Names    clock circadian regulator
 NCBI Official Symbol    CLOCK [Similar Products]
 NCBI Official Synonym Symbols   
KAT13D; bHLHe8
[Similar Products]
 NCBI Protein Information    circadian locomoter output cycles protein kaput
 UniProt Protein Name    Circadian locomoter output cycles protein kaput
 UniProt Synonym Protein Names   
Class E basic helix-loop-helix protein 8; bHLHe8
 Protein Family    Clock-controlled protein
 UniProt Gene Name    CLOCK [Similar Products]
 UniProt Synonym Gene Names    BHLHE8; KIAA0334; hCLOCK; bHLHe8 [Similar Products]
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 NCBI Summary for CLOCK    The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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 UniProt Comments for CLOCK    CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: Acetyltransferase; DNA-binding; EC 2.3.1.48; Transcription factor

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: chromosome; intracellular membrane-bound organelle; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; DNA binding; histone acetyltransferase activity; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: activation of NF-kappaB transcription factor; circadian regulation of gene expression; circadian rhythm; DNA damage checkpoint; negative regulation of transcription, DNA-dependent; photoperiodism; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of hair cycle; regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to redox state; signal transduction; spermatogenesis
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 Research Articles on CLOCK    1. The possession of CLOCK rs3749474 may influence the effect of reducing the percentage intake of dietary fat on obesity-associated variables and carrying this SNP might benefit more than others from weight loss treatment involving dietary fat restriction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CLOCK antibodyPathways associated with anti-CLOCK antibody
 Reference Product  PubMed Publications
 ARNTL antibody  >641 publications with CLOCK and ARNTL
 PER3 antibody  >206 publications with CLOCK and PER3
 ARNTL2 antibody  >30 publications with CLOCK and ARNTL2
 Products by Pathway  Pathway Diagram
 BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway antibodies  BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Circadian Repression Of Expression By REV-ERBA Pathway antibodies  Circadian Repression Of Expression By REV-ERBA Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 Dopaminergic Synapse Pathway antibodies  Dopaminergic Synapse Pathway Diagram
 Dopaminergic Synapse Pathway antibodies  Dopaminergic Synapse Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Diseases associated with anti-CLOCK antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >550 publications with CLOCK and Cardiovascular Diseases
 Neoplasms Antibodies  >482 publications with CLOCK and Neoplasms
 Cognition Disorders Antibodies  >366 publications with CLOCK and Cognition Disorders
 Heart Diseases Antibodies  >206 publications with CLOCK and Heart Diseases
 Neurobehavioral Manifestations Antibodies  >183 publications with CLOCK and Neurobehavioral Manifestations
 Drug Toxicity Antibodies  >134 publications with CLOCK and Drug Toxicity
 Inflammation Antibodies  >117 publications with CLOCK and Inflammation
 Liver Diseases Antibodies  >104 publications with CLOCK and Liver Diseases
 Myocardial Infarction Antibodies  >85 publications with CLOCK and Myocardial Infarction
 Carcinoma Antibodies  >77 publications with CLOCK and Carcinoma
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