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anti-CRKL antibody :: Goat CRKL Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420280
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CRKL, Polyclonal Antibody

 Also Known As   

Goat anti-CRKL Antibody

 Product Synonym Names    CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v-crk avian sarcoma virus CT10 oncogene homolog-like; OTTHUMP00000198539; CRKL antibody; v-crk sarcoma virus CT10 oncogene homolog (avian)-like antibody; v-crk avian sarcoma virus CT10 oncogene homolog-like antibody; OTTHUMP00000198539 antibody
 Product Gene Name   

anti-CRKL antibody

[Similar Products]
 Antibody/Peptide Pairs    CRKL peptide (MBS425568) is used for blocking the activity of CRKL antibody (MBS420280)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    KIFDPQNPDE NE
 OMIM    602007
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.
 Epitope    C Terminus
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-CRKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CRKL antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-CRKL antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx 37kDa band observed in lysates of cell lines K562, Jurkat, NIH3T3, and of Rat Spleen (calculated MW of 33.8kDa according to NP_005198). Recommended concentration: 0.01-0.03ug/ml.
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 Western Blot (WB) of anti-CRKL antibody    (0. 01ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CRKL antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-CRKL antibody    (3.8ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-CRKL antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CRKL. It may not necessarily be applicable to this product.
 NCBI GI #    4885153
 NCBI GeneID    1399
 NCBI Accession #    NP_005198.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005207.3 [Other Products]
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 UniProt Secondary Accession #    A8KA44; D3DX35 [Other Products]
 UniProt Related Accession #    P46109 [Other Products]
 Molecular Weight    33,777 Da
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 NCBI Official Full Name    crk-like protein
 NCBI Official Synonym Full Names    v-crk avian sarcoma virus CT10 oncogene homolog-like
 NCBI Official Symbol    CRKL [Similar Products]
 NCBI Protein Information    crk-like protein
 UniProt Protein Name    Crk-like protein
 Protein Family    Crk-like protein
 UniProt Gene Name    CRKL [Similar Products]
 UniProt Entry Name    CRKL_HUMAN
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 NCBI Summary for CRKL    This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
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 UniProt Comments for CRKL    CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: protein binding; SH3/SH2 adaptor activity; signal transducer activity

Biological Process: activation of MAPKK activity; anterior/posterior pattern formation; blood vessel development; heart development; JNK cascade; organ morphogenesis; parathyroid gland development; positive regulation of cell proliferation; Ras protein signal transduction; thymus development
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 Research Articles on CRKL    1. CrkL regulates CCL19 and CCR7-induced epithelial-to-mesenchymal transition via ERK signaling pathway in epithelial ovarian carcinoma patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CRKL antibodyPathways associated with anti-CRKL antibody
 Reference Product  PubMed Publications
 BCR antibody  >170 publications with CRKL and BCR
 CBL antibody  >71 publications with CRKL and CBL
 ABL1 antibody  >13 publications with CRKL and ABL1
 BCAR1 antibody  >10 publications with CRKL and BCAR1
 PXN antibody  >9 publications with CRKL and PXN
 GAB1 antibody  >7 publications with CRKL and GAB1
 NEDD9 antibody  >6 publications with CRKL and NEDD9
 MAP4K1 antibody  >1 publications with CRKL and MAP4K1
 Products by Pathway  Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
Diseases associated with anti-CRKL antibodyOrgans/Tissues associated with anti-CRKL antibody
 Disease Name  Pubmed Publications
 Leukemia, Myelogenous, Chronic, BCR-ABL Positive Antibodies  >97 publications with CRKL and Leukemia, Myelogenous, Chronic, BCR-ABL Positive
 Cell Transformation, Neoplastic Antibodies  >36 publications with CRKL and Cell Transformation, Neoplastic
 Cardiovascular Diseases Antibodies  >22 publications with CRKL and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >17 publications with CRKL and Lung Neoplasms
 DiGeorge Syndrome Antibodies  >15 publications with CRKL and DiGeorge Syndrome
 Lung Diseases Antibodies  >14 publications with CRKL and Lung Diseases
 Disease Models, Animal Antibodies  >13 publications with CRKL and Disease Models, Animal
 Leukemia, Myeloid, Acute Antibodies  >13 publications with CRKL and Leukemia, Myeloid, Acute
 Breast Neoplasms Antibodies  >12 publications with CRKL and Breast Neoplasms
 Carcinoma, Hepatocellular Antibodies  >8 publications with CRKL and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >107 publications with CRKL and Blood
 Bone Antibodies  >28 publications with CRKL and Bone
 Lung Antibodies  >24 publications with CRKL and Lung
 Bone Marrow Antibodies  >24 publications with CRKL and Bone Marrow
 Nerve Antibodies  >21 publications with CRKL and Nerve
 Brain Antibodies  >20 publications with CRKL and Brain
 Heart Antibodies  >15 publications with CRKL and Heart
 Embryonic Tissue Antibodies  >13 publications with CRKL and Embryonic Tissue
 Liver Antibodies  >12 publications with CRKL and Liver
 Lymph Node Antibodies  >9 publications with CRKL and Lymph Node
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