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anti-CREB1 antibody :: Rabbit anti-Rat CREB (Ser133) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502016
Testing Data
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CREB (Ser133) (CREB1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Phospho-Ser133 CREB

 Product Gene Name   

anti-CREB1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_005108.3 (71277171..71346198, complement). Location: 9q32
 3D Structure    ModBase 3D Structure for P15337
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Rat
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 Specificity    Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
 Immunogen Information    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-CREB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CREB1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-CREB1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-CREB1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:100
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 Testing Data of anti-CREB1 antibody    anti-CREB1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CREB1. It may not necessarily be applicable to this product.
 NCBI GI #    13591924
 NCBI GeneID    81646
 NCBI Accession #    NP_112279.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031017.1 [Other Products]
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 UniProt Primary Accession #    P15337 [Other Products]
 UniProt Related Accession #    P15337 [Other Products]
 Molecular Weight    45
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 NCBI Official Full Name    cyclic AMP-responsive element-binding protein 1 isoform B
 NCBI Official Synonym Full Names    cAMP responsive element binding protein 1
 NCBI Official Symbol    Creb1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1
 UniProt Protein Name    Cyclic AMP-responsive element-binding protein 1
 Protein Family    Cyclic AMP-responsive element-binding protein
 UniProt Gene Name    Creb1 [Similar Products]
 UniProt Synonym Gene Names    Creb-1; CREB-1 [Similar Products]
 UniProt Entry Name    CREB1_RAT
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 NCBI Summary for CREB1    binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]
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 UniProt Comments for CREB1    Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells

By similarity.

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3

By similarity. When phosphorylated on Ser-133, binds CREBBP. Interacts with ARRB1. Interacts (phosphorylated form) with TOX3. Binds to HIPK2

By similarity. Interacts with SGK1

By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity

By similarity.

Subcellular location: Nucleus.

Post-translational modification: Phosphorylation of Ser-133 allows CREBBP binding. Stimulated by phosphorylation. Phosphorylation of both Ser-142 and Ser-133 in the SCN regulates the activity of CREB and participate in circadian rhythm generation

By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli

By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity

By similarity. Ref.3 Ref.4 Ref.5Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

By similarity.

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.
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Product References and Citations for anti-CREB1 antibody

   • Johannessen M, Delghandi MP, Moens U (2004) What turns CREB on Cellular Signalling 16:1211-1227. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr Biol 5:747-757.
• Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233.
• Montminy M (1997) Transcriptional regulation by cyclic AMP. Annu Rev Biochem 66:807-822.
• Shaywitz AJ, Greenberg ME (1999) CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861.
• Silva AJ, Kogan JH, Frankland PW, Kida S (1998) CREB and memory. Annu Rev Neurosci 21:127-148.
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 Research Articles on CREB1    1. negative regulation of CREB activity by endogenous CaMKIIdelta-dependent CREB-Ser(142) phosphorylation and suggest a potential mechanism for CaMKIIdelta/CREB signaling in modulating proliferation and migration in VSM cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CREB1 antibodyPathways associated with anti-CREB1 antibody
 Reference Product  PubMed Publications
 Fos antibody  >47 publications with CREB1 and Fos
 Mapk1 antibody  >34 publications with CREB1 and Mapk1
 Mapk3 antibody  >33 publications with CREB1 and Mapk3
 Crebbp antibody  >18 publications with CREB1 and Crebbp
 Ep300 antibody  >6 publications with CREB1 and Ep300
 Akt1 antibody  >6 publications with CREB1 and Akt1
 Camk4 antibody  >5 publications with CREB1 and Camk4
 Camk2b antibody  >2 publications with CREB1 and Camk2b
 Ciita antibody  >2 publications with CREB1 and Ciita
 Nr3c1 antibody  >1 publications with CREB1 and Nr3c1
 Products by Pathway  Pathway Diagram
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 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
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 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
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Diseases associated with anti-CREB1 antibodyOrgans/Tissues associated with anti-CREB1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >181 publications with CREB1 and Neoplasms
 Nervous System Diseases Antibodies  >99 publications with CREB1 and Nervous System Diseases
 Inflammation Antibodies  >59 publications with CREB1 and Inflammation
 Necrosis Antibodies  >40 publications with CREB1 and Necrosis
 Depressive Disorder Antibodies  >39 publications with CREB1 and Depressive Disorder
 Memory Disorders Antibodies  >38 publications with CREB1 and Memory Disorders
 Learning Disorders Antibodies  >29 publications with CREB1 and Learning Disorders
 Atherosclerosis Antibodies  >19 publications with CREB1 and Atherosclerosis
 Liver Diseases Antibodies  >16 publications with CREB1 and Liver Diseases
 Cognition Disorders Antibodies  >16 publications with CREB1 and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >257 publications with CREB1 and Brain
 Heart Antibodies  >30 publications with CREB1 and Heart
 Embryonic Tissue Antibodies  >22 publications with CREB1 and Embryonic Tissue
 Pancreas Antibodies  >12 publications with CREB1 and Pancreas
 Testis Antibodies  >12 publications with CREB1 and Testis
 Thymus Antibodies  >4 publications with CREB1 and Thymus
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