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anti-NKX2-5 antibody :: Goat CSX1/NKX2-5 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS422194
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

CSX1/NKX2-5, Polyclonal Antibody

 Also Known As   

Goat anti-CSX1/NKX2-5 Antibody

 Product Synonym Names    NKX2-5; NK2 transcription factor related, locus 5 (Drosophila); CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1; NK2 transcription factor homolog E; NK2 transcription factor related, locus 5; cardiac-specific homeo box; tinman paralog; NKX2-5 antibody; NK2 transcription factor related; locus 5 (Drosophila) antibody; CHNG5 antibody; CSX antibody; CSX1 antibody; NKX2.5 antibody; NKX2E antibody; NKX4-1 antibody; NK2 transcription factor homolog E antibody; NK2 transcription factor related; locus 5 antibody; cardiac-specific homeo box antibody; tinman paralog antibody; CSX1 / NKX2-5
 Product Gene Name   

anti-NKX2-5 antibody

[Similar Products]
 Product Synonym Gene Name    CSX1 [Similar Products]
 Antibody/Peptide Pairs    CSX1 / NKX2-5 peptide (MBS426485) is used for blocking the activity of CSX1/NKX2-5 antibody (MBS422194)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    PRAYSDPDPA KDPR
 OMIM    108900
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.
 Epitope    Internal region
 Note    This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8697
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-NKX2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-NKX2-5 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-NKX2-5 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 45kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res.1996 Nov;79(5): 920-9.; PMID: 8888684). Recommended concentration: 0.3-1ug/ml.
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 Western Blot (WB) of anti-NKX2-5 antibody    (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-NKX2-5 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NKX2-5. It may not necessarily be applicable to this product.
 NCBI GI #    4758090
 NCBI GeneID    1482
 NCBI Accession #    NP_004378.1 [Other Products]
 NCBI Related Accession #    NP_001159647.1; NP_001159648.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004387.3 [Other Products]
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 UniProt Secondary Accession #    A8K3K0; B4DNB6; E9PBU6 [Other Products]
 UniProt Related Accession #    P52952 [Other Products]
 Molecular Weight    11,681 Da
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 NCBI Official Full Name    homeobox protein Nkx-2.5 isoform 1
 NCBI Official Synonym Full Names    NK2 homeobox 5
 NCBI Official Symbol    NKX2-5 [Similar Products]
 NCBI Official Synonym Symbols   
CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1
[Similar Products]
 NCBI Protein Information    homeobox protein Nkx-2.5
 UniProt Protein Name    Homeobox protein Nkx-2.5
 UniProt Synonym Protein Names   
Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
 Protein Family    Homeobox protein
 UniProt Gene Name    NKX2-5 [Similar Products]
 UniProt Synonym Gene Names    CSX; NKX2.5; NKX2E [Similar Products]
 UniProt Entry Name    NKX25_HUMAN
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 NCBI Summary for NKX2-5    This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
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 UniProt Comments for NKX2-5    NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; protein binding; protein heterodimerization activity; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: adult heart development; atrial cardiac muscle cell development; BMP signaling pathway; cardiac muscle cell differentiation; cardiac muscle cell proliferation; cardiac muscle contraction; cardiac muscle morphogensis; cell differentiation; embryonic heart tube development; heart looping; heart morphogenesis; hemopoiesis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; pharyngeal system development; positive regulation of cardioblast differentiation; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cardiac muscle cell proliferation; sarcomere organization; spleen development; thyroid gland development; transcription from RNA polymerase II promoter; vasculogenesis; ventricular cardiac muscle cell development; ventricular cardiac myofibril development; Wnt receptor signaling pathway through beta-catenin

Disease: Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Hypoplastic Left Heart Syndrome 2; Hypothyroidism, Congenital, Nongoitrous, 5; Tetralogy Of Fallot; Ventricular Septal Defect 3
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 Research Articles on NKX2-5    1. Our findings demonstrate that somatic NKX2-5 mutations do not represent an important aetiologic pathway in Chinese children with congenital heart disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NKX2-5 antibodyPathways associated with anti-NKX2-5 antibody
 Reference Product  PubMed Publications
 GATA4 antibody  >377 publications with NKX2-5 and GATA4
 TBX5 antibody  >144 publications with NKX2-5 and TBX5
 MEF2C antibody  >127 publications with NKX2-5 and MEF2C
 BMP4 antibody  >40 publications with NKX2-5 and BMP4
 SRF antibody  >37 publications with NKX2-5 and SRF
 HAND2 antibody  >36 publications with NKX2-5 and HAND2
 HAND1 antibody  >27 publications with NKX2-5 and HAND1
 SMAD4 antibody  >10 publications with NKX2-5 and SMAD4
 MEF2A antibody  >8 publications with NKX2-5 and MEF2A
 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Conduction Pathway antibodies  Cardiac Conduction Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Physiological Factors Pathway antibodies  Physiological Factors Pathway Diagram
 Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies  Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
 SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway antibodies  SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway Diagram
 YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway antibodies  YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway Diagram
Diseases associated with anti-NKX2-5 antibodyOrgans/Tissues associated with anti-NKX2-5 antibody
 Disease Name  Pubmed Publications
 ATRIAL SEPTAL DEFECT 7 WITH OR WITHOUT ATRIOVENTRICULAR CONDUCTION DEFECTS Antibodies  >1710 publications with NKX2-5 and ATRIAL SEPTAL DEFECT 7 WITH OR WITHOUT ATRIOVENTRICULAR CONDUCTION DEFECTS
 Heart Diseases Antibodies  >418 publications with NKX2-5 and Heart Diseases
 Heart Defects, Congenital Antibodies  >264 publications with NKX2-5 and Heart Defects, Congenital
 Hypertrophy Antibodies  >53 publications with NKX2-5 and Hypertrophy
 Disease Models, Animal Antibodies  >46 publications with NKX2-5 and Disease Models, Animal
 Cardiomegaly Antibodies  >37 publications with NKX2-5 and Cardiomegaly
 Tetralogy of Fallot Antibodies  >37 publications with NKX2-5 and Tetralogy of Fallot
 Carcinoma Antibodies  >24 publications with NKX2-5 and Carcinoma
 Atrial Fibrillation Antibodies  >22 publications with NKX2-5 and Atrial Fibrillation
 Fibrosis Antibodies  >20 publications with NKX2-5 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1097 publications with NKX2-5 and Heart
 Embryonic Tissue Antibodies  >432 publications with NKX2-5 and Embryonic Tissue
 Brain Antibodies  >25 publications with NKX2-5 and Brain
 Lung Antibodies  >24 publications with NKX2-5 and Lung
 Liver Antibodies  >20 publications with NKX2-5 and Liver
 Spleen Antibodies  >16 publications with NKX2-5 and Spleen
 Intestine Antibodies  >9 publications with NKX2-5 and Intestine
 Uncharacterized Tissue Antibodies  >5 publications with NKX2-5 and Uncharacterized Tissue
 Ovary Antibodies  >1 publications with NKX2-5 and Ovary
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